FOXO3 / FKHRL1 antibody

Principal name

FOXO3 / FKHRL1 antibody

Alternative names for FOXO3 / FKHRL1 antibody

FOXO3A, AF6q21 protein, Forkhead box protein O3, Forkhead in rhabdomyosarcoma-like 1

SwissProt ID

O43524 (Human), Q6EUW1 (Xenla)

Gene ID

2309 (FOXO3), 8623096 (abcG5)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Por (Porcine), Rt (Rat), African clawed frog, Bov (Bovine), Chk (Chicken), Mky (Monkey), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Frozen Sections (C)

Background of FOXO3 / FKHRL1 antibody

The superfamily of Forkhead transcription factors (FOX) consists of more than 100 members, with orthologues expressed in a variety of species ranging from yeast to man. They are characterized by a common Forkhead (or Winged Helix) domain, a variant of the helix-turn-helix motif. Forkhead family members have been shown to play key regulatory roles in embryonic development, differentiation, apoptosis, and tumorigenesis. Three Forkhead family members, termed FKHR (FOXO1a), FKHRL1 (FOXO3a), and AFX (FOXO4) were first identified at chromosomal breakpoints in human tumors, and consequently linked to tumorigenesis. It is now well established that these proteins are targets of the PI3K/PKB pathway. PI3K/PKB (Phosphatydil Inositol 3-kinase/Protein Kinase B or Akt) plays a role in oncogenic transformation. PKB/Akt substrates include components of the cell death machinery, such as BAD and caspase 9.1,9,10 Stimulation of this cascade by Nerve Growth Factor or IGF-1 leads to phosphorylation of these proteins and suppression of their proapoptotic function, partially explaining the survival effect of PKB. The identification of the transcription factor DAF16 as a PKB target in the nematode C.elegans was critical in the understanding of its link with FKHR, FKHRL1, and AFX.13 DAF16 belongs to the Forkhead family and transduces insulin-like signals. FKHR, FKHRL1, and AFX (FOXO4)1,5 are similar in sequence to DAF16 and represent the mammalian counterparts. Similarly, these proteins are PKB/AKT targets. Growth factors regulate the activity of FKHRL1, FKHR, and AFX via the PKB/PI3K pathway, by direct phosphorylation of the transcription factors. These transcription factors are inhibited by phosphorylation by PKB, the most likely mechanism being regulation of nuclear localization. In FKHRL1 (FOX3a), there are three PKB phosphorylation consensus sites, Thr32, Ser253, and Ser315. Thr32 and Ser253 are phosphorylated by PKB after induction by survival factors such as IGF-1. This results in FKHRL1 retention in the cytoplasm, and/or nuclear exclusion, and consequent inhibition of FKHRL1-dependent transcription. Survival factor withdrawal induces FKHRL1 dephosphorylation and translocation to the nucleus. Within the nucleus, the dephosphorylated FKHRL1 induces target genes such as Fas ligand, and triggers apoptosis. Growth factors suppress the transcription of death genes by triggering PKB/AKT dependent phosphorylation and inactivation of FKHRL1, and thereby promote cell survival. A similar mechanism is proposed for FKHR and AFX. It has been shown that FKHRL1/FOXO3a modulates the expression of several genes that regulate DNA repair in response to stress at the G2-M checkpoint, oxidative stress resistance, and aging. Other genes that are regulated by FKHRL1 include mitotic genes such as cyclin B and polo-like kinase (plk). Antibodies reacting specifically with FKHRL1 (FOXO3) may be useful in studying the expression and function of the protein, as well as for correlating their expression pattern with physiological functions or pathological conditions.

General readings

Kops GJ & Burgering BM Forkhead transcription factors: new insights into protein kinase B (c-akt) signaling. J Mol Med 77:656-65 (1999). PubMed PMID: 10569203
Mansouri A The role of Pax3 and Pax7 in development and cancer. Crit Rev Oncog 9:141-9 (1998). PubMed PMID: 9973247
Anderson MJ et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics 47:187-99 (1998). PubMed PMID: 9479491
Brennan RG The winged-helix DNA-binding motif: another helix-turn-helix takeoff. Cell 74:773-6 (1993). PubMed PMID: 8374950

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FOXO3 / FKHRL1 control peptide

FOXO3 / FKHRL1
50 µg / €160.00
  Acris Antibodies GmbH

FOXO3 / FKHRL1 antibody

Hum. Adult Placenta; Host: Rabbit . Target Name: FOXO3 . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; FOXO3 antibody - N-terminal region (ARP38040_P050) in Hum. Adult Placenta cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

FOXO3 / FKHRL1 (N-term) antibody

AP15560PU FOXO3A antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

FOXO3 / FKHRL1 (N-term) antibody

AP15560PU FOXO3A antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

FOXO3 / FKHRL1 (N-term) antibody

AP15560PU FOXO3A antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

FOXO3 / FKHRL1 (C-term) antibody

TA302661 staining (0.5µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FOXO3 / FKHRL1 (N-term) antibody

Western blot analysis of FOXO3 in A-20 cell lysate with FOXO3 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FOXO3 / FKHRL1 antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human cervical cancer tissue. Recommended dilution: 1/50-150  The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using FOXO3 antibody at dilution 1/22, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

FOXO3 / FKHRL1 antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human stomach and lung cancer tissue. Recommended dilution: 1/50-150  The image on the left is immunohistochemistry of paraffin-embedded Human stomach and lung cancer tissue using FOXO3 antibody at dilution 1/17, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

FOXO3 / FKHRL1 antibody

FOXO3  Antibody (N-term) (Cat. #TA324516) western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).This demonstrates the FOXO3  antibody detected the FOXO3  protein (arrow). Rabbit IgG Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

FOXO3 / FKHRL1 antibody

Western blot analysis of FKHRL1 expression in serum treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FOXO3 / FKHRL1 antibody

Jurkat cell  extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit polyclonal antibody against FOXO3A. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6298 Purified Hu WB
50 µl / €325.00
  OriGene Technologies, Inc.

FOXO3 / FKHRL1 antibody

ELISA: FOXO3A Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

FOXO3 / FKHRL1 (361-460) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1F6 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

FOXO3 / FKHRL1 (361-460) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2H2 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

FOXO3 / FKHRL1 (361-460) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4E8 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

FOXO3 / FKHRL1 (361-460) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4F2 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

FOXO3 / FKHRL1 (361-460) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2C5 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

FOXO3 / FKHRL1 (361-460) antibody

Formalin-Fixed, Paraffin-Embedded Human Spleen stained FOXO3 / FKHRL1 Antibody Cat.-No AM31789PU-N at 5 µg/ml after heat-induced antigen retrieval. Mouse IgG2a 3C2 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

FOXO3 / FKHRL1 antibody

Western blot analysis of FOXO3a expression in lysate from Jurkat cells (Lane 1), Rat liver (Lane 2) and Rat kidney (Lane 3). Rabbit Aff - Purified Hu, Ms IP, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

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