FtsZ antibody

Principal name

FtsZ antibody

Alternative names for FtsZ antibody

sfiB, sulB, b0095, JW0093, Cell division protein ftsZ

SwissProt ID

P0A9A6 (Ecoli)

Gene ID

944786 (ftsZ)

Ncbi ID


Available reactivities

Bac (Bacteria), Pl (Plant), E. coli (Escherichia coli)

Available hosts


Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB)

Background of FtsZ antibody

The bacterial cytokinetic protein FtsZ is a tubulin-like protein with GTPase activity and it plays an important role in cell division. The FtsZ protein is conserved throughout the bacteria and archaea where in response to an unknown cell division signal it polymerizes in to a ring which becomes the site of future cell division site. During cell division of B. subtilis, the frequency of cells with FtsZ rings varies 2-3-fold, however, the cellular concentration of FtsZ remain constant irrespective of doubling time (1). The FtsZ is expressed via a constitutive promoter thus eliminating the normal transcription al control. The FtsZ undergo GTP-dependent polymerization in to medial FtsZ ring-formation and separation at cell poles is mediated by several protein partners. The MinCD complex is found concentrated at the cell poles where it inhibits the FtsZ polymerization (2). The FtsZ protein has GTPase activity which correlated with the FtsZ polymerization and FtsZ ring formation in rapid times of approximately 10 seconds (3). GTPase dependent polymerizsation of FtsZ is inhibited by SulA which is further purified as a fusion portien to MalE and staphylococcus protein A. The FtsZ deficient B. subtilis mutants are deficient in the initiation stages of cell division and exhibit long filament like growth when subjected to non-permissive temperature. The free energy of GTP hydrolysis appears not to be used for generation o constriction forces thus the role of GTPase activity in ring formation is still an open question (4).
The Z ring is a very dynamic structure through which subunits of FtsZ cycle with a half time of about 10 seconds. FtsZ is a GTPase that is similar to the eukaryotic tubulins and is essential for cell division in prokaryotes. FtsZ is capable of polymerizing in a GTP-driven process into structures similar to those formed by tubulin. FtsZ forms a ring-shaped septum just prior to cell division. Staphylococcus aureus FtsZ is a 390 amino acid protein with a nucleotide binding site and a SulA interaction site. Disruption in the activity of FtsZ in S aureus affects its growth pattern and may be an important therapeutic target fight against staphylococcus infections.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FtsZ (368-383) antibody

Western Blot of FtsZ Antibody Cat.-No AP10076PU-N Rabbit Aff - Purified Bac WB
0.1 mg / €420.00
  Acris Antibodies GmbH

FtsZ Control Peptide

0.25 mg / €250.00
  Acris Antibodies GmbH

FtsZ antibody

FtsZ Rabbit Serum Bac, Pl ICC/IF, WB
0.2 ml / €400.00
  AgriSera AB

FtsZ antibody

Total protein extract from E. coli CFT073: 8µg (1), 12µg (2), 16µg (3). Proteins were separated on 10% SDS-PAGE and blotted to PVDF. Blocked with 5 % non-fat milk in TBS-T for 1 hour. Blot was incubated in the primary antibody at a dilution of 1 :10 000 for 1 h at RT with agitation. Secondary antibody (anti-rabbit IgG, HRP conjugated, Agrisera, AS09 602) were diluted to 1 : 50 000 and blot was incubated for 1h at RT with agitation. Immunodetection was performed using ECL method for 3 min. Scan was made after 30 sec.
Courtesy of Dr. Marta Kicia, Wroclaw Medical University, Poland Rabbit Serum Candidatus sp., Citrobacter sp. 30_2, Dickeya sp., E. coli, Enterobacter sp., Klebsiella pneumoniae subsp. pneumoniae MGH, Salmonella sp., Shigella sonnei Ss046, Vibrio sp., Yersinia pestis D182038 WB
50 µl / €300.00
  AgriSera AB

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