GAPDH antibody

Principal name

GAPDH antibody

Alternative names for GAPDH antibody

GAPD, CDABP0047, Glyceraldehyde-3-Phosphate Dehydrogenase

SwissProt ID

P00355 (Pig), P00356 (Chick), P00360 (Yeast), P04406 (Human), P04797 (Rat), P10096 (Bovin), P16858 (Mouse), P46406 (Rabit), Q28259 (Canfa), Q28554 (Sheep), Q5XJ10, Q9N2D5 (Felca)

Gene ID

2597 (GAPDH), 24383 (Gapdh), 100009074, 853395 (TDH1), 100042025 (LOC100042025), 14433

Available reactivities

Bov (Bovine), Hu (Human), Fe (Feline), Gt (Goat), Ms (Mouse), Por (Porcine), Rt (Rat), Amph (Amphibian), Av (Birds), Mam (Mammalia), Can (Canine), Rb (Rabbit), Fi (Fish), Chk (Chicken), Dros (Drosophila), Mky (Monkey), Prim (Primate), Xen (Xenopus), Bakers Yeast, E. coli (Escherichia coli), Frog, Ye (Yeast), Bac (Bacteria), Insect, Pr (Protozoa), Hst (Hamster), Sh (Sheep)

Available hosts

Mouse, Rabbit, Chicken, Goat, Sheep

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Frozen Sections (C), Dot blot (Dot), Immunodiffusion (ID), Immunoprecipitation (IP), Radioimmunoassay (R), EPair for Elisa (EPair), Flow Cytometry (F), ChIP assay (ChIP)

Selected product citations ‐ of ACR001P

1. Kadow S, Jux B, Zahner SP, Wingerath B, Chmill S, Clausen BE, et al. Aryl hydrocarbon receptor is critical for homeostasis of invariant gammadelta T cells in the murine epidermis. J Immunol. 2011 Sep 15;187(6):3104-10. doi: 10.4049/jimmunol.1100912. Epub 2011 Aug 15. PubMed PMID: 21844385.
2. Jux B, Kadow S, Esser C. Langerhans cell maturation and contact hypersensitivity are impaired in aryl hydrocarbon receptor-null mice. J Immunol. 2009 Jun 1;182(11):6709-17. doi: 10.4049/jimmunol.0713344. PubMed PMID: 19454665.
3. Salameh A, Krautblatter S, Baessler S, Karl S, Rojas Gomez D, Dhein S, et al. Signal transduction and transcriptional control of cardiac connexin43 up-regulation after alpha 1-adrenoceptor stimulation. J Pharmacol Exp Ther. 2008 Jul;326(1):315-22. doi: 10.1124/jpet.108.136663. Epub 2008 Apr 29. PubMed PMID: 18445782.
4. Wachi T, Cornell B, Marshall C, Zhukarev V, Baas PW, Toyo-Oka K. Ablation of the 14-3-3gamma Protein Results in Neuronal Migration Delay and Morphological Defects in the Developing Cerebral Cortex. Dev Neurobiol. 2015 Aug 22. doi: 10.1002/dneu.22335. PubMed PMID: 26297819.
5. Columbus DA, Steinhoff-Wagner J, Suryawan A, Nguyen HV, Hernandez-Garcia A, Fiorotto ML, et al. Impact of prolonged leucine supplementation on protein synthesis and lean growth in neonatal pigs. Am J Physiol Endocrinol Metab. 2015 Sep 15;309(6):E601-10. doi: 10.1152/ajpendo.00089.2015. Epub 2015 Aug 4. PubMed PMID: 26374843. 
6. Menzel, L;Kleber, L;Friedrich, C;Hummel, R;Dangel, L;Winter, J;Schmitz, K;Tegeder, I;Schäfer, MK. Progranulin protects against exaggerated axonal injury and astrogliosis following traumatic brain injury.Glia 2016. PubMed PMID: 27778404.

Background of GAPDH antibody

Glyceraldehyde-3-phosphate dehydrogenase (GPDH) is a glycocytic enzyme which catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) (1). This enzyme exists as a tetramer of identical chains. It is found on a cell’s membrane, in the cytoplasm and in the nucleus, therefore, it has been shown to display several different activities. It functions in endocytosis, microtubule bundling, phosphotransfer activity, nuclear tRNA export, DNA replication and DNA repair (2). Recently, GPDH has been shown to play a significant role in the development of diabetic retinopathy and its progression after cessation of hyperglycemia (3). GPDH is also known as an initiation signal for cellular apoptosis or programmed cell death (PCD). Since PCD has shown to play some role in both chronic and acute human neurodegenerative disorders, GAPDH may serve as a target for anti-apoptotic compounds with the potential to slow or prevent the progression of neurodegenerative disorders (4).
PCD was shown to be initiated after the small scale translocation of GPDH in the nucleus that acts as a transcription factor for the synthesis of new GPDH protein. This protein is targeted for nucleus and has the characteristics of a novel isozyme that acts as a transcription factor for pro-apoptotic signals (5). Therefore, increase in nuclear GPDH is associated with susceptible neurons in postmortem samples from patients with a variety of neurodegenerative conditions. Small-molecule compounds that selectively bind GAPDH prevent the increase in GPDH synthesis and nuclear accumulation, hence preventing the cells from undergoing apoptosis (6). Such compounds are being considered as disease-modifying agents for neurodegenerative diseases. GPDH is approximately a 39kDa protein (333 amino acids).

