GAK antibody

Principal name

GAK antibody

Alternative names for GAK antibody

Cyclin G-associated kinase

SwissProt ID

O14976 (Human), P97874 (Rat), Q99KY4 (Mouse)

Gene ID

2580 (GAK)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of GAK antibody

he cell cycle of eukaryotes is regulated by cyclins and cyclin-dependent protein kinases (CDKs). Cyclin G1 exhibits closest sequence identity to the cyclin A family. Identification of cyclin G1 as a direct transcriptional target of the p53 tumor suppressor gene product indicates that cyclin G functions downstream of p53. GAK (a cyclin G associated kinase), an association partner of cyclin G1 and CDK5, which is expressed ubiquitously, with the highest level of expression being observed in the testis. The predicted amino acid sequence of GAK possesses an N-terminal Ser/Thr protein kinase catalytic domain with a long C-terminal extension. There are two putative dinucleotide-binding motifs in the kinase domain suggesting that GAK activity is some how modulated by dinucleotides such as ADP or GDP. As GAK lacks PSTAIR motif, it can not be considered a member of the CDK family. Kinase activities of GAK is almost throughout the cell cycle. In the middle part of the molecule, GAK resembles both tensin, an actin-binding component of adhesion plaques (also called focal contacts) and other submembranous cyto skeletal structure, and auxilin, a coat component of brain clathrin-coated vesicles. The domain homologous to tensin and auxilin occupies more than 50% of the whole molecule. A leucine zipper motif, whose physiological meaning is unknown, also located in this domain. A phosphorylation recognition site for a tyrosine kinase is located at position 403-410 (KGDLDISY). One, 19 and 24 potential phosphorylation sites for cAMP-dependent protein kinase, protein kinase C and casein kinase II protein kinase, respectively, are also located through whole mol ecule. The presence of a Ser/Thr kinase domain at the N-terminal portion of the molecule suggests that GAK plays a role as a modulator of signal transduction, by relaying the phosphorylation signal from an unidentified tyrosine kinase to unidentified GAK phosphorylation targets.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GAK antibody

Western blot analysis of extracts from 293 cells, using GAK antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAK antibody

Western blot analysis on 293 cell lysate using GAK Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAK (1-1305) antibody

Western blot analysis of GAK expression in HeLa (1), HL-60 (2) and L5178Y (3) using AM26606AF-N. Mouse IgG1 1C2 Azide Free Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

GAK (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GAK (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GAK (N-term) antibody

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GAK antibody

Western Blot analysis of GAK expression in transfected 293T cell line (H00002580-T01) by GAK MaxPab polyclonal antibody.

Lane 1: GAK transfected lysate(45.76 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GAK antibody

GAK MaxPab rabbit polyclonal antibody. Western Blot analysis of GAK expression in mouse testis. Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

GAK antibody

GAK MaxPab rabbit polyclonal antibody. Western Blot analysis of GAK expression in mouse testis. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

GAK antibody

GAK monoclonal antibody (M01), clone 4C10 Western Blot analysis of GAK expression in HeLa NE ( Cat # L013V3 ). Mouse IgG1 4C10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GAK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with GAK polyclonal antibody ( Cat # PAB3266 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

GAK antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with GAK polyclonal antibody ( Cat # PAB3267 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

GAK antibody

Western blot analysis of extracts from 293 cells, using GAK Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

GAK antibody

Western blot analysis of extracts from 293 cells, using GAK Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

GAK antibody

GAK Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

GAK antibody

GAK Rabbit IgG Aff - Purified Hu, Ms E, WB
50 µg / €290.00
  Novus Biologicals Inc.

GAK antibody

Immunohistochemistry-Paraffin: GAK Antibody [NBP1-89441] - Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GAK

GAK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

GAK

GAK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GAK

GAK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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