GADD45A antibody

Principal name

GADD45A antibody

Alternative names for GADD45A antibody

GADD45, DDIT1, DDIT-1, Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein

SwissProt ID

P24522 (Human), P48316 (Mouse), P48317 (Rat), Q3ZBN6 (Bovin), Q60GI5 (Felca)

Gene ID

1647 (GADD45A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)

Background of GADD45A antibody

Growth arrest and DNA-damage-inducible protein GADD45 alpha (GADD45A) is a protein that in humans is encoded by the GADD45A gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. Papathanasiou et al. (1991) demonstrated that the GADD45 gene is located on 1p34-p12. Sequence analysis of human and hamster cDNA clones demonstrated that the gene has been highly conserved and encodes a novel 165-amino acid polypeptide. Northern blot analysis detected moderate expression of a 1.4-kb GADD45A transcript in heart, skeletal muscle, and kidney, with little or no expression in brain, placenta, lung, liver, and pancreas. Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GADD45A antibody

Western blot analysis of extracts of various cell lines, using GADD45A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

GADD45A antibody

WB Suggested Anti-GADD45A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human heart Rabbit IgG Purified Bov, Eq, Hu, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

GADD45A antibody

Western blot detection of GADD45 a in CHO-K1 cell lysate(B)and CHO-K1 transfected by GADD45 a His fusion protein(A)cell lysate using GADD45 a mouse mAb (1:1000 diluted). Predicted band size:22KDa. Observed band size:22KDa. Mouse IgG1 6C3-G3-E9 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GADD45A antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (RC204005, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A. Mouse IgG2b OTI1C9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GADD45A antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45A (RC204005, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD45A. Mouse IgG1 OTI2B7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GADD45A (N-term) antibody

Western blot analysis of anti-GADD45A Antibody (N-term) (Cat.#AP12434PU-N) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

GADD45A (C-term) antibody

Western blot analysis of GADD45A Antibody (C-term) (AP18028PU-N) in Y79 cell line lysates (35ug/lane). GADD45A (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GADD45A antibody

GADD45A Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

GADD45A antibody

Western Blot analysis of GADD45A expression in transfected 293T cell line (H00001647-T01) by GADD45A MaxPab polyclonal antibody.

Lane 1: GADD45A transfected lysate(18.30 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GADD45A antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP2K6 and GADD45A HeLa cells were stained with anti-MAP2K6 rabbit purified polyclonal 1:1200 and anti-GADD45A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 3D12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GADD45A (C-term) antibody

Human Heart; WB Suggested Anti-GADD45A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human heart; GADD45A antibody - C-terminal region (ARP60167_P050) in Human Heart cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

GADD45A antibody

GADD45A Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

GADD45A antibody

GADD45A Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GADD45A

GADD45A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

GADD45A

GADD45A Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

GADD45A (1-165, His-tag)

GADD45A Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

GADD45A (1-165, His-tag)

GADD45A Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

GADD45A

GADD45A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

GADD45A

GADD45A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

GADD45A

GADD45A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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