Galectin-9 antibody

Principal name

Galectin-9 antibody

Alternative names for Galectin-9 antibody

LGALS9, Galectin9, HOM-HD-21, Ecalectin

SwissProt ID

O00182 (Human), O08573 (Mouse), P97840 (Rat), Q3MHZ8 (Bovin)

Gene ID

3965 (LGALS9), 16859 (Lgals9)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (capture) (E(capture)), Flow Cytometry (F)

Background of Galectin-9 antibody

Galectin-9 is a mammalian lectin with a molecular weight around 40 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates.  HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis; galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
Structure: 355 amino acids; 40 kD.
Distribution: Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells.
Function: Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells.
Ligand Receptor: HAVCR2/TIM3.

General readings

1. Jiang J  et al. Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis in gastric cancer. PLoS One 8:e81799 (2013). IHC-P ; Human . PubMed: 24339967
2. Alam S  et al. Galectin-9 expression in endothelial cells is positively regulated by histone deacetylase 3. J Biol Chem : (2011). WB, ICC/IF ; Human . PubMed: 22027828

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Galectin-9 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA805650) Dilution: 1:150 Mouse IgG1 OTI19F7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Galectin-9 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805651) Mouse IgG1 OTI19H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Galectin-9 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805655) Mouse IgG2a OTI2A4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Galectin-9 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806323) Mouse IgG2b OTI1D12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Galectin-9 antibody

Western blot analysis of Galectin-9 expression in Jurkat cell lysate (Lane 1, 2). Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Galectin-9 antibody

Host: Rabbit; Target Name: LGALS9; Sample Tissue: HCT15 Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Galectin-9 antibody

WB Suggested Anti-LGALS9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Galectin-9 antibody

Western blot analysis of LEG9 expression in HepG2 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Galectin-9 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS9 (RC210750, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS9. Mouse IgG2a OTI1G3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Galectin-9 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805615) Mouse IgG2a OTI8B11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Galectin-9 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805627) Mouse IgG2a OTI21B5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Galectin-9 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS9 (RC210750, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS9.(1:2000) Mouse IgG2b OTI16B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Galectin-9 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS9 (RC210750, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS9. Mouse IgG1 OTI2E10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Galectin-9 antibody

Ecalectin ELISA analysis. Plates are coated with the capture antibody AM26514AF-N and are added the recombinant humen Ecalectin and are measured by the biotinylated detector antibody. Mouse IgG1 ECA8 Azide Free Hu E(capture), F
0.1 mg / €390.00
  Acris Antibodies GmbH

Galectin-9 antibody

Flow cytometric analysis of Ecalectin expression on Jurkat cells. Open histogram indicates the reaction of Isotypic control to the cells. Shaded histogram indicates the reaction of AM26515AF-N to the cells. Mouse IgG2b ECA42 Azide Free Hu F
0.1 mg / €390.00
  Acris Antibodies GmbH

Galectin-9 antibody

Galectin-9 Mouse IgG2b B-T33 Azide Free Hu E, F
0.2 mg / €270.00
  Acris Antibodies GmbH

Galectin-9 antibody

Galectin-9 Mouse IgG2b B-T33 Purified Hu F
2ml (200T) / €250.00
  Acris Antibodies GmbH

Galectin-9 antibody

Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Galectin-9 Antibody (clone 9S2-1). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 9S2-1 Purified Hu WB
0.1 mg / €360.00
  Acris Antibodies GmbH

Galectin-9 antibody

Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Galectin-9 Antibody (clone 9S2-1). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Mouse IgG1 9S2-1 Purified Hu WB
25 µg / €240.00
  Acris Antibodies GmbH

Galectin-9 antibody

Galectin-9 Mouse IgG1 B-K38 Azide Free Hu E
0.2 mg / €270.00
  Acris Antibodies GmbH

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