FTO antibody

Principal name

FTO antibody

Alternative names for FTO antibody

KIAA1752, Fat mass and obesity-associated protein

SwissProt ID

Q2A121 (Rat), Q8BGW1 (Mouse), Q9C0B1 (Human)

Gene ID

79068 (FTO), 26383 (Fto), 291905

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Eq (Equine), Prim (Primate), Ye (Yeast), GP (Guinea Pig)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of FTO antibody

FTO (alpha-ketoglutarate-dependent dioxygese FTO) is a novel protein belonging to FTO family and it is a nuclear dioxygese that repairs alkylated D and R by oxidative demethylation reactions. FTO exerts highest demethylase activity towards single-stranded R containing 3-methyluracil, followed by single-stranded D containing 3-methylthymine, low activity towards single-stranded D containing 1-methyladenine or 3-methylcytosine and is ictive towards 1-methylguanine or double-stranded D. FTO requires O2, alpha-ketoglutarate and iron (co-factor) for its activity and gets activated by ascorbate whereas its inhibitors include N-oxalylglycine, fumarate, succite etc. It contributes to the regulation of global metabolic rate, energy expenditure/homeostasis and has been suggested to control the body size as well as accumulation of body fat. FTO has been associated with various obesity traits, insulin resistance and NIDDM, and its defects are known to cause growth retardation developmental delay coarse facies and early death (GDFD).

General readings

Gerken T, Girard CA, Tung YC, Webby CJ, Saudek V, Hewitson KS, Yeo GS, McDonough MA, Cunliffe S, McNeill LA, Galvanovskis J, Rorsman P, Robins P, Prieur X, Coll AP, Ma M, Jovanovic Z, Farooqi IS, Sedgwick B, Barroso I, Lindahl T, Ponting CP, Ashcroft FM, O'Rahilly S, Schofield CJ.
The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase.
Science. 2007 Nov 30;318(5855):1469-72. Epub 2007 Nov 8.
PMID: 17991826

  1. Quick-Filters:
  2. Cited (1)
  3. Overnight Delivery (1)

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FTO (N-term) antibody

  Mouse Anti-FTO ( Fat mass and obesity related protein) Antibody  
Western blot of rat testes lysate showing specific immunolabeling of the ~ 58k Fto protein. Mouse IgG 5-2H10 Purified Bov, Can, Eq, Hu, Ms, non-Human Primates, Rt, Sh ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FTO antibody

  Goat Anti-FTO (Mouse) Antibody  
TA305858 (0.03ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FTO antibody

  Rabbit Polyclonal FTO Antibody  
Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FTO antibody

  Rabbit Polyclonal FTO Antibody  
Western Blot: FTO Antibody [TA336541] - Detection of FTO in human brain lysate using anti-FTO TA336541. Rabbit Purified Hu, Ms, Prim WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FTO antibody

  Rabbit Polyclonal Anti-Fto Antibody  
WB Suggested Anti-Fto Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Mouse Brain Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Sh, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

FTO antibody

  Rabbit Polyclonal Anti-FTO Antibody  
WB Suggested Anti-FTO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: PANC1 cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

FTO antibody

  Rabbit Polyclonal Anti-FTO Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Human fetal brain tissue, Primary antibody: (FTO Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FTO antibody

  Rabbit Polyclonal FTO Antibody  
Immunohistochemistry: FTO Antibody - A majority of the normal cells displayed moderate to strong cytoplasmic positivity. For example skeletal muscle cells and glandular cells of the gastrointestinal tract showed strong cytoplasmic immunoreactivity. Most of the malignant cells exhibited moderate cytoplasmic positivity. Strongly stained cases were mainly observed among colo-rectal, stomach, endometrial and pancreatic cancers. Malignant lymphomas and renal cancers were in several cases weakly stained or negative.  Image and statement courtesy of the Human Protein Atlas (HPA). Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FTO antibody

Western blot analysis using anti-FTO Antibody (human), mAb (FT86-4) at 1/2000 dilution and 100 ng protein.1: Human FTO 2: Mouse FTO 3: Rat FTO4: HEK 293 cell lysate (100 μg).5: Human Calreticulin (negative control). Mouse IgG1 FT86-4 Purified Hu, Ms, Rt WB
50 µl / €380.00
  Acris Antibodies GmbH

FTO (N-term) antibody

Western blot analysis of FTO Antibody (N-term) (AP17404PU-N) in NCI-H460 cell line lysates (35 µg/lane). FTO (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FTO (N/C-term) antibody

Endothelial (Lane 1) and epithelial (Lane 2) human cell lysates were analysed by SDS-PAGE and Western blotted using Rabbit anti-Human Fatso serum at 1/1000 followed by Goat anti-rabbit HRP. A single protein of approximately 60 kDa, the predicted size of full length Fatso, was detected on blots Rabbit Serum Hu E, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

FTO - human gene knockout kit via CRISPR

FTO - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

FTO - human gene knockout kit via CRISPR

FTO - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fto - mouse gene knockout kit via CRISPR

Fto - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Fto - mouse gene knockout kit via CRISPR

Fto - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fto - mouse gene knockout kit via CRISPR

Fto - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fto - mouse gene knockout kit via CRISPR

Fto - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

FTO

  Recombinant protein of human fat mass and obesity associated (FTO)  
FTO Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, FTO (mGFP-tagged) - Human fat mass and obesity associated (FTO), 200 uL, >10^7 TU/mL

Lenti ORF particles, FTO (mGFP-tagged) - Human fat mass and obesity associated (FTO), 200 uL, >10^7 TU/mL  
200 µl / €1,130.00
  OriGene Technologies, Inc.

Lenti ORF particles, FTO (Myc-DDK tagged) - Human fat mass and obesity associated (FTO) , 200 uL, >10^7 TU/mL

Lenti ORF particles, FTO (Myc-DDK tagged) - Human fat mass and obesity associated (FTO) , 200 uL, >10^7 TU/mL  
200 µl / €1,010.00
  OriGene Technologies, Inc.

Items 1 to 20 of 24 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn