Alternative names for FtsZ antibody
sfiB, sulB, b0095, JW0093, Cell division protein ftsZ
Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)
Background of FtsZ antibody
The bacterial cytokinetic protein FtsZ is a tubulin-like protein with GTPase activity and it plays an important role in cell division. The FtsZ protein is conserved throughout the bacteria and archaea where in response to an unknown cell division signal it polymerizes in to a ring which becomes the site of future cell division site. During cell division of B. subtilis, the frequency of cells with FtsZ rings varies 2-3-fold, however, the cellular concentration of FtsZ remain constant irrespective of doubling time (1). The FtsZ is expressed via a constitutive promoter thus eliminating the normal transcription al control. The FtsZ undergo GTP-dependent polymerization in to medial FtsZ ring-formation and separation at cell poles is mediated by several protein partners. The MinCD complex is found concentrated at the cell poles where it inhibits the FtsZ polymerization (2). The FtsZ protein has GTPase activity which correlated with the FtsZ polymerization and FtsZ ring formation in rapid times of approximately 10 seconds (3). GTPase dependent polymerizsation of FtsZ is inhibited by SulA which is further purified as a fusion portien to MalE and staphylococcus protein A. The FtsZ deficient B. subtilis mutants are deficient in the initiation stages of cell division and exhibit long filament like growth when subjected to non-permissive temperature. The free energy of GTP hydrolysis appears not to be used for generation o constriction forces thus the role of GTPase activity in ring formation is still an open question (4).
The Z ring is a very dynamic structure through which subunits of FtsZ cycle with a half time of about 10 seconds. FtsZ is a GTPase that is similar to the eukaryotic tubulins and is essential for cell division in prokaryotes. FtsZ is capable of polymerizing in a GTP-driven process into structures similar to those formed by tubulin. FtsZ forms a ring-shaped septum just prior to cell division. Staphylococcus aureus FtsZ is a 390 amino acid protein with a nucleotide binding site and a SulA interaction site. Disruption in the activity of FtsZ in S aureus affects its growth pattern and may be an important therapeutic target fight against staphylococcus infections.
- Cited (3)
|Rabbit||Aff - Purified||Bac||WB||
|Acris Antibodies GmbH|
|Acris Antibodies GmbH|
|Rabbit||Serum||Bac, Pl||ICC/IF, WB||
|Rabbit||Serum||Candidatus sp., Citrobacter sp. 30_2, Dickeya sp., E. coli, Enterobacter sp., Klebsiella pneumoniae subsp. pneumoniae MGH, Salmonella sp., Shigella sonnei Ss046, Vibrio sp., Yersinia pestis D182038||WB||
|+1 additional image|