Flotillin-2 / FLOT2 antibody

Principal name

Flotillin-2 / FLOT2 antibody

Alternative names for Flotillin-2 / FLOT2 antibody

Epidermal surface antigen, ESA, ESA1, M17S1, Reggie-1, Reg1, REG-1

SwissProt ID

Q14254 (Human), Q60634 (Mouse), Q9BTI6 (Human), Q9Z2S9 (Rat)

Gene ID

2319 (FLOT2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Goat, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Flotillin-2 / FLOT2 antibody

Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry a evolutionarily conserved domain called the prohibitin homology domain (PHB). Flotillin members are ubiquitously expressed and located to non-caveolar microdomains (lipid rafts) on the cell plasma membrane where they support signal transduction and regulate lipid raft motility and localization. Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found at compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, as well as the nucleus. Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome. Both flotillin-1 and flotillin-2 are commonly used as lipid raft associated markers.

General readings

Rajendran L, Masilamani M, Solomon S, Tikkanen R, Stuermer CA, Plattner H, Illges H.
Asymmetric localization of flotillins/reggies in preassembled platforms confers inherent polarity to hematopoietic cells.
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8241-6. Epub 2003 Jun 25.
PMID: 12826615

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Flotillin-2 / FLOT2 (C-term) antibody

Flotillin 2 antibody [C3], C-term detects FLOT2 protein by Western blot analysis. A. 50 ug mouse brain lysate/extract. 10 % SDS-PAGE. Flotillin 2 antibody [C3], C-term (TA308097) dilution: 1:1000 Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Flotillin-2 / FLOT2 (C-term) antibody

TA302490 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Flotillin-2 / FLOT2 (415-428) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bov, Bt, Can, Hst, Hu, Mky, Ms, Por, Prim, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Flotillin-2 / FLOT2 antibody

Immunoperoxidase of purified MaxPab antibody to FLOT2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml] Mouse Serum Hu E(detection), P, WB
50 µl / €370.00
  Abnova Taiwan Corp.

Flotillin-2 / FLOT2 antibody

Immunoperoxidase of purified MaxPab antibody to FLOT2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml] Mouse Purified Hu E(detection), P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Flotillin-2 / FLOT2 antibody

Western Blot analysis of FLOT2 expression in transfected 293T cell line (H00002319-T02) by FLOT2 MaxPab polyclonal antibody.

Lane 1: FLOT2 transfected lysate(41.69 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Flotillin-2 / FLOT2 antibody

Flotillin-2 / FLOT2 Mouse IgG2a 3G6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Flotillin-2 / FLOT2 antibody

FLOT2 polyclonal antibody ( Cat # PAB6175 ) staining ( 0.1 µg/ml ) of human brain ( cerebellum) lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Flotillin-2 / FLOT2 antibody

Flotillin-2 / FLOT2 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

Flotillin-2 / FLOT2 antibody

Western blot analysis of Flotillin 2 expression in A431 (A); mouse brain (B); rat brain (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Flotillin-2 / FLOT2 antibody

Western blot analysis of Flotillin 2 expression in A431 (A); mouse brain (B); rat brain (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Flotillin-2 / FLOT2 antibody

Western blot analysis of Flotillin 2 expression in A431 (A); mouse brain (B); rat brain (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Flotillin-2 / FLOT2 antibody

NB 300-922 (0.3ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Flotillin-2 / FLOT2 antibody

Flotillin-2 / FLOT2 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Flotillin-2 / FLOT2 antibody

Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-84910] - Immunohistochemical staining of human prostate shows strong membranous and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Flotillin-2 / FLOT2

Flotillin-2 / FLOT2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Flotillin-2 / FLOT2

Flotillin-2 / FLOT2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Flotillin-2 / FLOT2

Flotillin-2 / FLOT2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Flotillin-2 / FLOT2

Flotillin-2 / FLOT2 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

Flotillin-2 / FLOT2

Flotillin-2 / FLOT2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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