FYN antibody

Principal name

FYN antibody

Alternative names for FYN antibody

p59-Fyn, Protooncogene Syn, SLK, Proto-oncogene tyrosine-protein kinase Fyn

SwissProt ID

A0JNB0 (Bovin), A1Y2K1 (Pig), P06241 (Human), P13406 (Xenla), P39688 (Mouse), Q05876 (Chick), Q62844 (Rat)

Gene ID

2534 (FYN), 14360 (Fyn), 25150

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of FYN antibody

Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.The unique N terminal domain of Fyn interacts with the CD3 and eta chains of the TcR. Fyn can bind to other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of Fyn activity. Fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.

General readings

1. Utting O, Priatel JJ, Teh SJ, Teh HS: p59fyn (Fyn) promotes the survival of anergic CD4-CD8- alpha beta TCR+ cells but negatively regulates their proliferative response to antigen stimulation. J Immunol. 2001 Feb 1;166(3):1540-6.
2. Talmor-Cohen A, Tomashov-Matar R, Tsai WB, Kinsey WH, Shalgi R: Fyn kinase-tubulin interaction during meiosis of rat eggs. Reproduction. 2004 Oct;128(4):387-93.
3. Hattori K, Uchino S, Isosaka T, Maekawa M, Iyo M, Sato T, Kohsaka S, Yagi T, Yuasa S: Fyn is required for haloperidol-induced catalepsy in mice. J Biol Chem. 2006 Mar 17;281(11):7129-35.
4. Wu HY, Hsu FC, Gleichman AJ, Baconguis I, Coulter DA, Lynch DR: Fyn-mediated phosphorylation of NR2B Tyr-1336 controls calpain-mediated NR2B cleavage in neurons and heterologous systems. J Biol Chem. 2007 Jul 13;282 (28):20075-87.
5. Filby A, Seddon B, Kleczkowska J, Salmond R, Tomlinson P, Smida M, Lindquist JA, Schraven B, Zamoyska R. Fyn regulates the duration of TCR engagement needed for commitment to effector function. J Immunol. 2007 Oct 1;179(7):4635-44.
6. Samayawardhena LA, Kapur R, Craig AW: Involvement of Fyn kinase in Kit and integrin-mediated Rac activation, cytoskeletal reorganization, and chemotaxis of mast cells.
Blood. 2007 May 1;109(9):3679-86.
7. Makinodan E, Manabe T, Makinodan M, Yamauchi T, Matsuyoshi H, Sakumura R, Tatsumi K, Wanaka A. A novel role for Fyn: change in sphere formation ability in murine embryonic stem cells. Neuroscience. 2007 Jun 15;147(1):1-4.
8. Rivera J, Olivera A: A current understanding of Fc epsilon RI-dependent mast cell activation. Curr Allergy Asthma Rep. 2008 Mar;8(1):14-20.

  1. Quick-Filters:
  2. Highly Validated (3)

Items 1 to 20 of 43 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FYN antibody

  Rabbit polyclonal Anti-FYN Antibody  
WB Suggested Anti-FYN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

FYN antibody

Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using FYN mouse mAb with DAB staining. Mouse IgG1 2A10 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

FYN antibody

Figure 3. Immunofluorescence staining of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Mouse IgG2b FYN-01 Purified Hu, Ms ICC/IF, IP, P, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

FYN (N-term) antibody

  Goat Polyclonal Antibody against FYN  
TA302725 staining 0.2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FYN (N-term) antibody

  Rabbit polyclonal FYN Antibody (N-term)  
Western blot analysis of FYN Antibody (N-term) (Cat. #TA324974) in Hela cell line lysates (35ug/lane). FYN (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

FYN antibody

  Rabbit Polyclonal Fyn Antibody  
Western blot analysis of Fyn expression in 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FYN antibody

  Rabbit Polyclonal Fyn (Tyr530) Antibody (Phospho-specific)  
Western blot analysis of Fyn phosphorylation expression in H2O2 treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FYN antibody

  Rabbit polyclonal Fyn (Phospho-Tyr530) antibody  
Western blot analysis of extracts from LOVO cells, using Fyn (Phospho-Tyr530) antibody (#TA326057).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FYN antibody

  Rabbit polyclonal Fyn (Ab-530) antibody  
Western blot analysis of extracts from HUVEC cells, MCF-7 cells and 293 cells, using Fyn (Ab-530) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FYN antibody

  Rabbit polyclonal Anti-FYN Antibody  
WB Suggested Anti-FYN Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

FYN antibody

  Mouse Monoclonal Fyn Antibody  
Western blot detection of Fyn in Hela,3T3,C6,COS7,CEM,Ramos and Jurkat cell lysates using Fyn mouse mAb (1:500 diluted).Predicted band size:59KDa.Observed band size:59KDa. Mouse IgG2b 4B8-E7-A8 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FYN antibody

  Rabbit Polyclonal Anti-KYNU Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: A549, hela and 293T cell, Primary antibody: (FYN Antibody) at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FYN antibody

Figure 1. AM05300PU-N Fyn antibody staining of Formalin Fixed, Paraffin Embedded Human lymphoma tissue. Mouse IgG1 1S Purified Hu, Ms, Rt P, WB
0.1 mg / €580.00
  Acris Antibodies GmbH

FYN antibody

Western blot analysis using FYN mouse mAb against NIH/3T3 (1) and Hela (2) cell lysate. Mouse IgG1 2H8 Ascites Hu, Ms E, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

FYN (7-176) antibody

Formalin-Fixed, Paraffin- Embedded Human Brain Cerebellum stained with Fyn Antibody Cat.-No AM33106PU (Clone FYN-59) at 15 µg/ml after heat-induced antigen retrieval. Mouse IgG FYN-59 Aff - Purified Hu, Ms IP, P, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

FYN (7-176) antibody

Formalin-Fixed, Paraffin- Embedded Human Brain Cerebellum stained with Fyn Antibody Cat.-No AM33106PU (Clone FYN-59) at 15 µg/ml after heat-induced antigen retrieval. Mouse IgG FYN-59 Aff - Purified Hu, Ms IP, P, WB
25 µg / €220.00
  Acris Antibodies GmbH

FYN (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

FYN pTyr530 antibody

Immunohistochemistry analysis of Fyn antibody (Cat.-No.: AP20942PU-N) pTyr530 in paraffin-embedded Human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

FYN antibody

Western blot (WB) analysis of Fyn antibody (Cat.-No.: AP21058PU-N) in extracts from MCF-7 cells. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FYN pTyr530 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Fyn (Phospho-Tyr530) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €230.00
  Assay Biotech

Items 1 to 20 of 43 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn