G-CSF antibody

Principal name

G-CSF antibody

Alternative names for G-CSF antibody

CSF3, GCSF, Pluripoietin, C17orf33, Granulocyte colony-stimulating factor, Colony stimulating factor 3

SwissProt ID

O02708 (Felca), O02837 (Pig), P09919 (Human), P09920 (Mouse), P35833 (Bovin), P35834 (Canfa), Q28746 (Sheep)

Gene ID

12985 (Csf3), 1440 (CSF3)

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat)

Available hosts

Rabbit, Rat, Mouse, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Functional assay (FN), Paraffin Sections (P), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Immunoprecipitation (IP)

Background of G-CSF antibody

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced byactivated monocytes/macrophages, fibroblasts, endothelial cells, astrocytes, osteoblasts and bone marrow stromal cells. In addition, various carcinoma cell lines and myeloblastic leukemia cells can produce G-CSF constitutively. G-CSF and G-CSF receptors have also been shown to be produced by placental, decidual and endometrial gland cells during pregnancy, suggesting that G-CSF may play a role in decidual and placental functions . Clinical use of G-CSF has been approved for the amelioration of chemotherapy-induced neutropenia as well as for severe chronic neutropenia following bone marrow transplants. Several reviews on G-CSF and its clinical applications have been published .

In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid (aa) residue precursor proteins, have been isolated. Both proteins have a 30 aa signal peptide and have identical sequences except for a three amino acid insertion (deletion) at the 35th aa residue from theN-terminus of the mature protein. The 177 aa residue G-CSF, derived from the 207 aa residue precursor, was reported to have a ten fold lower specific activity than the shorter G-CSF isoform. Human G-CSF is 73% identical at the amino acid level to mouse G-CSF and the two proteins show species cross-reactivity .

G-CSF exerts its biological effects through binding to specific cell surface receptors. A single class of high affinity binding sites is present in both human and mouse hematopoietic progenitors and neutrophilic granulocytes. Among nonhematopoietic cells, receptors are also expressed on placentalcells, endothelial cells, and various carcinoma cell lines. cDNAs for the human and mouse G-CSF receptors have been isolated . Mature mouse or human G-CSF receptors are single transmembrane glycoproteins of approximately 800 aa residues. The extracellular domain of the G-CSF receptor contains three regions of homology with other cell surface proteins, including an N-terminal immunoglobulin-like region, a structural motif characteristic of the hematopoietin receptor superfamily, and three tandem repeats of the fibronectin type III domain. In humans, four alternative transcripts of the G-CSF receptor gene, encoding a soluble form of G-CSF receptor as well as three transmembrane receptor isoforms have been identified .

General readings

Saito, S. et al. (1994) Growth Factors 10:135.

Nagata, S. (1990) in Peptide Growth Factors and Their Receptors, Sporn, M.B. and A.B. Roberts eds., Springer-Verleg, New York, p. 190.

3. Gabrilove, J.L. (1992) Growth Factors 6:187.

Nagata, S. (1994) in The Cytokine Handbook, 2nd ed., Academic Press, A. Thomson ed., New York, p. 371.

Felser, J.M. (1994) Ann. NY Acad. Sci. 730:235.

Harmenberg, J. et al. (1994) Eur. J. Haematol. Suppl. 55:1.

Fukunaga, R. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8702.

Fukunaga, R. et al. (1990) J. Biol. Chem. 265:14008.

Fukunaga, R. et al. (1990) Cell 61:341.

Larsen, A. et al. (1990) J. Exp. Med. 172:1559.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

G-CSF antibody

Western blot analysis of G-CSF using recombinant human G-CSF. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human esophagus cancer and tonsil tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSF3 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

To detect hG-CSF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

To detect hG-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (TA328321) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hG-CSF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

To detect mG-CSF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mG-CSF. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

To detect mG-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine G-CSF (TA328514) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mG-CSF. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

G-CSF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF3 (RC217237, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF3. Mouse IgG1 OTI1G8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

G-CSF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF3 (RC207709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF3.(1:2000) Mouse IgG1 OTI4H9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

G-CSF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF3 (RC207709, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF3. Mouse IgG1 OTI7B3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

G-CSF antibody

G-CSF Rat IgG1 BVD13-3A5 Purified Hu E
0.5 mg / €440.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rat IgG2a BVD11-37G10 Biotin Hu E
0.5 mg / €540.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rat IgG2a BVD11-37G10 Purified Hu E, WB
0.5 mg / €440.00
  Acris Antibodies GmbH

G-CSF antibody

Direct ELISA using G-CSF Antibody Cat.-No PP022B Rabbit Biotin Ms E, WB
25 µg / €230.00
  Acris Antibodies GmbH

G-CSF antibody

Direct ELISA using G-CSF Antibody Cat.-No PP022B Rabbit Biotin Ms E, WB
50 µg / €340.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rabbit Aff - Purified Ms E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rabbit Aff - Purified Ms E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

G-CSF antibody

G-CSF Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

G-CSF antibody

Immunohistochemistry: This G-CSF antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

G-CSF antibody

Immunohistochemistry: This G-CSF antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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