FZD8 / Frizzled-8 antibody

Principal name

FZD8 / Frizzled-8 antibody

Alternative names for FZD8 / Frizzled-8 antibody

Fz-8, hFz8

SwissProt ID

O93274 (Xenla), Q498S8 (Rat), Q61091 (Mouse), Q9H461 (Human), Q9IA03 (Chick)

Gene ID

8325 (FZD8)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of FZD8 / Frizzled-8 antibody

FZD8 is a member of the frizzled family. Members of this family are seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of sigling proteins. Most frizzled receptors are coupled to the beta-catenin canonical sigling pathway. This protein is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of sigling proteins. Most frizzled receptors are coupled to the beta-catenin canonical sigling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.

General readings

Keay SK, Szekely Z, Conrads TP, Veenstra TD, Barchi JJ Jr, Zhang CO, Koch KR, Michejda CJ. An antiproliferative factor from interstitial cystitis patients is a frizzled 8 protein-related sialoglycopeptide. Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11803-8. Epub 2004 Jul 28. PMID: 15282374

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

FZD8 / Frizzled-8 (N-term) antibody

Anti-FZD8 / Frizzled 8 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Ms P
50 µg / €379.00
  OriGene Technologies, Inc.

FZD8 / Frizzled-8 (N-term) antibody

Anti-FZD8 / Frizzled 8 antibody IHC of human pancreas, islet of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Ms P
50 µg / €379.00
  OriGene Technologies, Inc.

FZD8 / Frizzled-8 (C-term) antibody

TA303076 (1µg/ml) staining of human pancreas lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

FZD8 / Frizzled-8 antibody

Western blot analysis of extracts from Jurkat cells, using FZD8 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

FZD8 / Frizzled-8 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and gastric cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using FZD8 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

FZD8 / Frizzled-8 antibody

Predicted cell location: Cytoplasm. Positive control: Human esophagus and liver cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using FZD8 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

FZD8 / Frizzled-8 (Extracell. Dom.) antibody

Anti-FZD8 / Frizzled 8 antibody IHC of human brain, cerebellum, Purkinje neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 8-20 ug/ml. Rabbit Aff - Purified Hu, Ms P
50 µg / €379.00
  OriGene Technologies, Inc.

FZD8 / Frizzled-8 antibody

WB Suggested Anti-FZD8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Can, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

FZD8 / Frizzled-8 antibody

Western blot (WB) analysis of Frizzled-8 Antibody Cat.-No. AP01267PU-N at 1/500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: sp2/0 whole cell lysate. Lane 3: H9C2 whole cell lysate. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

FZD8 / Frizzled-8 antibody

FZD8 / Frizzled-8 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

FZD8 / Frizzled-8 antibody

FZD8 polyclonal antibody ( Cat # PAB6916 ) ( 3 µg/ml ) staining of paraffin embedded human pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP- Goat Hu E, P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

FZD8 / Frizzled-8 antibody

Immunofluorescence analysis of A549 cells, using FZD8 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

FZD8 / Frizzled-8 antibody

Immunofluorescence analysis of A549 cells, using FZD8 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

FZD8 / Frizzled-8 antibody

Western blot analysis of extracts from Jurkat cells, using FZD8 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

FZD8 / Frizzled-8 antibody

Western blot analysis of extracts from Jurkat cells, using FZD8 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

FZD8 / Frizzled-8 antibody

Human MCF-7; WB Suggested Anti-FZD8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; FZD8 antibody - N-terminal region (ARP41283_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

FZD8 / Frizzled-8 antibody

Immunohistochemical analysis of Frizzled 8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

FZD8 / Frizzled-8 antibody

Immunohistochemical analysis of Frizzled 8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

FZD8 / Frizzled-8 antibody

Immunohistochemical analysis of Frizzled 8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

FZD8 / Frizzled-8 antibody

Immunohistochemistry: FZD8 Antibody [NB100-2439] - NB100-2439 (3ug/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP- Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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