Furin antibody

Principal name

Furin antibody

Alternative names for Furin antibody

FUR, PACE, PCSK3, Dibasic-processing enzyme, Paired basic amino acid residue cleaving enzyme

SwissProt ID

P09958 (Human), P23188 (Mouse), P23377 (Rat), Q28193 (Bovin)

Gene ID

5045 (FURIN), 18550 (Furin)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Sh (Sheep), Chimp (Chimpanzee), Eq (Equine), Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C)

Background of Furin antibody

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.

General readings

This product has been used in:Wang X & Pei D Shedding of membrane type matrix metalloproteinase 5 by a furin-type convertase: a potential mechanism for down-regulation. J Biol Chem 276:35953-60 (2001). PubMed PMID: 11470782
Liu G et al. Cytoskeletal protein ABP-280 directs the intracellular trafficking of furin and modulates proprotein processing in the endocytic pathway. J Cell Biol 139:1719-33 (1997). PubMed PMID: 9412467
General / background references:Shapiro J et al. Localization of endogenous furin in cultured cell lines. J Histochem Cytochem 45:3-12 (1997). PubMed PMID: 9010463
Molloy SS et al. Intracellular trafficking and activation of the furin proprotein convertase: localization to the TGN and recycling from the cell surface. EMBO J 13:18-33 (1994). PubMed PMID: 7508380
Steiner DF et al. The new enzymology of precursor processing endoproteases. J Biol Chem 267:23435-8 (1992). PubMed PMID: 1429684
Bresnahan PA et al. Human fur gene encodes a yeast KEX2-like endoprotease that cleaves pro-beta-NGF in vivo. J Cell Biol 111:2851-9 (1990). PubMed PMID: 2269657

20 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Furin antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human Furin with C-terminal MYC tag probed with AP32850PU-N (1 µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB08896 (1ug/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Furin antibody

Anti-FURIN antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Rabbit IgG Purified Can, Chimp, Eq, Hu, Mky, Ms, Rt
50 µg / €345.00
  OriGene Technologies, Inc.

Furin antibody

Western blot analysis of Furin in rat kidney tissue lysate. Rabbit Purified Bov, Hu, Ms, Rt, Sh WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Furin antibody

Western blot analysis of Furin expression in rat kidney tissue lysate. Rabbit Bov, Hu, Ms, Rt, Sh WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Furin antibody

Human THP-1; WB Suggested Anti-FURIN Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; FURIN antibody - N-terminal region (ARP45328_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Furin antibody

Figure 1 illustrates immunolocalization of endogenous furin in mouse 3T3 cells with NB100-1903. Rabbit Aff - Purified Can, Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Furin antibody

Western Blot: Furin Antibody [NBP1-45580] - Western blot analysis of Furin in rat kidney tissue lysate. Rabbit IgG Aff - Purified Bov, Hu, Ms, Rt, Sh WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Furin antibody

Furin Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Furin

Furin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Furin

Furin Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.

Regular Price: 10 µg / €199.00

Special Price: 10 µg / €139.00

  OriGene Technologies, Inc.

Furin

Furin Human Purified > 95 % pure by SDS-PAGE gel and HPLC analyses High-5 Insect cells
10 µg / €230.00
  Acris Antibodies GmbH

Furin

Furin Human Purified > 95 % pure by SDS-PAGE gel and HPLC analyses High-5 Insect cells
2 µg / €140.00
  Acris Antibodies GmbH

Furin (108-715, His-tag)

Furin Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Furin (108-715, His-tag)

Furin Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Furin

Furin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Furin

Furin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Furin

Furin Human
10 µg / €290.00
  Abnova Taiwan Corp.

ELISA Kits

Catalog No.    

FURIN (Human) ELISA Kit

FURIN (Human) ELISA Kit  
1 kit / €710.00
  Abnova Taiwan Corp.

Human Furin ELISA Kit

Human Furin ELISA Kit  
96 Tests / €460.00
  Boster Immunoleader

Human FURIN ELISA Kit

Representative Standard Curve  
96 Tests / €450.00
  Cohesion Biosciences Ltd.

20 Item(s)

per page
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