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GABRA1 antibody

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Principal name

GABRA1 antibody

Alternative names for GABRA1 antibody

GABRA-1, GABA A receptor subunit alpha-1, Gamma-aminobutyric acid receptor subunit alpha-1

SwissProt ID

P08219 (Bovin), P14867 (Human), P19150 (Chick), P62812 (Mouse), P62813 (Rat)

Gene ID

2554 (GABRA1), 29705 (Gabra1), 14394

Ncbi ID

NP_000797, NP_899155.1, NP_000797.2, 9606, 10116, NM_001127643, NM_001127644, NM_001127645, NM_001127648, NP_034380.1, NM_010250, NM_183326, NM_000806, NM_001127646, NM_001127647

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Mky (Monkey), Ze (Zebrafish), African clawed frog, Por (Porcine), Rb (Rabbit), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Immunoprecipitation (IP)

Background of GABRA1 antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces.
There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2).
The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (3). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (4).

General readings

Peran,M., et al., (2004) Neurosci. Lett. 364 (2), 67-70

ARP34976_P050 - GABRA1
Human HepG2; WB Suggested Anti-GABRA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; GABRA1 antibody - N-terminal region (ARP34976_P050) in Human HepG2 cells using Western Blot
PAB9624 - GABRA1
Western blot of mouse forebrain lysates from Wild Type (Control, lane 1) and Gabra1 knockout (Gabra1-K/O, lane 2) animals showing specific immunolabeling of the ~51 a1-subunit of the Gabra1. The labeling was absent from a lysate prepared from Gabra1 knockout animals.
ARP34977_P050 - GABRA1
Human 721_B; WB Suggested Anti-GABRA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 721_B cell lysate; GABRA1 antibody - middle region (ARP34977_P050) in Human 721_B cells using Western Blot
AP20674PU-N - GABRA1
Immunohistochemistry (IHC) analyzes of GABAA Ralpha1 antibody (Cat.-No.: AP20674PU-N) in paraffin-embedded human brain tissue.
AP54963PU-N - GABRA1
Brain stained in IHC-P with GABRA1 antibody
CPA3225-100ul - GABRA1
Western blot analysis of GABRA1 expression in U87MG (A); NIH3T3 (B) whole cell lysates.
75-136 - GABRA1
adult rat brain immunohistochemistry (with antigen retrieval via pepsin pretreatment)
75-136 - GABRA1
adult rat brain membrane immunoblot
75-136 - GABRA1
immunoblot of adult mouse membranes from wild-type (WT) and GABA-A-R-Alpha1 knockout (KO) mice probed with N95/35 (green) and anti-actin polyclonal (red). Image courtesy of Dominique Arion, Kelly Rogers and David Lewis (University of Pittsburgh).
73-136 - GABRA1
adult rat brain immunohistochemistry (with antigen retrieval via pepsin pretreatment)
73-136 - GABRA1
adult rat brain membrane immunoblot
73-136 - GABRA1
immunoblot of adult mouse membranes from wild-type (WT) and GABA-A-R-Alpha1 knockout (KO) mice probed with N95/35 (green) and anti-actin polyclonal (red). Image courtesy of Dominique Arion, Kelly Rogers and David Lewis (University of Pittsburgh).
TA309123 - GABRA1
Western blot of mouse forebrain lysates from wild type (Control) and α1-knockout (α1-K/O) animals showing specific immunolabeling of the ~51k α1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α1-knockout animals.
TA309124 - GABRA1
Western blot of mouse forebrain lysates from Wild Type (Control) and α1-knockout (α1-K/O) animals showing specific immunolabeling of the ~51k α1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α1-knockout animals.
TA326472 - GABRA1
Western blot analysis of GAB(A)R, Delta in a human cell line mix using a 1:1000 dilution of the antibody
TA328809 - GABRA1
Western blot analysis of rat brain lysates: 1. Anti-GABA(A) a1 Receptor (extracellular) antibody, (1:1000). 2. Anti-GABA(A) a1 Receptor (extracellular) antibody preincubated with the control peptide antigen.
TA328809 - GABRA1
Expression of GABA(A) a1 Receptor in mouse hippocampus. Immunohistochemical staining of GABA(A) a1 Receptor in mouse hippocampus using Anti-GABA(A) a1 Receptor (extracellular) antibody . A. Distribution of GABA(A) a1 Receptor (red) in mouse hippocampus. B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that distribution of GABA (A) α1 Receptor is restricted to neurons and their processes. DAPI is used as the counterstain (blue).
TA328809 - GABRA1
IHC staining of rat cerebellum using Anti-CaV1.2 antibody and Anti-GABA(A) a1 Receptor (extracellular) antibody. A. CaV1.2 (green) is detected in the granule layer of the cerebellum (G) and in the upper molecular layer (star). B. In the same section, GABA(A) a1 Receptor (red) is seen in the granule layer. C. Merge of the two images reveals high degree of colocalization between CaV1.2 and GABA(A) a1 Receptor in the granule layer.
TA338499 - GABRA1
WB Suggested Anti-GABRA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate
TA338500 - GABRA1
WB Suggested Anti-GABRA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 721_B cell lysate
TA351205 - GABRA1
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: LoVo cells, Primary antibody: (GABRA1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
TA351205 - GABRA1
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (GABRA1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA351205 - GABRA1
The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using (GABRA1 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200)
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GABRA1 antibody
  Rabbit Anti-GABAA Receptor a 1 Antibody  
Western blot of mouse forebrain lysates from wild type (Control) and α1-knockout (α1-K/O) animals showing specific immunolabeling of the ~51k α1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α1-knockout animals. Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, non-Human Primates, Rt Dot, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
 