General readings

Tarze A, Deniaud A, et al. (2007). Oncogene. 26(18):2606-20.Tisdale EJ, et al. (2007). Traffic. 8(6):733-41.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GAPDH antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GAPDH monoclonal antibody. Mouse IgG1 OTI2D9 Purified Can, Hu, Mky, Ms, Rt WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

TrueMAB

GAPDH (Loading Control) antibody

Staining of Rh30 rhabdomyosarcoma (upper panels) and SJSA-1 Ewing’s sarcoma  cells (lower panels) for GAPDH with ACR001P anti-GAPDH monoclonal antibody (left panels).  FITC conjugated secondary antibody. The middle panels show DAPI staining of the cell nuclei. The right panels show merged images. Mouse IgG1 6C5 Purified Can, Fe, Fi, Hu, Ms, Por, Rb, Rt E, ICC/IF, IP, WB
0.2 mg / €270.00
  Acris Antibodies GmbH

GAPDH (Loading Control) antibody

Staining of Rh30 rhabdomyosarcoma (upper panels) and SJSA-1 Ewing’s sarcoma  cells (lower panels) for GAPDH with ACR001P anti-GAPDH monoclonal antibody (left panels).  FITC conjugated secondary antibody. The middle panels show DAPI staining of the cell nuclei. The right panels show merged images. Mouse IgG1 6C5 Purified Can, Fe, Fi, Hu, Ms, Por, Rb, Rt E, ICC/IF, IP, WB
0.1 mg / €170.00
  Acris Antibodies GmbH

GAPDH (Loading Control) antibody

Staining of Rh30 rhabdomyosarcoma (upper panels) and SJSA-1 Ewing’s sarcoma  cells (lower panels) for GAPDH with ACR001P anti-GAPDH monoclonal antibody (left panels).  FITC conjugated secondary antibody. The middle panels show DAPI staining of the cell nuclei. The right panels show merged images. Mouse IgG1 6C5 Purified Can, Fe, Fi, Hu, Ms, Por, Rb, Rt E, ICC/IF, IP, WB
20 µg / €60.00
  Acris Antibodies GmbH

GAPDH (C-term) antibody

TA302944 staining (0.01µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAPDH antibody

Western blot of rat hippocampal lysate showing the immunolabeling of ~38k GAPDH protein. Mouse IgM 1D4 Supernatant Bov, Chk, Hu, Ms, Por, Rt C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAPDH antibody

GAPDH Rabbit IgG Azide Free Bakers Yeast Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €240.00
  Acris Antibodies GmbH

GAPDH antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Human GAPDH (SP2162P) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

GAPDH antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Human GAPDH (SP2162P) Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €300.00
  Acris Antibodies GmbH

GAPDH antibody

GAPDH antibody detects GAPDH protein by Western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug NIH-3T3 whole cell lysate/extract. C. 30 ug mouse brain lysate/extract. D. 30 ug PC-12 whole cell lysate/extract. E. 30 ug rat brain lysate/ex Rabbit IgG Aff - Purified Dros, Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAPDH (N-term) antibody

Western blot analysis of GAPDH Antibody (N-term) (Cat.#TA325000) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GAPDH (C-term) antibody

Western blot analysis of GAPDH Antibody (C-term R248) (Cat.#TA325001) in A2058, A375, CEM cell line lysates (35ug/lane). GAPDH (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GAPDH antibody

Western Blot: GAPDH Antibody (1D4) [TA336621] - Zebrafish embryo lysates at different developmental stages probed with GAPDH monoclonal antibody 1D4. Data from confirmed customer review. Mouse IgM 1D4 Supernatant Bov, Chk, Hst, Hu, Ms, Rt ICC/IF, WB
0.25 ml / €325.00
  OriGene Technologies, Inc.

GAPDH antibody

Western Blot: GAPDH Antibody (NB615) [TA336628] - Analysis of 40ug HeLa whole cell lysates using TA336628 at a dilution of 1:100. Mouse IgM NB615 Purified Bov, Can, Chk, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAPDH antibody

Western Blot: GAPDH Antibody [TA336630] - Detection of Human and Mouse GAPDH by Western Blot. Samples: Whole cell lysate from mouse NIH3T3 (5, 15 and 50 ug) and human HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0. Rabbit Purified Hu, Ms, Prim, Rt C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAPDH antibody

Western Blot: GAPDH Antibody [TA336632] - Detection of Human and Mouse GAPDH Whole cell lysate from HeLa (5, 15 and 50 mcg) and mouse NIH3T3 (M; 50 mcg). Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/ml. Chemiluminescence with an exposure Rabbit Purified Hu, Ms, Xen C, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAPDH antibody

Western Blot: GAPDH Antibody [TA336635] - Detection of GAPDH in mouse liver. Rabbit Purified Bov, Chk, Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAPDH antibody

Western Blot: GAPDH Antibody (2D4A7) [TA336636] - Analysis using anti-GAPDH monoclonal antibody, against cell lysate (2: Hela, 3: K562, 4: Jurkat, 5: NIH/3T3, 6: HepG-2). Mouse IgG1 2D4A7 Purified Hu, Ms, Por, Rb, Rt C, E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GAPDH antibody

Western Blot: GAPDH Antibody (13H12) [TA337137] - analysis of GAPDH in HeLa and HEK 293 cells (25ug/lane) using anti-GAPDH antibody. Image from verified customer review. Mouse 13H12 Purified Dros, Hu, Ms, Prim C, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GAPDH antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GAPDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802524) Mouse IgG1 OTI2F9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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