GAPDH2 antibody

Principal name

GAPDH2 antibody

Alternative names for GAPDH2 antibody

GAPDHS, GAPD2, GAPDS, HSD35, HSD-35, Glyceraldehyde-3-phosphate dehydrogenase testis-specific, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2

SwissProt ID

O14556 (Human), Q2KJE5 (Bovin), Q64467 (Mouse)

Gene ID

26330 (GAPDHS)

Available reactivities

Hu (Human), Por (Porcine), Ms (Mouse), Can (Canine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Frozen Sections (C)

Background of GAPDH2 antibody

GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

General readings

1. Tepla O. et al. In: In vitro Fertilization & Human Reproductive Genetics, ed. Monduzzi, Bologna, 107, (1999).
2. Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J.: Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization. Ceska Gynekol. 2000 Jan;65(1):28-32. Czech.
3. Peknicova J, Kyselova V, Buckiova D, Boubelik M.: Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Am J Reprod Immunol. 2002 May;47(5):311-8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GAPDH2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GAPDHS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505454) Mouse IgG2a OTI1F6 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

GAPDH2 antibody

WB Suggested Anti-GAPDHS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Spleen Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GAPDH2 antibody

GAPDH2 Mouse IgM Hs-8 Purified Hu, Por F, ICC/IF, WB
0.1 mg / €240.00
  Acris Antibodies GmbH

GAPDH2 antibody

Immunoprecipitation of GAPDHS transfected lysate using anti-GAPDHS MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GAPDHS MaxPab mouse polyclonal antibody (B01) (H00026330-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

GAPDH2 antibody

Western Blot analysis of GAPDHS expression in transfected 293T cell line (H00026330-T02) by GAPDHS MaxPab polyclonal antibody.

Lane 1: GAPDHS transfected lysate(44.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

GAPDH2 antibody

Immunoperoxidase of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml] Mouse IgG1 2E3-2E10 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GAPDH2 antibody

Human Spleen; WB Suggested Anti-GAPDHS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; GAPDHS antibody - N-terminal region (ARP40187_P050) in Human Spleen cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

GAPDH2 antibody

Western Blot: GAPDHS Antibody [NBP1-55234] - This Anti-GAPDHS antibody was used in Western Blot of Fetal Spleen   tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GAPDH2

GAPDH2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

GAPDH2

GAPDH2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GAPDH2

GAPDH2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

GAPDHS (Human) Matched Antibody Pair

GAPDHS (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GAPDHS 293T Cell Transient Overexpression Lysate(Denatured)

GAPDHS 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

GAPDHS overexpression lysate

GAPDHS overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

14 Item(s)

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