FLOT1 (Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al. (1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al. (1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al. (2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.
Bickel PE et al. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J Biol Chem 272:13793-802 (1997). PubMed PMID: 9153235
GTX13493 staining (0.3µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
FLOT1 polyclonal antibody ( Cat # PAB6176 ) staining ( 0.3 µg/ml ) of NCI-H460 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Flotillin 1 antibody [C3], C-term immunoprecipitates Flotillin 1 protein in IP experiments.IP samples: HCT-116 whole cell extractA. Control with 4 μg of preimmune Rabbit IgGB. Immunoprecipitation of Flotillin 1 protein by 4 μg Flotillin 1 antibody [C3], C-term (GTX104769)10 % SDS-PAGEThe immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
1X10^6 HeLa cells were stained with 0.05ug Flotillin 1 antibody (15571-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IgG with dilution 1:100.
Western Blot: Flotillin-1 Antibody [NBP1-79022]-Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope.
Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-Flotillin 1 Polyclonal Antibody, Unconjugated (bs-7798R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.