Flotillin-1 / FLOT1 antibody

Principal name

Flotillin-1 / FLOT1 antibody

Alternative names for Flotillin-1 / FLOT1 antibody

Reggie-2, Reg2, REG-2

SwissProt ID

O08917 (Mouse), O75955 (Human), Q08DN8 (Bovin), Q767L6 (Pig), Q9Z1E1 (Rat)

Gene ID

10211 (FLOT1), 1021131 (O52377)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Por (Porcine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Gt (Goat), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Flotillin-1 / FLOT1 antibody

FLOT1 (Flotillin 1), is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the FLOT1 gene to chromosome 6. Bickel et al. (1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al. (1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al. (2005) found that prostate tumor overexpressed gene-1 interacted with flotillin-1 in detergent-insoluble membrane fractions. Flotillin-1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.

General readings

Bickel PE et al. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J Biol Chem 272:13793-802 (1997). PubMed PMID: 9153235

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Flotillin-1 / FLOT1 (C-term) antibody

TA302489 staining (0.3µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Flotillin-1 / FLOT1 antibody

WB Suggested Anti-FLOT1 Antibody; Titration: 1.0 ug/ml; Positive Control: Hela Whole cell Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Flotillin-1 / FLOT1 antibody

WB Suggested Anti-FLOT1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Flotillin-1 / FLOT1 antibody

FLOT1 MaxPab rabbit polyclonal antibody. Western Blot analysis of FLOT1 expression in human liver. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Flotillin-1 / FLOT1 antibody

Flotillin-1 / FLOT1 Mouse IgM 4A1 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Flotillin-1 / FLOT1 antibody

HEK293 overexpressing FLOT1 and probed with FLOT1 polyclonal antibody ( Cat # PAB6176 ) ( mock transfection in first lane ), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Flotillin-1 / FLOT1 antibody

Flotillin-1 / FLOT1 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

Flotillin-1 / FLOT1 antibody

NB 100-1043 staining (0.3µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Flotillin-1 / FLOT1 antibody

Western Blot: Flotillin-1 Antibody [NBP1-79022]-Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-Flotillin-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  Flotillin-1 was also immunoprecipitated by rabbit anti-Flotillin-1 antibody NBP1-79021 which recognizes an upstream epitope. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Flotillin-1 / FLOT1 antibody

Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498] - Immunohistochemical staining of human stomach shows distinct cytoplasmic and membranous positivity with a granular pattern. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Flotillin-1 / FLOT1

Flotillin-1 / FLOT1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Flotillin-1 / FLOT1

Flotillin-1 / FLOT1 Human
2 µg / €300.00
  Abnova Taiwan Corp.

Flotillin-1 / FLOT1

Flotillin-1 / FLOT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Flotillin-1 / FLOT1

Flotillin-1 / FLOT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

14 Item(s)

per page
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