Folate receptor alpha antibody
Principal name
Folate receptor alpha antibody
Alternative names for Folate receptor alpha antibody
FR-alpha, Folate receptor 1, MOv18, KB cells FBP, FOLR1, FOLR, Adult folate-binding protein, Ovarian tumor-associated antigen MOv18
Gene ID
2348 (FOLR1)
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)
Background of Folate receptor alpha antibody
Folate is an essential vitamin that must be obtained from food intake through intestinal absorption in mammals. Folate and reduced folic acid derivatives bind to the folate receptor (FR) family, which mediates the endocytosis of 5-methyltetrahydrofolate into the cell. The folate receptors consist of five members, α, β, γ, γ’ (which is produced by alternative splicing) and δ. β-FR is attached to the membrane by a GPI anchor and is expressed in malignant tissues of epithelial and nonepithelial origin. γ-FR is expressed in tissues of hematopoietic origin, such as spleen, thymus and bone marrow. The expression pattern of δ-FR is elusive, which suggests that it is highly restricted both spatially and temporally. The α isoform of the folate receptor is used as a highly selective tumor marker and may be targeted for the delivery of therapeutic compounds to tumor cells by coupling to derivatives of folic acid.
General readings
Sivakumaran, S., et al. J. Steroid Biochem. Mol. Biol. 122(5):333-340(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
O'Byrne, M.R., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(8):689-694(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010)
Elwood, P.C., et al. Biochemistry 36(6):1467-1478(1997)
Western Blot analysis of FOLR1 expression in transfected 293T cell line (H00002348-T01) by FOLR1 MaxPab polyclonal antibody.Lane 1: FOLR1 transfected lysate(28.38 KDa).Lane 2: Non-transfected lysate.
Human Lung Tissue; Rabbit Anti-FOLR1 Antibody . Catalog Number: ARP41427_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue . Observed Staining: Membrane and cytoplasmic in alveolar type I & II cells . Primary Antibody Concentration: 1:100 . Other Working Concentrations: 1/600 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-FOLR1 Antibody (ARP41427_P050) ) in Human Lung Tissue using Immunohistochemistry
PANC1 Whole Cell; Host: Rabbit. Target Name:FOLR1. Sample Tissue:PANC1 Whole Cell. Lane A: Primary Antibody . Lane B:Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/Lane. Gel Concentration: 0.12.; FOLR1 antibody - middle region (ARP41427_P050) in PANC1 Whole Cell cells using Western Blot
PANC1; WB Suggested Anti-FOLR1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: PANC1 cell lysate. FOLR1 is supported by BioGPS gene expression data to be expressed in PANC1.; FOLR1 antibody - middle region (ARP41427_P050) in PANC1 cells using Western Blot
Western blot (WB) analysis of FR-α antibody (Cat.-No.: AP21174PU-N) in extracts from HUVEC cells.
Western Blot of goat anti-Folate binding protein /Folate receptor alpha antibody biotin conjugated . Lane 1: Blotto. Load: 50.0 µg per lane. Primary antibody: Goat anti-Folate receptor alpha antibody biotin conjugated Cat.-No. R1080B at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block for 30 minutes at RT. Predicted/observed size: 28 kDa, 30 kDa for Folate receptor alpha. Migrates slightly higher. Other band(s): non-specific proteins in Blotto.
Western blot analysis of FR alpha expression in A431 (A); HeLa (B); HepG2 (C); JAR (D) whole cell lysates.
Representative Standard Curve
Representative Standard Curve
Western blot analysis of extracts from HUVEC cells, LOVO cells and K562 cells, using FOLR1 antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25uM, 60mins), using Aggrecan (Cleaved-Asp369) antibody (#TA313889).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Aggrecan (Cleaved-Asp369) antibody (#TA313889).The picture on the right is treated with the synthesized peptide.
