Flavin reductase antibody

Principal name

Flavin reductase antibody

Alternative names for Flavin reductase antibody

Biliverdin reductase B, BLVRB, FLR, NADPH-dependent diaphorase, NADPH-flavin reductase, Biliverdin-IX beta-reductase, Green heme-binding protein

SwissProt ID

P30043 (Human), P52556 (Bovin), Q923D2 (Mouse)

Gene ID

645 (BLVRB), 233016 (Blvrb)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of Flavin reductase antibody

Methemoglobinemia is a blood disease originated from oxidization of hemoglobin-iron ions (Fe(II) → Fe(III)). Oxidized hemoglobin (methemoglobin) which can not carry oxygen, is rereduced by enzyme named methemoglobin reductase or administrated flavin or methylene blue. NADPH-flavin reductase (FLR) is a 21 kDa protein that catalyzes electron transfer fr om mainly NADPH to flavins (FMN, FAD) and a variety of other electron acceptors including methylene blue, 2,6-dichlorophenolindophenol (DCIP), and pyrroloquinoline quinone (PQQ). FLR was originally identified as a candidate of NADPH dependent methemoglobin reductase. However it was found that, though this enzyme reduces methemoglobin through reducing flavin or methylene blue, it does not reduce methemoglobin directly under physiological conditions. It was also reported that human FLR is identical with human biliverdin-IX β -reductase (BLVR-B) which participate in hem catabolism. Hem, which is a component of hemoglobin is metabolized to a biliverdin by hemoxygenase. Biliverdin-IX β , which is one of isomers of biliverdin is then reduced to bilirubin-IX β by BLVR-B. Though physiological functions for FLR have not been clear, one of the important functions may be the reduction of biliverdin-IX β.

General readings

L. Lee Grismer., et al. (2007). Herpetologica. 63(3):392-400.
Baranano DE., et al. (2002). Proc Natl Acad Sci USA. 10; 99(25):16093-8.

15 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Flavin reductase (C-term) antibody

  BLVRB Goat Polyclonal Antibody  
Anti-BLVRB antibody (0.3µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Flavin reductase (38-49) antibody

  BLVRB Goat Polyclonal Antibody  
Anti-BLVRB antibody (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Flavin reductase antibody

  Rabbit polyclonal Anti-BLVRB Antibody  
WB Suggested Anti-BLVRB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Flavin reductase antibody

Western blot analysis of NADPH-Flavin Reductase expression in Jurkat (1), Raji (2), HeLa (3), MRC-5 (4), ZR-75-1 (5), U937 (6), NIH/3T3 (7), WR19L (8), P19 (9) and Ba/F3 (10) using AM26613AF-N. Mouse IgG1 2C10 Azide Free Hu, Mky, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Blvrb - mouse gene knockout kit via CRISPR

Blvrb - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Blvrb - mouse gene knockout kit via CRISPR

Blvrb - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Blvrb - mouse gene knockout kit via CRISPR

Blvrb - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Blvrb - mouse gene knockout kit via CRISPR

Blvrb - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Flavin reductase

  Recombinant protein of human biliverdin reductase B (flavin reductase (NADPH)) (BLVRB)  
Flavin reductase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Flavin reductase (1-206)

Flavin reductase Human Purified > 95 % by SDS PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

Flavin reductase (1-206)

Flavin reductase Human Purified > 95 % by SDS PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, BLVRB (mGFP-tagged) - Human biliverdin reductase B (flavin reductase (NADPH)) (BLVRB), 200 uL, >10^7 TU/mL

Lenti ORF particles, BLVRB (mGFP-tagged) - Human biliverdin reductase B (flavin reductase (NADPH)) (BLVRB), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, BLVRB (Myc-DDK tagged) - Human biliverdin reductase B (flavin reductase (NADPH)) (BLVRB) , 200 uL, >10^7 TU/mL

Lenti ORF particles, BLVRB (Myc-DDK tagged) - Human biliverdin reductase B (flavin reductase (NADPH)) (BLVRB) , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Blvrb (GFP-tagged) - Mouse biliverdin reductase B (flavin reductase (NADPH)) (Blvrb), 200 uL, >10^7 TU/mL

Lenti ORF particles, Blvrb (GFP-tagged) - Mouse biliverdin reductase B (flavin reductase (NADPH)) (Blvrb), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Blvrb (Myc-DDK-tagged) - Mouse biliverdin reductase B (flavin reductase (NADPH)) (Blvrb), 200 uL, >10^7 TU/mL

Lenti ORF particles, Blvrb (Myc-DDK-tagged) - Mouse biliverdin reductase B (flavin reductase (NADPH)) (Blvrb), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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