Fumarase antibody

Principal name

Fumarase antibody

Alternative names for Fumarase antibody

HLRCC, LRCC, MCL, MCUL1, mitochondrial Fumarate hydratase

SwissProt ID

P07954 (Human), P10173 (Pig), P14408 (Rat), P97807 (Mouse)

Gene ID

2271 (FH)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Can (Canine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Dot blot (Dot), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (R), Flow Cytometry (F), Frozen Sections (C)

Background of Fumarase antibody

Fumarase (Fumarate hytdratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to S-malate and is involved in the tricarboxylic acid (TCA), or Krebs cycle. This enzyme exists in both a cytosolic form and an N-terminal extended mitochondrial form. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension is the same form as in the cytoplasm. Fumarase deficiency can lead to progressive encephalopathy, cerebral atrophy and developmental delay and this enzyme also is thought to act as a tumor suppressor. Recombinant Fumarase was expressed in E.coli and was purified by conventional chromatography techniques.

General readings

Pollard PJ, Spencer-Dene B, Shukla D, Howarth K, Nye E, El-Bahrawy M, Deheragoda M, Joannou M, McDonald S, Martin A, Igarashi P, Varsani-Brown S, Rosewell I, Poulsom R, Maxwell P, Stamp GW, Tomlinson IP. Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway. Cancer Cell. 2007 Apr;11(4):311-9. PMID: 17418408

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Fumarase antibody

Fumarate hydratase antibody [N2C2], Internal detects FH protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 10% SDS-PAGE. Fumarate hydratase antibody [N2C2], Internal (TA307962) dilution: 1:1000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Fumarase antibody

Fumarate hydratase antibody detects FH protein by Western blot analysis. A. 50 ug mouse liver lysate/extract. 7.5% SDS-PAGE. Fumarate hydratase antibody (TA307996) dilution: 1:1000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Fumarase antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500675) Mouse IgG1 OTI1F10 Purified Can, Hu, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Fumarase antibody

Immunofluorescent staining of HeLa cells using anti-FH mouse monoclonal antibody (TA500681). Mouse IgG1 OTI2A2 Purified Can, Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Fumarase antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500682) Mouse IgG1 OTI9G4 Purified Can, Hu, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Fumarase antibody

Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity with a granular pattern in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Fumarase antibody

Immunohistochemistry-Paraffin: Fumarase Antibody (1F10) [NBP1-47754] - Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FHmouse monoclonal antibody. (Dilution 1:50) Mouse IgG1 1F10 Aff - Purified Can, Hu, Rt F, ICC/IF, P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

Fumarase antibody

TA302493 (0.01µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Fumarase antibody

Western blot analysis of extracts of various cell lines, using FH antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Fumarase antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Fumarase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate
Lane 2. : LNCap cell lysate
Lane 3. : A549 cell lysate
Lane 4. : HepG2 cell lysate
Lane 5. : 293T cell lysate
Lane 6. : K562 cell lysate
Lane 7. : Jurkat cell lysate Mouse IgG2a AT3F6 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Fumarase antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Fumarase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate
Lane 2. : LNCap cell lysate
Lane 3. : A549 cell lysate
Lane 4. : HepG2 cell lysate
Lane 5. : 293T cell lysate
Lane 6. : K562 cell lysate
Lane 7. : Jurkat cell lysate Mouse IgG2a AT3F6 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

Fumarase antibody

Fumarase Rabbit IgG Azide Free Por Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €230.00
  Acris Antibodies GmbH

Fumarase antibody

Fumarase Rabbit IgG Biotin Por Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

Fumarase antibody

Fumarase Rabbit IgG Aff - Purified Por Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

Fumarase (176-189) antibody

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Can, Chk, Elephant, Eq, Fi, Hst, Hu, Insect, L, Mky, Ms, Op, Platypus, Por, Prim, Rt, Sh, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Fumarase antibody

Fumarase Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Fumarase antibody

FH monoclonal antibody (M01), clone 2E10. Western Blot analysis of FH expression in HeLa ( Cat # L013V1 ). Mouse IgG2b 2E10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Fumarase antibody

FH monoclonal antibody (M02), clone 5D8. Western Blot analysis of FH expression in HeLa ( Cat # L013V1 ). Mouse IgG2b 5D8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Fumarase antibody

Detection limit for recombinant GST tagged FH is approximately 1ng/ml as a capture antibody. Mouse IgG2b 2C5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Fumarase antibody

Detection limit for recombinant GST tagged FH is approximately 1ng/ml as a capture antibody. Mouse IgG1 5D4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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