Western blot analysis of lysate from Hela cell line, using FOLR1 Antibody (N-term)(Cat. #TA324367). TA324367 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
FOLR1 antibody (N-term) (Cat. #TA324367) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FOLR1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
FOLR1 Antibody (N-term) (Cat. #TA324367) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-FOLR1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: PANC1 cell lysateFOLR1 is supported by BioGPS gene expression data to be expressed in PANC1
Host: Rabbit; Target Name: FOLR1; Sample Tissue: PANC1 Whole Cell; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide; Primary Antibody Concentration: 1 ug/ml; Peptide Concentration: 5 ug/ml; Lysate Quantity: 25 ug/lane/Lane; Gel Concentration: 0.12
Rabbit Anti-FOLR1 Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue; Observed Staining: Membrane and cytoplasmic in alveolar type I & II cells; Primary Antibody Concentration: 1: 100; Other Working Concentrations: 1/600; Secondary Antibody: Donkey anti-Rabbit-Cy3; Secondary Antibody Concentration: 1: 200; Magnification: 20X; Exposure Time: 0.5 - 2.0 sec;
Staining of Folate Binding Protein (FBP) Clone LK26, on frozen human placenta tissue.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313888 | Folate receptor alpha antibody |
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| Rabbit polyclonal anti-FOLR1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA313889 | Folate receptor alpha antibody |
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| Rabbit polyclonal Aggrecan (Cleaved-Asp369) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA324367 | Folate receptor alpha (N-term) antibody |
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| Rabbit polyclonal FOLR1 Antibody (N-term) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, P, WB |
0.4 ml /
€410.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA346052 | Folate receptor alpha antibody |
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| Rabbit Polyclonal Anti-FOLR1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rb, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AM01125PU-N | Folate receptor alpha antibody |
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 |
Mouse | IgG1 | SF55 (BGN/1210/55) | Purified | Bov, Hu | E |
0.2 mg /
€360.00
|
| OriGene Technologies GmbH | |||||||
| AP21174PU-N | Folate receptor alpha antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | WB |
0.1 mg /
€350.00
|
||
| OriGene Technologies GmbH | |||||||
| BM965 | Folate receptor alpha antibody |
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Mouse | IgG2b | 1210/75 | Purified | Hu | E |
0.2 mg /
€350.00
|
| OriGene Technologies GmbH | |||||||
| BM966 | Folate receptor alpha antibody |
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|
Mouse | IgG1 | SF64 (BGN/1210/64) | Purified | Hu | E |
0.2 mg /
€370.00
|
| OriGene Technologies GmbH | |||||||
| R1080 | Folate receptor alpha antibody |
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Goat | Serum | Bov | E, WB |
2 ml /
€1,180.00
|
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| OriGene Technologies GmbH | |||||||
| R1080BS | Folate receptor alpha antibody |
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 |
Goat | Biotin | Bov | E, IP, WB |
0.1 mg /
€460.00
|
||
| OriGene Technologies GmbH | |||||||
| R1080HRPS | Folate receptor alpha antibody |
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|
Goat | HRP | Bov | E, IP, WB |
0.1 mg /
€460.00
|
||
| OriGene Technologies GmbH | |||||||
| R1080PS | Folate receptor alpha antibody |
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|
Goat | Purified | Bov | E, IP, WB |
0.1 mg /
€460.00
|
||
| OriGene Technologies GmbH | |||||||
| C15761-1 | Folate receptor alpha antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | E, WB |
50 µg /
€230.00
|
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| Assay Biotech | |||||||
| C15761-2 | Folate receptor alpha antibody |
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Rabbit | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€330.00
|
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| Assay Biotech | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN212291 | FOLR1 - human gene knockout kit via CRISPR |
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|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN212291BN | FOLR1 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN212291LP | FOLR1 - human gene knockout kit via CRISPR |
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|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN212291RB | FOLR1 - human gene knockout kit via CRISPR |
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|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| AR00063PU-N | Folate receptor alpha |
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|
Bovine | Purified | >95% pure (HPLC) | Milk |
1 mg /
€770.00
|
| OriGene Technologies GmbH | |||||
| AR51758PU-N | Folate receptor alpha (25-234, His-tag) |
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 |
Human | Purified | > 85 % by SDS - PAGE | E. coli |
0.5 mg /
€730.00
|
| OriGene Technologies GmbH | |||||