Published recently!

GABRA1 (N-term) antibody
  Rabbit Anti-GABAA Receptor a 1, N-Terminus Antibody  
Western blot of mouse forebrain lysates from Wild Type (Control) and α1-knockout (α1-K/O) animals showing specific immunolabeling of the ~51k α1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α1-knockout animals. Rabbit IgG Aff - Purified Bov, Can, Hu, Ms, non-Human Primates, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GABRA1 (327-450) antibody
  Mouse Monoclonal Anti-GABA(A) Receptor alpha1 Antibody  
Western blot analysis of GAB(A)R, Delta in a human cell line mix using a 1:1000 dilution of the antibody Mouse IgG2a S95-35 Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GABRA1 antibody
  Rabbit Polyclonal Anti-GABA (A) alpha1 Receptor (extracellular)  
Western blot analysis of rat brain lysates: 1. Anti-GABA(A) a1 Receptor (extracellular) antibody, (1:1000). 2. Anti-GABA(A) a1 Receptor (extracellular) antibody preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.
+2 additional images

GABRA1 antibody
  Rabbit Polyclonal Anti-GABRA1 Antibody  
WB Suggested Anti-GABRA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GABRA1 antibody
  Rabbit Polyclonal Anti-GABRA1 Antibody  
WB Suggested Anti-GABRA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 721_B cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GABRA1 antibody
  Rabbit Polyclonal Anti-GABRA1 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: LoVo cells, Primary antibody: (GABRA1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+2 additional images

GABRA1 antibody
adult rat brain immunohistochemistry (with antigen retrieval via pepsin pretreatment) Mouse IgG2a N95/35 Supernatant Hu, Ms, Rt C, ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated
+2 additional images

NeuroMab

GABRA1 antibody
adult rat brain immunohistochemistry (with antigen retrieval via pepsin pretreatment) Mouse IgG2a N95/35 Purified Hu, Ms, Rt C, ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated
+2 additional images

NeuroMab

GABRA1 antibody
GABRA1 Rabbit IgG Purified Bov, Can, Chk, Hu, Mky, Ms, Rt WB
0.1 ml / €500.00
  Acris Antibodies GmbH

GABRA1 (N-term) antibody
GABRA1 Rabbit IgG Purified Ms, Rt WB
0.1 ml / €510.00
  Acris Antibodies GmbH

GABRA1 antibody
Immunohistochemistry (IHC) analyzes of GABAA Ralpha1 antibody (Cat.-No.: AP20674PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

GABRA1 (alpha-1) antibody
Brain stained in IHC-P with GABRA1 antibody Rabbit Purified Hu, Ms, Rt C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

GABRA1 (N-term) antibody
GABRA1 Rabbit IgG Serum Hu, Rt WB
0.1 ml / €410.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

GABRA1 - human gene knockout kit via CRISPR
GABRA1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GABRA1 - human gene knockout kit via CRISPR
GABRA1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GABRA1 - human gene knockout kit via CRISPR
GABRA1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GABRA1 - human gene knockout kit via CRISPR
GABRA1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gabra1 - mouse gene knockout kit via CRISPR
Gabra1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Gabra1 - mouse gene knockout kit via CRISPR
Gabra1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

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