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Folate receptor alpha antibody

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Principal name

Folate receptor alpha antibody

Alternative names for Folate receptor alpha antibody

FR-alpha, Folate receptor 1, MOv18, KB cells FBP, FOLR1, FOLR, Adult folate-binding protein, Ovarian tumor-associated antigen MOv18

SwissProt ID

P02702 (Bovin), P15328 (Human), P35846 (Mouse)

Gene ID

2348 (FOLR1)

Ncbi ID

9913, NP_000793.1, 9606, NP_000793, NP_057936, 10090, NM_000802, NM_016724, NM_016729

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of Folate receptor alpha antibody

Folate is an essential vitamin that must be obtained from food intake through intestinal absorption in mammals. Folate and reduced folic acid derivatives bind to the folate receptor (FR) family, which mediates the endocytosis of 5-methyltetrahydrofolate into the cell. The folate receptors consist of five members, α, β, γ, γ’ (which is produced by alternative splicing) and δ. β-FR is attached to the membrane by a GPI anchor and is expressed in malignant tissues of epithelial and nonepithelial origin. γ-FR is expressed in tissues of hematopoietic origin, such as spleen, thymus and bone marrow. The expression pattern of δ-FR is elusive, which suggests that it is highly restricted both spatially and temporally. The α isoform of the folate receptor is used as a highly selective tumor marker and may be targeted for the delivery of therapeutic compounds to tumor cells by coupling to derivatives of folic acid.

General readings

Sivakumaran, S., et al. J. Steroid Biochem. Mol. Biol. 122(5):333-340(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
O'Byrne, M.R., et al. Birth Defects Res. Part A Clin. Mol. Teratol. 88(8):689-694(2010)
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010)
Elwood, P.C., et al. Biochemistry 36(6):1467-1478(1997)

H00002348-B01P - Folate receptor alpha
Western Blot analysis of FOLR1 expression in transfected 293T cell line (H00002348-T01) by FOLR1 MaxPab polyclonal antibody.Lane 1: FOLR1 transfected lysate(28.38 KDa).Lane 2: Non-transfected lysate.
ARP41427_P050 - Folate receptor alpha
Human Lung Tissue; Rabbit Anti-FOLR1 Antibody . Catalog Number: ARP41427_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue . Observed Staining: Membrane and cytoplasmic in alveolar type I & II cells . Primary Antibody Concentration: 1:100 . Other Working Concentrations: 1/600 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-FOLR1 Antibody (ARP41427_P050) ) in Human Lung Tissue using Immunohistochemistry
ARP41427_P050 - Folate receptor alpha
PANC1 Whole Cell; Host: Rabbit. Target Name:FOLR1. Sample Tissue:PANC1 Whole Cell. Lane A: Primary Antibody . Lane B:Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1ug/ml. Peptide Concentration: 5ug/ml. Lysate Quantity: 25ug/lane/Lane. Gel Concentration: 0.12.; FOLR1 antibody - middle region (ARP41427_P050) in PANC1 Whole Cell cells using Western Blot
ARP41427_P050 - Folate receptor alpha
PANC1; WB Suggested Anti-FOLR1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: PANC1 cell lysate. FOLR1 is supported by BioGPS gene expression data to be expressed in PANC1.; FOLR1 antibody - middle region (ARP41427_P050) in PANC1 cells using Western Blot
AP21174PU-N - Folate receptor alpha
Western blot (WB) analysis of FR-α antibody (Cat.-No.: AP21174PU-N) in extracts from HUVEC cells.
C15761-1 - Folate receptor alpha
Western blot analysis of extracts from K562/LOVO/HuvEc cells, using FOLR1 Antibody. The lane on the right is treated with the synthesized peptide.
R1080BS - Folate receptor alpha
Western Blot of goat anti-Folate binding protein /Folate receptor alpha antibody biotin conjugated . Lane 1: Blotto. Load: 50.0 µg per lane. Primary antibody: Goat anti-Folate receptor alpha antibody biotin conjugated Cat.-No. R1080B at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block for 30 minutes at RT. Predicted/observed size: 28 kDa, 30 kDa for Folate receptor alpha. Migrates slightly higher. Other band(s): non-specific proteins in Blotto.
AR51758PU-N - Folate receptor alpha
CPA1436-100ul - Folate receptor alpha
Western blot analysis of FR alpha expression in A431 (A); HeLa (B); HepG2 (C); JAR (D) whole cell lysates.
CEK1163 - Human FR alpha ELISA Kit
Representative Standard Curve
CEK1463 - Mouse FR alpha ELISA Kit
Representative Standard Curve
TA313888 - Folate receptor alpha
Western blot analysis of extracts from HUVEC cells, LOVO cells and K562 cells, using FOLR1 antibody.The lane on the right is treated with the synthesized peptide.
TA313889 - Folate receptor alpha
Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25uM, 60mins), using Aggrecan (Cleaved-Asp369) antibody (#TA313889).The lane on the right is treated with the synthesized peptide.
TA313889 - Folate receptor alpha
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Aggrecan (Cleaved-Asp369) antibody (#TA313889).The picture on the right is treated with the synthesized peptide.
TA324367 - Folate receptor alpha
Western blot analysis of lysate from Hela cell line, using FOLR1 Antibody (N-term)(Cat. #TA324367). TA324367 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
TA324367 - Folate receptor alpha
FOLR1 antibody (N-term) (Cat. #TA324367) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FOLR1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
TA324367 - Folate receptor alpha
FOLR1 Antibody (N-term) (Cat. #TA324367) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA346052 - Folate receptor alpha
WB Suggested Anti-FOLR1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: PANC1 cell lysateFOLR1 is supported by BioGPS gene expression data to be expressed in PANC1
TA346052 - Folate receptor alpha
Host: Rabbit; Target Name: FOLR1; Sample Tissue: PANC1 Whole Cell; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide; Primary Antibody Concentration: 1 ug/ml; Peptide Concentration: 5 ug/ml; Lysate Quantity: 25 ug/lane/Lane; Gel Concentration: 0.12
TA346052 - Folate receptor alpha
Rabbit Anti-FOLR1 Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue; Observed Staining: Membrane and cytoplasmic in alveolar type I & II cells; Primary Antibody Concentration: 1: 100; Other Working Concentrations: 1/600; Secondary Antibody: Donkey anti-Rabbit-Cy3; Secondary Antibody Concentration: 1: 200; Magnification: 20X; Exposure Time: 0.5 - 2.0 sec;
TA352024 - Folate receptor alpha
Staining of Folate Binding Protein (FBP) Clone LK26, on frozen human placenta tissue.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Folate receptor alpha antibody
  Rabbit polyclonal anti-FOLR1 antibody  
Western blot analysis of extracts from HUVEC cells, LOVO cells and K562 cells, using FOLR1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Folate receptor alpha antibody
  Rabbit polyclonal Aggrecan (Cleaved-Asp369) antibody  
Western blot analysis of extracts from Jurkat cells, treated with Etoposide (25uM, 60mins), using Aggrecan (Cleaved-Asp369) antibody (#TA313889).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

