GAD1 / GAD67 antibody

Principal name

GAD1 / GAD67 antibody

Alternative names for GAD1 / GAD67 antibody

Glutamate decarboxylase 1, GAD-67, Glutamate decarboxylase 67 kDa isoform, 67 kDa glutamic acid decarboxylase

SwissProt ID

A0PA85 (Canfa), P14748 (Felca), P18088 (Rat), P48318 (Mouse), P48319 (Pig), Q0VCA1 (Bovin), Q99259 (Human)

Gene ID

24379 (Gad1), 2571 (GAD1), 14415

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Fe (Feline), Bov (Bovine), Can (Canine), Por (Porcine), Bt (Bat), Eq (Equine), Op (Opossum), Mky (Monkey), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse, Chicken

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of GAD1 / GAD67 antibody

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L glutamate to g-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. GAD has a restricted tissue distribution. It is highly expressed in the cytoplasm of GABAergic neurons in the central nervous system (CNS) and pancreatic beta cells. It is also present in other non-neuronal tissues such as testis, oviduct and ovary. GAD is also transiently expressed in non-GABAergic cells of the embryonic and adult nervous system, suggesting its involvement in development and plasticity. GAD exists as two isoforms, GAD65 and GAD67 (molecular masses of 65 and 67 kD, respectively) that are encoded by two different genes. GAD65 is an ampiphilic, membraneanchored protein, (585 amino acid residues) and is encoded on human chromosome 10. GAD67 is a cytoplasmic protein (594 amino acid residues) and is encoded on chromosome 2. There is 64% amino acid identity between the two isoforms, with the highest diversity located at the N terminus, which in GAD65 is required for targeting the enzyme to GABA-containing secretory vesicles. The two isoforms appear to have distinct intraneuronal distribution in the brain. GAD65 has been identified as an autoantigen in insulindependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS), IDDM is an autoimmune disease that results from T cell mediated destruction of pancreatic insulin-secreting beta cells. Islet-reactive T cells and primarily to GAD65 (also named beta cell autoantigen) can be detected in peripheral blood of 80% of recent-onset IDD patients and in pre-diabetic high-risk subjects before onset of clinical symptoms. This suggests that GAD may be an important marker in the early stages of the disease. Also, auto to GAD65 and GAD67 are detected in animal models of IDDM, including the non-obese diabetes (NOD) mouse. In the NOD mouse, T cell reactivity is initially restricted to the C terminal regions of GAD65, but later spreads to other parts of GAD65. Stiff-man syndrome (SMS), a rare disorder of the CNS, is characterized by progressive rigidity of the body musculature with painful spasms, due to impairment of the GABAergic neurotransmission.

General readings

1. Huang HS, Akbarian S.
GAD1 mRNA expression and DNA methylation in prefrontal cortex of subjects with schizophrenia.
PLoS ONE 2007 2 (8): e809.
PMID: 17726539

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GAD1/2 antibody

Immunohistochemical analysis of GAD1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

GAD1/2 antibody

Immunohistochemical analysis of GAD1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

GAD1/2 antibody

Immunohistochemical analysis of GAD1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

GAD1 / GAD67 antibody

GAD67 antibody detects GAD67 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. GAD67 antibody (TA308881) dilution: 1:500 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500328) Mouse IgG2a OTI3H2 Purified Can, Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GAD1 / GAD67 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500329) Mouse IgG2b OTI3G9 Purified Hu, Mky ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

GAD1 / GAD67 antibody

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500330, Dilution 1:50) Mouse IgG1 OTI5D8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GAD1 / GAD67 (+ GAD2 / GAD65) antibody

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GAD1 / GAD67 antibody

Immunohistochemistry-Paraffin: GAD67 Antibody (3G9) [NBP1-48272] - Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-GAD1mouse monoclonal antibody. (Dilution 1:50) Mouse IgG2b 3G9 Aff - Purified Hu, Mky ICC/IF, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

GAD1 / GAD67 (+GAD25) antibody

This antibody (AP31572PU-N)(1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

GAD1 / GAD67 antibody

TA305623 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

Western blot analysis of extracts of fetal brain cell line, using GAD1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

WB Suggested Anti-Gad1 Antibody Titration: 1 ug/ml; Positive Control: Mouse Liver lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

WB Suggested Anti-GAD1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

Western blot analysis on LOVO cell lysate using GAD1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

Western blot analysis on mouse brain cell lysate using GAD1/2 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 (1-585) antibody

Immunohistochemical detection of GAD on formalin fixed paraffin embedded section of human pancreas with AM26579AF-N. Mouse IgG1 9A6 Azide Free Hu, Ms, Rt P, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

GAD1 / GAD67 antibody

Immunohistochemistry analysis of GAD67 antibody (Cat.-No.: AP06130PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GAD1 / GAD67 antibody

Immunohistochemistry (IHC) analyzes of GAD1/2 antibody (Cat.-No.: AP06389PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GAD1 / GAD67 (1-100) antibody

Testis: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

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