GAP43 antibody

Principal name

GAP43 antibody

Alternative names for GAP43 antibody

Neuromodulin, Axonal membrane protein GAP-43, Growth-associated protein 43, PP46, Neural phosphoprotein B-50

SwissProt ID

P06836 (Bovin), P06837 (Mouse), P07936 (Rat), P17677 (Human), P35001 (Chick)

Gene ID

2596 (GAP43), 29423 (Gap43), 14432

Available reactivities

Rt (Rat), Mam (Mammalia), Fe (Feline), Hu (Human), Mky (Monkey), Ms (Mouse), Chk (Chicken), Bov (Bovine), Can (Canine), Xen (Xenopus), Ze (Zebrafish), Snake, Rb (Rabbit), Prim (Primate)

Available hosts

Rabbit, Mouse, Sheep, Chicken

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of GAP43 antibody

GAP43 (growth associated protein 43, also called B-50, PP46, calmodulin-binding protein P-57, neuromodulin, neuron growth-associated protein 43, protein F1) is a major component of motile "growth cones" that form the tips of elongating axons as well as the syptic plasma membranes. GAP43 is associated with nerve growth process and plays a key role in axol as well as dendritic filopodia induction. It has been detected in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN, and binds calmodulin with superior affinity in the absence of Ca2+ than in its presence. Intracellular Ca2+ regulates PKC mediated phosphorylation of GAP43 and affects GAP43's ability to bind calmodulin. GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functiol cerebral cortex. Aberrant expression of GAP43 has been seen in patients diagnosed with schizophrenia and Alzheimer's disease. Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one med GAP43. The HGNC me for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 was independently discovered by several different groups and therefore has several alterte mes, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual SDS-PAGE mobility properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa (to perform such calculations yourself see this link). GAP43 is one of many highly negatively charged extended molecules which lack well defined tertiary structure and contain few hydrophobic residues and which run anomalously on SDS-PAGE. Other examples are CAP23, MARCKS, microtubule associated proteins MAP2, tau and the Neurofilament subunits. GAP43 has been extensively studied and is known to be a major protein kise C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.

General readings

Dent E W., et al. (1998) J Neurobiol. 35:287-299.Neve R L., et al. (1998) J Neurosci. 18: 7757-7767.

  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (1)

Items 1 to 20 of 54 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GAP43 pSer41 antibody

Figure 1. Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Bov, Can, Chk, Finch, Hu, Ms, non-Human Primates, Rt, Xen, Ze C, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

GAP43 antibody

Western Blot: GAP43 Antibody [TA336612] - Western blots of homogenate of cow cerebellum stained with RPCA-GAP43 . A prominent band running at ~43kDa represents the full length GAP43. Rabbit Purified Chk, Hu, Ms, Prim, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAP43 antibody

Western Blot: GAP43 Antibody - Western blot analysis of GAP43 in human brain protein using anti-GAP43. Sheep IgG Purified Can, Fe, Hu, Ms, Prim, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAP43 antibody

Immunohistochemical analysis of paraffin embedded Rat tissue sections (brain) using GAP43 antibody Mouse IgG2a GAP-8A12 Purified Chk, Hu, Ms, Rt, Snake C, ICC/IF, IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

GAP43 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GAP43 antibody AP02772PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

GAP43 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using GAP43 antibody AP02772PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

GAP43 pSer41 antibody

GAP43 Rabbit IgG Purified Bov, Can, Chk, Hu, Mky, Ms, Rt, Xen, Ze WB
0.1 ml / €510.00
  Acris Antibodies GmbH

GAP43 pSer41 antibody

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using GAP43 (Phospho-Ser41) Antibody (#AP09476PU, Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
0.1 ml / €320.00
  Acris Antibodies GmbH

GAP43 pSer41 antibody

Figure 1. Immunofluorescence staining of methanol-fixed HeLa cells using GAP43 (Phospho-Ser41) Antibody (#AP09476PU, Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
50 µl / €240.00
  Acris Antibodies GmbH

GAP43 (216-226) antibody

GAP43 Rabbit IgG Liquid Chk, Mam C, ICC/IF, P, WB
0.2 ml / €340.00
  Acris Antibodies GmbH

GAP43 antibody

Immunohistochemistry (IHC) analyzes of GAP43 antibody (Cat.-No.: AP20750PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

GAP43 pSer41 antibody

GAP43 Rabbit Aff - Purified Hu, Ms, Rt ICC/IF
0.1 mg / €350.00
  Acris Antibodies GmbH

GAP43 (C-term) antibody

Immunohistochemical analysis of Paraffin-Embedded Rat Tissue Brain Sections using GAP43 antibody Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

GAP43 (C-term) antibody

Immunochemical staining of mixed neuron/glial cultures showing GAP43 (green) labeling of numerous axonal and dendritic profiles and anti-alpha II spectrin (red). Chicken Ig Fraction Hu, Ms, Rt ICC/IF, WB
0.1 ml / €340.00
  Acris Antibodies GmbH

GAP43 (C-term) antibody

GAP43 staining of cultured cortical neurons purified from the neonatal Mouse brain (green satining).
 Red staining corresponds to a Rabbit anti- Alpha III Spectrin. 
Blue staining corresponds to the Hoechst nuclear satin. Chicken Ig Aff - Purified Ms ICC/IF, P, WB
0.2 ml / €290.00
  Acris Antibodies GmbH

GAP43 antibody

GAP43 Rabbit Serum Rt C, ICC/IF
50 µl / €550.00
  Acris Antibodies GmbH

GAP43 antibody

GAP43 Rabbit Serum Mam C, IP, WB
0.1 ml / €330.00
  Acris Antibodies GmbH

GAP43 antibody

GAP43 MaxPab polyclonal antibody. Western Blot analysis of GAP43 expression in rat brain. Mouse Serum Hu, Rt E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

GAP43 antibody

GAP43 MaxPab polyclonal antibody. Western Blot analysis of GAP43 expression in rat brain. Mouse Purified Hu, Rt E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GAP43 antibody

Western Blot analysis of GAP43 expression in transfected 293T cell line (H00002596-T02) by GAP43 MaxPab polyclonal antibody.

Lane 1: GAP43 transfected lysate(24.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Items 1 to 20 of 54 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn