GAD1 / GAD67 antibody

Principal name

GAD1 / GAD67 antibody

Alternative names for GAD1 / GAD67 antibody

Glutamate decarboxylase 1, GAD-67, Glutamate decarboxylase 67 kDa isoform, 67 kDa glutamic acid decarboxylase

SwissProt ID

A0PA85 (Canfa), P14748 (Felca), P18088 (Rat), P48318 (Mouse), P48319 (Pig), Q0VCA1 (Bovin), Q99259 (Human)

Gene ID

24379 (Gad1), 2571 (GAD1), 14415

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Fe (Feline), Bt (Bat), Bov (Bovine), Can (Canine), Eq (Equine), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Rb (Rabbit), Bear, GP (Guinea Pig)

Available hosts

Goat, Rabbit, Mouse, Chicken

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of GAD1 / GAD67 antibody

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (γ-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

General readings

1. Huang HS, Akbarian S.
GAD1 mRNA expression and DNA methylation in prefrontal cortex of subjects with schizophrenia.
PLoS ONE 2007 2 (8): e809.
PMID: 17726539

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GAD1/2 antibody

Immunohistochemical analysis of GAD1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

GAD1/2 antibody

Immunohistochemical analysis of GAD1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

GAD1/2 antibody

Immunohistochemical analysis of GAD1/2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

GAD1 / GAD67 antibody

GAD67 antibody detects GAD67 protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 7.5 % SDS-PAGE. GAD67 antibody (TA308881) dilution: 1:500 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500328) Mouse IgG2a OTI3H2 Purified Can, Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GAD1 / GAD67 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500329) Mouse IgG2b OTI3G9 Purified Hu, Mky ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

GAD1 / GAD67 antibody

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500330, Dilution 1:50) Mouse IgG1 OTI5D8 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GAD1 / GAD67 (+ GAD2 / GAD65) antibody

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GAD1 / GAD67 (+GAD25) antibody

This antibody (AP31572PU-N)(1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GAD1 / GAD67 antibody

TA305623 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

  Rabbit polyclonal anti-GAD67/GAD1 antibody  
Western blot analysis of extracts from LOVO cells, using GAD67 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

Western blot analysis of extracts of fetal brain cell line, using GAD1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

WB Suggested Anti-Gad1 Antibody Titration: 1 ug/ml; Positive Control: Mouse Liver lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

WB Suggested Anti-GAD1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

Western blot analysis on LOVO cell lysate using GAD1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

Western blot analysis on mouse brain cell lysate using GAD1/2 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 antibody

GAD1 / GAD67 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAD1 / GAD67 (1-585) antibody

Immunohistochemical detection of GAD on formalin fixed paraffin embedded section of human pancreas with AM26579AF-N. Mouse IgG1 9A6 Azide Free Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

GAD1 / GAD67 antibody

Immunohistochemistry analysis of GAD67 antibody (Cat.-No.: AP06130PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GAD1 / GAD67 antibody

Immunohistochemistry (IHC) analyzes of GAD1/2 antibody (Cat.-No.: AP06389PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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