Flt3 ligand antibody

Principal name

Flt3 ligand antibody

Alternative names for Flt3 ligand antibody

SL cytokine, FLT3LG, Flt3L, fms-related tyrosine kinase 3 ligand

SwissProt ID

P49771 (Human), P49772 (Mouse)

Gene ID

2323 (FLT3LG), 14256 (Flt3l)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Eq (Equine), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C), Neutralisation (NEUT)

Background of Flt3 ligand antibody

Fms-like tyrosine kinase 3 (Flt-3) and its cognate ligand, Flt-3 ligand (Flt-3L), are essential growth factors involved in the survival and differentiation of hematopoietic progenitor and stem cells, specifically the development of NK cells, pre-B and pre-T cells, monocytes/macrophages and dendritic cells. Activation of Flt-3 promotes the survival and proliferation of early progenitor cells. Flt-3 receptor responses are cell type dependent and also influenced by other growth factors such as GM-CSF, G-CSF, IL-3 and Epo. Furthermore, Flt-3 is involved in lymphocyte development in conjunction with cytokines such as IL-3, IL-7 and IL-11. Expression of Flt-3 has been reported in peripheral blood mononuclear cells, thymus and non-immune somatic cells in the heart, lung, spleen, prostate, kidney, pancreas, skeletal muscle, intestine and liver. Both Flt-3 and Flt-3L play vital roles in the development of a number of human leukocyte malignancies including acute myelogenous leukemia (AML), acute promyelocytic leukemia (APL), B and T cell acute lymphoblastic leukemia (ALL), chronic myelogenous leukemia (CML) and myelodysplasia (MDS). Flt-3L also has a capacity to expand dendritic cells and may facilitate vaccine-induced immune responses against cancer and infectious pathogens. Additionally, due to its role in the development of NK cells, Flt-3L is also effective in promoting immunity against intracellular viral and non-viral pathogens. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Drexler HG & Quentmeier H (2004) Growth Factors 22: 71-3.

Items 1 to 20 of 62 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Flt3 ligand antibody

Western blot analysis of extracts from Jurkat cells, using Flt3 ligand antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Flt3 ligand antibody

Anti-FLT3LG mouse monoclonal antibody (TA500274) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FLT3LG(RC222242). Mouse IgG1 OTI8D10 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Flt3 ligand antibody

Western blot analysis of Flt3-L antibody (Cat.-No. AP20420PU-N) at 1/500 dilution: Lane 1: Hela whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: PC12 whole cell lysate. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Flt3 ligand antibody

Flt3 ligand Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

Flt3 ligand antibody

Flt3 ligand Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

Flt3 ligand antibody

Flt3 ligand Rabbit Aff - Purified Hu E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

Flt3 ligand antibody

Flt3 ligand Rabbit Aff - Purified Hu E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Flt3 ligand antibody

Flt3 ligand Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Flt3 ligand antibody

Immunofluorescence of purified MaxPab antibody to FLT3LG on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

Flt3 ligand antibody

Immunofluorescence of purified MaxPab antibody to FLT3LG on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Flt3 ligand antibody

Western Blot analysis of FLT3LG expression in transfected 293T cell line (H00002323-T01) by FLT3LG MaxPab polyclonal antibody.

Lane 1: FLT3LG transfected lysate(26.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Flt3 ligand antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between KITLG and FLT3LG. HeLa cells were stained with anti-KITLG rabbit purified polyclonal 1:1200 and anti-FLT3LG mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 4C4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Flt3 ligand antibody

Western blot analysis of extracts from Jurkat cells, using Flt3 ligand Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

Flt3 ligand antibody

Western blot analysis of extracts from Jurkat cells, using Flt3 ligand Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

Flt3 ligand antibody

Human Muscle; WB Suggested Anti-FLT3LG Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; FLT3LG antibody - N-terminal region (ARP45110_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Flt3 ligand antibody

Flt3 ligand Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

Flt3 ligand antibody

Immunohistochemistry: FIt3-L Antibody (EP1140Y) [NB110-56993] - Immunohistochemical staining of paraffin-embedded human tonsils using anti-Flt3-L (NB110-56993. Rabbit IgG EP1140Y Aff - Purified Hu F, ICC/IF, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

Flt3 ligand antibody

Western Blot: Flt3-L Antibody [NBP1-19575] - extracts from Jurkat cells. Rabbit Aff - Purified Hu C, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Flt3 ligand antibody

Immunofluorescence: Flt3 ligand Antibody (8D10) [NBP1-47756] - Anti-FLT3LG mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FLT3LG. Mouse 8D10 Aff - Purified Hu ICC/IF, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Flt3 ligand

Flt3 ligand Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Items 1 to 20 of 62 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  • LinkedIn