FTO antibody

Principal name

FTO antibody

Alternative names for FTO antibody

KIAA1752, Fat mass and obesity-associated protein

SwissProt ID

Q2A121 (Rat), Q8BGW1 (Mouse), Q9C0B1 (Human)

Gene ID

79068 (FTO), 26383 (Fto)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Can (Canine), Por (Porcine), Rt (Rat), Bov (Bovine), Eq (Equine), Sh (Sheep), Rb (Rabbit), Prim (Primate), Ye (Yeast), GP (Guinea Pig)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Frozen Sections (C)

Background of FTO antibody

FTO (alpha-ketoglutarate-dependent dioxygese FTO) is a novel protein belonging to FTO family and it is a nuclear dioxygese that repairs alkylated D and R by oxidative demethylation reactions. FTO exerts highest demethylase activity towards single-stranded R containing 3-methyluracil, followed by single-stranded D containing 3-methylthymine, low activity towards single-stranded D containing 1-methyladenine or 3-methylcytosine and is ictive towards 1-methylguanine or double-stranded D. FTO requires O2, alpha-ketoglutarate and iron (co-factor) for its activity and gets activated by ascorbate whereas its inhibitors include N-oxalylglycine, fumarate, succite etc. It contributes to the regulation of global metabolic rate, energy expenditure/homeostasis and has been suggested to control the body size as well as accumulation of body fat. FTO has been associated with various obesity traits, insulin resistance and NIDDM, and its defects are known to cause growth retardation developmental delay coarse facies and early death (GDFD).

General readings

Gerken T, Girard CA, Tung YC, Webby CJ, Saudek V, Hewitson KS, Yeo GS, McDonough MA, Cunliffe S, McNeill LA, Galvanovskis J, Rorsman P, Robins P, Prieur X, Coll AP, Ma M, Jovanovic Z, Farooqi IS, Sedgwick B, Barroso I, Lindahl T, Ponting CP, Ashcroft FM, O'Rahilly S, Schofield CJ.
The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase.
Science. 2007 Nov 30;318(5855):1469-72. Epub 2007 Nov 8.
PMID: 17991826

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FTO (N-term) antibody

Western blot of rat testes lysate showing specific immunolabeling of the ~ 58k Fto protein. Mouse IgG 5-2H10 Purified Bov, Can, Eq, Hu, Ms, non-Human Primates, Rt, Sh ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FTO antibody

TA305858 (0.03ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FTO antibody

Western blot analysis of FTO in human uterus tissue lysate with FTO antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FTO antibody

Western Blot: FTO Antibody [TA336541] - Detection of FTO in human brain lysate using anti-FTO TA336541. Rabbit Purified Hu, Ms, Prim WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FTO antibody

WB Suggested Anti-Fto Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Mouse Brain Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Sh, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

FTO antibody

WB Suggested Anti-FTO Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: PANC1 cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

FTO antibody

Immunohistochemistry: FTO Antibody - A majority of the normal cells displayed moderate to strong cytoplasmic positivity. For example skeletal muscle cells and glandular cells of the gastrointestinal tract showed strong cytoplasmic immunoreactivity. Most of the malignant cells exhibited moderate cytoplasmic positivity. Strongly stained cases were mainly observed among colo-rectal, stomach, endometrial and pancreatic cancers. Malignant lymphomas and renal cancers were in several cases weakly stained or negative.  Image and statement courtesy of the Human Protein Atlas (HPA). Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FTO antibody

Western blot analysis using anti-FTO Antibody (human), mAb (FT86-4) at 1/2000 dilution and 100 ng protein.1: Human FTO 2: Mouse FTO 3: Rat FTO4: HEK 293 cell lysate (100 μg).5: Human Calreticulin (negative control). Mouse IgG1 FT86-4 Purified Hu, Ms, Rt WB
50 µl / €370.00
  Acris Antibodies GmbH

FTO (N-term) antibody

Western blot analysis of FTO Antibody (N-term) (AP17404PU-N) in NCI-H460 cell line lysates (35 µg/lane). FTO (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FTO Control Peptide

FTO
50 µg / €160.00
  Acris Antibodies GmbH

FTO (N/C-term) antibody

Endothelial (Lane 1) and epithelial (Lane 2) human cell lysates were analysed by SDS-PAGE and Western blotted using Rabbit anti-Human Fatso serum at 1/1000 followed by Goat anti-rabbit HRP. A single protein of approximately 60 kDa, the predicted size of full length Fatso, was detected on blots Rabbit Serum Hu E, WB
0.1 ml / €300.00
  Acris Antibodies GmbH

FTO antibody

Fto polyclonal antibody ( Cat # PAB11419 ) ( 0.03 µg/ml ) staining of human pancreas lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

FTO antibody

Western blot analysis of FTO in human brain lysate using FTO polyclonal antibody ( Cat # PAB12425 ). Rabbit Hu, Ms WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

FTO (N-term) antibody

FTO Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

FTO (Center) antibody

Human PANC1; WB Suggested Anti-FTO Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: PANC1 cell lysate; FTO antibody - middle region (ARP47474_P050) in Human PANC1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

FTO antibody

Western blot analysis of FTO expression in HepG2 (A); Caco2 (B); SHSY5Y (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rb, Rt, Sh WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

FTO antibody

Western blot analysis of FTO expression in HepG2 (A); Caco2 (B); SHSY5Y (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rb, Rt, Sh WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

FTO antibody

Western blot analysis of FTO expression in HepG2 (A); Caco2 (B); SHSY5Y (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rb, Rt, Sh WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

FTO antibody

Immunohistochemistry: FTO Antibody [23660002] - A majority of the normal cells displayed moderate to strong cytoplasmic positivity. For example skeletal muscle cells and glandular cells of the gastrointestinal tract showed strong cytoplasmic immunoreactivity. Most of the malignant cells exhibited moderate cytoplasmic positivity. Strongly stained cases were mainly observed among colo-rectal, stomach, endometrial and pancreatic cancers. Malignant lymphomas and renal cancers were in several cases weakly stained or negative.  Image and statement courtesy of the Human Protein Atlas (HPA). Rabbit Hu E, P
50 µg / €440.00
  Novus Biologicals Inc.

FTO antibody

FTO Rabbit Biotin Hu, Mky, Ms WB
0.1 ml / €360.00
  Novus Biologicals Inc.

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