FOXP3 antibody

Principal name

FOXP3 antibody

Alternative names for FOXP3 antibody

Forkhead box protein P3, IPEX, JM2, Scurfin

SwissProt ID

Q99JB6 (Mouse), Q9BZS1 (Human)

Gene ID

50943 (FOXP3), 20371 (Foxp3)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Por (Porcine), Rt (Rat), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep)

Available hosts

Goat, Rabbit, Mouse, Rat

Available applications

ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Flow Cytometry (F), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of FOXP3 antibody

Forkhead box protein P3 (Fox3p) is a probable transcription factor that plays a critical role in the control of immune response. Mice deficient in FOX3P (Scurfy mice) exhibited over proliferation of CD4+CD8- T lymphocytes, multi-organ infilteration and elevation of numerous cytokines. The Fox3P deficiency is lethal in hemizygous mice similar to CTLA4 or TGFbeta deficient mice, the pathology observed is in scurfy mice is similar to animals that lack expression of CTLA4 or TGF beta that result from inability to properly regulate CD4+CD8- T cell activity (1). strong correlation between expression of forkhead box P3 and ex vivo expression of CTLA-4 in Treg cells was observed. The protein encoded by scurfin gene, designated as FOX3P is a new member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved in humans and is essential for normal immune homeostasis. FoxP3 is a necessary transcription factor for the development and function of CD4+ regulatory T-cells (Tregs) and it inhibits HIV infection by inhibiting NFAT activity and NFAT proteins contribute to HIV-1 transcription (2). Mutations in FOX3P gene (homolog of mouse scurfy gene) resulted in IPEX (X-linked neonatal diabetes mellitus, enteropathy and endocrinopathy syndrome) disorder characterized by immune disorder characterized by immune dys-regulation, insulin dependent diabetes mellitus, infection, secretory diarrhea, thrombocytopenia, anemia and aczema. It is usually lethal in infancy. The CD4+CD25+ exert their suppressor function by regulating miR-142-3p via Adenylyl cyclase 9 (AC9) that inhibits AC9 production in Tregs. The FOX3P down-regulates miR-142-3p to keep the AC9-cAMP pathway active in Cd4+CD25+ Treg (3). The forkhead box p3 (Foxp3) positive Tregs have functionally immunosupressive properties that prevents effector cells from acting against self in autoimmune diseases/tumors are are highly relevant in cancer progression (4).
Fox3P is a soluble nuclear protein containing 431 amino acids (MW 52kDa) protein contains 1 C2H2-type zing finger and one forkhead DNA-binding domain. Fox3P is expressed in several splice variant forms. Epigenetic regulation of Fox3P locus contributes to its role as a lineage-specification factor for development of Tregs. The Fox3PP gene is located on chromosome Xp11.23-Xq13.3. Forkhead (FH), also known as a ’winged helix’, is named for the Drosophila fork head protein, a transcription factor which promotes terminal rather than segmental development. Disruption in Fox3pp/winged helix/scurfy protein led lethal lymphoproliferative disorder in scurfin mouse. The scurfin has several FH (forkhead DNA binding domains near C-terminal end of the protein. At least 2 variant isoforms are expressed, a longer iso-form a and a short iso-form b.

General readings

Chatila TA et al. JM2, encoding a fork head-related protein, is mutated in X-linked autoimmunity-allergic disregulation syndrome. J Clin Invest 106:R75-81 (2000). PubMed PMID: 11120765
1. Karube K et al. Expression of FoxP3, a key molecule in CD4CD25 regulatory T cells, in adult T-cell leukaemia/lymphoma cells. Br J Haematol. 126:81-4 (2004).
2. Nilsson, J., Boasso, A., Velilla, P. A., Zhang, R., Vaccari, M., Franchini, G., Shearer, G. M., Andersson, J., and Chougnet, C. (2006) HIV-1-driven regulatory T-cell accumulation in lymphoid tissues is associated with disease progression in HIV/AIDS, 108, 3808-3817.
3. Schmidt-Lucke, C., Aicher, A., Romagnani, P., Gareis, B., Romagnani, S., Dimmeler, S., and Zeiher, A. M. (2007) Specific recruitment of CD4+CD25++ regulatory T cells into the allograft in heart transplant recipients, 01197.02006.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FOXP3 antibody

