G-CSF antibody

Principal name

G-CSF antibody

Alternative names for G-CSF antibody

CSF3, GCSF, Pluripoietin, C17orf33, Granulocyte colony-stimulating factor, Colony stimulating factor 3

SwissProt ID

O02708 (Felca), O02837 (Pig), P09919 (Human), P09920 (Mouse), P35833 (Bovin), P35834 (Canfa), Q28746 (Sheep)

Gene ID

1440 (CSF3), 12985 (Csf3)

Available reactivities

Hu (Human), Ms (Mouse), Eq (Equine), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat, Chicken

Available applications

Flow Cytometry (F), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Immunoprecipitation (IP)

Background of G-CSF antibody

Granulocyte colony stimulating factor (G-CSF) is the primary extracellular regulator of granulopoiesis and regulates the production of neutrophils by stimulating proliferation and survival of specific bone marrow precursor cells and their differentiation into granulocytes. Neutrophils play a critical role in the defence against bacterial and fungal infections. G-CSF is produced by monocytes, macrophages, neutrophils, fibroblasts and endothelial cells and is capable of increasing the absolute number of circulating neutrophils and enhancing their antimicrobial function. Unlike GMCSF, the activity of G-CSF is not species specific. Additionally, G-CSF production is inducible by cytokines including TNF-alpha, IL-1, GM-CSF, IL-17 and IL-4. Granulocyte colony stimulating factor (G-CSF) is a glycoprotein consisting of 207 amino acids. Differential splicing of G-CSF mRNA can result in two protein variants either 177- or 180-amino acids in length. G-CSF contains 5 cysteine residues, four of which form intermolecular disulfide bonds. There is a potential O-glycosylation site at Thr-166, which inhibits aggregation and stabilises the molecule. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

Roberts AW (2005) Growth Factors 23: 33-41.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

G-CSF antibody

Western blot analysis of G-CSF using recombinant human G-CSF. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human esophagus cancer and tonsil tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSF3 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

To detect hG-CSF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

To detect hG-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (TA328321) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

To detect mG-CSF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mG-CSF. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

To detect mG-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine G-CSF (TA328514) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mG-CSF. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF3 (RC217237, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF3. Mouse IgG1 OTI1G8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

G-CSF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF3 (RC207709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF3.(1:2000) Mouse IgG1 OTI4H9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

G-CSF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF3 (RC207709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF3. Mouse IgG1 OTI7B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

G-CSF antibody

G-CSF Rat IgG1 BVD13-3A5 Purified Hu E
0.5 mg / €440.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rat IgG2a BVD11-37G10 Biotin Hu E
0.5 mg / €540.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rat IgG2a BVD11-37G10 Purified Hu E, WB
0.5 mg / €440.00
  Acris Antibodies GmbH

G-CSF antibody

Direct ELISA using G-CSF Antibody Cat.-No PP022B Rabbit Biotin Ms E, WB
25 µg / €230.00
  Acris Antibodies GmbH

G-CSF antibody

Direct ELISA using G-CSF Antibody Cat.-No PP022B Rabbit Biotin Ms E, WB
50 µg / €340.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rabbit Aff - Purified Ms E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rabbit Aff - Purified Ms E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

G-CSF antibody

Immunohistochemistry: This G-CSF antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

G-CSF antibody

Immunohistochemistry: This G-CSF antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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