FOXC2 is a member of forkhead/winged helix transcription factor family whose members serve as key regulators in embryogenesis and cell differentiation. FOXC2 functions as a key regulator of adipocyte metabolism by increasing the sensitivity of the beta-adrenergic-cAMP-protein kinase A (PKA) signaling pathway through alteration of adipocyte PKA holoenzyme composition. Increased FOXC2 levels, induced by high fat diet, seem to counteract most of the symptoms associated with obesity. FOXC2 expression is also associated with the early stage of chondrogenic differentiation both in vivo and in vitro. FOXC2 haploinsufficiency results in Lymphedema-distichiasis (LD), an autosomal dominant disorder that classically presents as lymphedema of the limbs and double rows of eyelashes (distichiasis). Mutant mice null for FOXC2 show defects in axial and cranial skeletogenesis, suggesting a requirement of FOXC2 for skeletal tissue development.
Dagenais, S.L. et al Foxc2 is expressed in developing lymphatic vessels and other tissues associated with lymphedema-distichiasis syndrome Gene Expression Patterns :1-9 (2004).General / background references:Zhou Y et al. Identification of FOXC1 as a TGF-beta1 responsive gene and its involvement in negative regulation of cell growth. Genomics 80:465-72 (2002). PubMed PMID: 12408963
Human HepG2; WB Suggested Anti-FOXC1 Antibody Titration: 5.0ug/ml. Positive Control: HepG2 cell lysate; FOXC1 antibody - C-terminal region (ARP32300_T100) in Human HepG2 cells using Western Blot
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with GTX25079 (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with GTX25079 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
FOXC1 polyclonal antibody ( Cat # PAB6425 ) staining ( 0.5 µg/ml ) of human bone marrow lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with FOXC1 Antibody Cat.-No AP16141PU-N (1 µg/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with AP16141PU-N (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. AP16141PU-N (4 µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Human THP-1; WB Suggested Anti-FOXC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; FOXC1 antibody - N-terminal region (ARP38036_P050) in Human THP-1 cells using Western Blot
Human Jurkat; WB Suggested Anti-FOXC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; FOXC1 antibody - C-terminal region (ARP38037_P050) in Human Jurkat cells using Western Blot
Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with FOXC1 antibody AP22489PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Spleen (formalin-fixed, paraffin-embedded) stained with FOXC1 antibody AP22489PU-N at 3.75 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry-Paraffin: FOXC1 Antibody [NBP1-50197] - FOXC1 antibody (1:200) was used in IHC to stain formalin-fixed, paraffin-embedded human colon, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunohistochemistry-Paraffin: FOXC1 Antibody [NBP1-89025] - Immunohistochemical staining of human testis shows moderate nuclear positivity in spermatogonia of seminferous ducts and moderate cytoplasmic in spermatids.
FOXC1 Antibody (C-term) (GTX80948) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of FOXC1/2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of FOXC1/2 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).