Western Blot: FOXP3 Antibody [TA336306] - Detection of immunoprecipitated FOXP3 from HA-tagged FOXP3 transfected Jurkat T antigen cells. Rabbit Purified Hu, Ms C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FOXP3 (C-term) antibody

AM21067PU FOXP3 antibody staining of Human Tonsil. Rabbit IgG SP97 Purified Hu P
0.5 ml / €510.00
  Acris Antibodies GmbH

FOXP3 (C-term) antibody

AM21067PU FOXP3 antibody staining of Human Tonsil. Rabbit IgG SP97 Purified Bov, Can, Hu, Ms, Por, Rt P
1 ml / €730.00
  Acris Antibodies GmbH

FOXP3 (C-term) antibody

AM21067PU FOXP3 antibody staining of Human Tonsil. Rabbit IgG SP97 Purified Hu P
0.1 ml / €290.00
  Acris Antibodies GmbH

FOXP3 (C-term) antibody

AP15563PU FoxP3 antibody staining of Human Tonsil. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

FOXP3 (C-term) antibody

AP15563PU FoxP3 antibody staining of Human Tonsil. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

FOXP3 (C-term) antibody

AP15563PU FoxP3 antibody staining of Human Tonsil. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

FOXP3 antibody

Detection of human FOXP3. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3: 293/mouse foxp3, lane 4: 293/empty vector Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FOXP3 antibody

Detection of murine FOXP3. Lane 1: Human CD4+CD25+ PBL, lane 2: HEK293T transfected with human Foxp3 cDNA, lane 3: 293/mouse foxp3, lane 4: 293/empty vector Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FOXP3 (C-term) antibody

TA302662 (1µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Nuclear staining of scattered cells in the interfollicular area. B) High magnification of positive cells Goat Aff - Purified Can, Hu, Por ChIP, E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FOXP3 antibody

Western blot analysis of FOXP3 in A549 cell lysate with FOXP3 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FOXP3 antibody

Gel: 8%SDS-PAGE,Lysate: 40 µg,,Primary antibody: FOXP3 Antibody at dilution 1/222 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 2 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FOXP3 antibody

Western blot analysis of extracts from 293T(lane 1) and 293T transfected with mFOXP3(lane 2) using anti-FOXP3 antibody(poly6238). Rabbit IgG Poly6238 Purified Hu, Ms P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

FOXP3 antibody

Host: Rabbit; Target Name: Foxp3; Sample Tissue: Mouse Liver lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

FOXP3 antibody

WB Suggested Anti-FOXP3 Antibody Titration: 5.0-8.0ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FOXP3 antibody

Western Blot: FOXP3 Antibody (3G3) [TA336416] - analysis of FOXP3 in 1) full-length transfected and 2) mock transfected 293 cell lysate using this antibody. Mouse IgG1 3G3 Purified Hu, Ms F, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FOXP3 antibody

Western Blot: FOXP3 Delta 2 (exon 2 deleted) specific Antibody (16J4G6) [TA337092] - Analysis of FOXP3D2 and full-length (wild-type) transfected 293 cell lysate using TA337092 and FOXP3/Scurfin. At 1 ug/ml, TA337092 specifically detects the FOXP3D2 (Lane Mouse IgM 16J4G6 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FOXP3 antibody

Western blot detection of FOXP3 in CHO-K1 cell lysate(A)and CHO-K1 transfected by FOXP3(B)cell lysate using FOXP3 mouse mAb (1:300 diluted).Predicted band size:47KDa.Observed band size:50KDa. Mouse IgG1 7H9-D6-A10 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FOXP3 antibody

Western blot analysis using FOXP3 antibody Cat.-No AM06466SU-N against FOXP3(AA: 2-193)-hIgGFc transfected HEK293 cell. Mouse IgG1 4C7 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

FOXP3 antibody

Intracellular staining of human peripheral blood cells (gated on CD4+ cells) with anti-FoxP3 (clone 3G3). Mouse IgG1 3G3 Aff - Purified Hu, Ms F, WB
0.1 mg / €290.00
  Acris Antibodies GmbH

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