GABRA5 antibody

Principal name

GABRA5 antibody

Alternative names for GABRA5 antibody

GABA A receptor subunit alpha-5, GABRA-5, Gamma-aminobutyric acid receptor subunit alpha-5

SwissProt ID

P19969 (Rat), P31644 (Human), Q08E50 (Bovin), Q8BHJ7 (Mouse)

Gene ID

29707 (Gabra5), 2558 (GABRA5), 110886

Available reactivities

Rt (Rat), Ms (Mouse), Bov (Bovine), Chk (Chicken), Hu (Human), Can (Canine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of GABRA5 antibody

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six Alpha's, four Beta's and four Gamma's, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for Alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a Gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different Alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

General readings

1. Brandon NJ, Jovanovic JN, Colledge M, Kittler JT, Brandon JM, Scott JD, Moss SJ (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor B-subunits. Mol Cell Neurosci 22:87-97.
2. McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor a1-subtype. Nature Neurosci 3:587-592.
3. Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing a1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
4. Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor g2-subunit. Biochem Pharmacol 68:1621-1629.
5. Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.
6. Pöltl A, Hauer B, Fuchs K, Tretter V, Sieghart W (2003) Subunit composition and quantitative importance of GABAA
receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455.
7. Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellčs B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653. 1. Maison, S. F., Rosahl, T. W., Homanics, G. E., and Liberman, M. C. (2006) Functional Role of GABAergic Innervation of the Cochlea: Phenotypic Analysis of Mice Lacking GABAA Receptor Subunits {alpha}1, {alpha}2, {alpha}5, {alpha}6, beta2, beta3, or {delta}, 26, 10315-10326.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GABRA5 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GABRA5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806473) Dilution: 1:150 Mouse IgG2b OTI1G9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GABRA5 antibody

Western blot of 7 μg of rat hippocampus (Hipp) showing specific immunolabeling of the ~55k α5-subunit of the GABAA-R. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt, Ze WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GABRA5 antibody

WB Suggested Anti-GABRA5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GABRA5 antibody

WB Suggested Anti-GABRA5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

GABRA5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GABRA5 (RC216541, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GABRA5. Mouse IgG1 OTI1C2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GABRA5 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GABRA5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA806596)(1:150) Mouse IgG2b OTI3H7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GABRA5 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-GABRA5 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806598) Dilution: 1:150 Mouse IgG1 OTI1G5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GABRA5 (C-term) antibody

GABRA5 Rabbit IgG Purified Ms, Rt WB
0.1 ml / €500.00
  Acris Antibodies GmbH

GABRA5 antibody

GABRA5 Rabbit Bov, Chk, Hu, Ms, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

GABRA5 (Center) antibody

Mouse Liver; WB Suggested Anti-Gabra5 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse liver; Gabra5 antibody - middle region (ARP30687_P050) in Mouse Liver cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

GABRA5 antibody

Human Heart; WB Suggested Anti-GABRA5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; GABRA5 antibody - middle region (ARP34983_P050) in Human Heart cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

GABRA5 antibody

mouse bulbus; GABRA5 antibody - middle region (ARP34984_P050) in mouse bulbus cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

GABRA5 antibody

Western Blot: GABA A receptor alpha 5 subunit Antibody [NB300-195] - Western blot detection of the ~55kDa a5-subunit of GABAa-R from 7ug of rat hippocampal lysate using NB300-195. Rabbit Purified Rt WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GABRA5

GABRA5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

GABRA5

GABRA5 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

GABRA5

GABRA5 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

GABRA5

GABRA5 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

GABRA5

GABRA5 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

GABRA5

GABRA5 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GABRA5 overexpression lysate

GABRA5 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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