Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), Ze (Zebrafish)
Mouse, Rabbit, Goat
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)
Background of G protein alpha Q / GNAQ antibody
Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta (MIM 600230).[supplied by OMIM]
Mudhar,H.S, et al. (2013) Br J Ophthalmol 97 (7), 924-928Chishiki,K., et al. (2013) Biochem. Biophys. Res. Commun. 435 (3), 414-419
Western Blot analysis of GNAQ expression in transfected 293T cell line (H00002776-T02) by GNAQ MaxPab polyclonal antibody.Lane 1: GNAQ transfected lysate(39.49 KDa).Lane 2: Non-transfected lysate.
Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti GNAQ/Gα q Polyclonal Antibody, Unconjugated (bs-6152R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 42kD. Observed band size: 42kD.
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-GNAQ/Gα q Polyclonal Antibody, Unconjugated (bs-6152R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.