Folate receptor alpha (N-term) antibody
  Rabbit polyclonal FOLR1 Antibody (N-term)  
Western blot analysis of lysate from Hela cell line, using FOLR1 Antibody (N-term)(Cat. #TA324367). TA324367 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.
+2 additional images

Folate receptor alpha antibody
  Rabbit Polyclonal Anti-FOLR1 Antibody  
WB Suggested Anti-FOLR1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: PANC1 cell lysateFOLR1 is supported by BioGPS gene expression data to be expressed in PANC1 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.
+2 additional images

Folate receptor alpha antibody
  Mouse Monoclonal anti-Folate Binding Protein Antibody  
Staining of Folate Binding Protein (FBP) Clone LK26, on frozen human placenta tissue. Mouse IgG2a LK26 Purified Hu IP, P
1 ml / €579.00
  OriGene Technologies, Inc.

Folate receptor alpha antibody
Folate receptor alpha Mouse IgG1 SF55 (BGN/1210/55) Purified Bov, Hu E
0.2 mg / €350.00
  Acris Antibodies GmbH

Folate receptor alpha antibody
Western blot (WB) analysis of FR-α antibody (Cat.-No.: AP21174PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Folate receptor alpha antibody
Folate receptor alpha Mouse IgG2b 1210/75 Purified Hu E
0.2 mg / €340.00
  Acris Antibodies GmbH

Folate receptor alpha antibody
Folate receptor alpha Mouse IgG1 SF64 (BGN/1210/64) Purified Hu E
0.2 mg / €360.00
  Acris Antibodies GmbH

Folate receptor alpha antibody
Folate receptor alpha Goat Serum Bov E, WB
2 ml / €1,180.00
  Acris Antibodies GmbH

Folate receptor alpha antibody
Western Blot of goat anti-Folate binding protein /Folate receptor alpha antibody biotin conjugated . Lane 1: Blotto. Load: 50.0 µg per lane. Primary antibody: Goat anti-Folate receptor alpha antibody biotin conjugated Cat.-No. R1080B at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block for 30 minutes at RT. Predicted/observed size: 28 kDa, 30 kDa for Folate receptor alpha. Migrates slightly higher. Other band(s): non-specific proteins in Blotto. Goat Biotin Bov E, IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Folate receptor alpha antibody
Folate receptor alpha Goat HRP Bov E, IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Folate receptor alpha antibody
Folate receptor alpha Goat Purified Bov E, IP, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

Folate receptor alpha antibody
Western blot analysis of extracts from K562/LOVO/HuvEc cells, using FOLR1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

Folate receptor alpha antibody
Western blot analysis of extracts from K562/LOVO/HuvEc cells, using FOLR1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

FOLR1 - human gene knockout kit via CRISPR
FOLR1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

FOLR1 - human gene knockout kit via CRISPR
FOLR1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

FOLR1 - human gene knockout kit via CRISPR
FOLR1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

FOLR1 - human gene knockout kit via CRISPR
FOLR1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Folate receptor alpha
Folate receptor alpha Bovine Purified >95% pure (HPLC) Milk
1 mg / €730.00
  Acris Antibodies GmbH

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