GADD153 / CHOP antibody

Principal name

GADD153 / CHOP antibody

Alternative names for GADD153 / CHOP antibody

DDIT3, DDIT-3, CHOP10, CHOP-10, Growth arrest and DNA-damage-inducible protein GADD153, C/EBP-homologous protein, C/EBP-homologous protein 10, DNA damage-inducible transcript 3

SwissProt ID

P14607 (Crigr), P35638 (Human), P35639 (Mouse), Q0IIB6 (Bovin), Q62857 (Rat)

Gene ID

1649 (DDIT3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus), Mky (Monkey), Por (Porcine), Bov (Bovine), Sh (Sheep), Prim (Primate)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of GADD153 / CHOP antibody

CHOP (also known as growth arrest and DNA-damage-inducible protein GADD153) is a 27 kD member of the C/EBP transcription factor family containing a basic region involved in DNA binding and a leucine zipper domain. This nuclear protein functions as a dimer to inhibit the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA target sequences. CHOP (GADD153) is induced by endoplasmic-reticulum stress and nutrient deprivation. Phosphorylation by p38-MAP kinase enhances activity of this protein while CK2 phosphorylation decreases activity. CHOP (GADD153) has been shown to interact with c-jun, c-fos, C/EBP, and LAP. 
Structure: C/EBP transcription factor family, basic region involved in DNA binding, leucine zipper domain, dimer; 27 kD.
Distribution: Nuclear.
Function: Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA target sequences.
Regulation: Induced by endoplasmic-reticulum stress and nutrient deprivation. Phosphorylation by p38-MAP kinase enhances activity. CK2 phosphorylation decreases activity.
Modification: Phosphorylation.
Interaction: c-jun, c-fos, C/EBP, LAP

General readings

General / background references:Wang XZ et al. Cloning of mammalian Ire1 reveals diversity in the ER stress responses. EMBO J 17:5708-17 (1998). PubMed PMID: 9755171
Wang XZ et al. Identification of novel stress-induced genes downstream of chop. EMBO J 17:3619-30 (1998). PubMed PMID: 9649432
Batchvarova N et al. Inhibition of adipogenesis by the stress-induced protein CHOP (Gadd153). EMBO J 14:4654-61 (1995). PubMed PMID: 7588595

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GADD153 / CHOP antibody

GADD153 / CHOP Rabbit Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

GADD153 / CHOP antibody

Antibody was tested using uninduced E. coli cell lysates (lane 1) or E. coli cell lysates induced with IPTG (lane 2) to express CHOP. Total lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anto-CHOP antibody (Poly6436) antibody. The proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Rabbit IgG Aff - Purified Hu WB
25 µl / €220.00
  Acris Antibodies GmbH

GADD153 / CHOP antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDIT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA802218) Mouse IgG2a OTI2D3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GADD153 / CHOP antibody

Western Blot: CHOP/GADD153 Antibody (9C8) - Figure 1 shows a Western blot of endogenous CHOP/GADD153 from primary human fibroblasts using anti-CHOP.  Lane 1: Untreated cells, Lane 2: Cells treated with tunicamycin for 10 hours. Mouse IgG2b 9C8 Purified Hu, Ms C, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GADD153 / CHOP (C-term) antibody

DDIT3 Antibody (C-term A135) (Cat. #TA324451) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms F, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GADD153 / CHOP (Center) antibody

DDIT3 Antibody (Center) (Cat. #TA324507) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the DDIT3 antibody detected the DDIT3 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GADD153 / CHOP antibody

Western blot analysis of CHOP expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GADD153 / CHOP antibody

Western blot analysis of extracts from mouse craniofacial tissue , using CHOP antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

GADD153 / CHOP antibody

Western Blot: CHOP/GADD153 Antibody [TA336900] - CHOP levels following treatment in human neuroblastoma. Image from verified customer review. Rabbit Purified Hu, Ms, Prim, Rt C, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GADD153 / CHOP antibody

Western blot analysis on Jurkat cell lysate using GADD153 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GADD153 / CHOP antibody

Western blot analysis of CHOP expression in Jurkat cell lysate Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GADD153 / CHOP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDIT3 (RC201301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDIT3. Mouse IgG1 OTI4G2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GADD153 / CHOP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDIT3 (RC201301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDIT3. Mouse IgG2a OTI4C4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GADD153 / CHOP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDIT3 (RC201301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDIT3. Mouse IgG1 OTIH7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GADD153 / CHOP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDIT3 (RC201301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDIT3. Mouse IgG1 OTIE7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GADD153 / CHOP Control Peptide

GADD153 / CHOP Purified
50 µg / €190.00
  Acris Antibodies GmbH

GADD153 / CHOP antibody

Immunohistochemistry (IHC) analysis of GADD153 / CHOP Antibody Cat.-No AP06131PU-N in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GADD153 / CHOP antibody

Western blot (WB) analysis of CHOP antibody (Cat.-No.: AP06513PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GADD153 / CHOP antibody

Human Kidney (formalin-fixed, paraffin-embedded) stained with DDIT3 antibody AP22879PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GADD153 / CHOP pSer30 antibody

Western blot analysis of extracts from Jurkat cells treated with PMA using Phospho-CHOP (Ser30) antibody AP55925PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €340.00
  Acris Antibodies GmbH

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