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Gastrin-releasing peptide receptor antibody

Principal name

Gastrin-releasing peptide receptor antibody

Alternative names for Gastrin-releasing peptide receptor antibody

GRP-R, GRPR, GRP-preferring bombesin receptor

SwissProt ID

P21729 (Mouse), P30550 (Human), P52500 (Rat)

Gene ID

2925 (GRPR), 14829 (Grpr), 24938

Ncbi ID

9606, NP_005305, NP_005305.1, NP_032203.1, NM_008177, NM_012706, NM_005314

Available reactivities

Hu (Human), Bt (Bat), Bov (Bovine), Can (Canine), Eq (Equine), Hst (Hamster), Marmoset, Mky (Monkey), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Gorilla, Chk (Chicken)

Available hosts

Rabbit, Mouse

Available applications

Flow Cytometry (F), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Gastrin-releasing peptide receptor antibody

Bombesin receptor 2 (BB2) is a member of a family of receptors that binds the 14 amino acid peptide, bombesin. Bombesin was first isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP; a 27 amino acid homologue) and neuromedin B (NMB; a 10 amino acid homologue). BB2 is the preferred receptor for GRP and hence it is also referred to as GRP receptor or GRP-R. BB1 is the preferred receptor for NMB and therefore it is also known as NMB-R. A third member (called BB3) of the bombesin receptor family has been recognized but the endogenous mammalian ligand for this receptor remains to be identified. All three bombesin receptors are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB2 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth. GRP (through its binding to BB2) plays an essential role in the release of hormones in the intestil tract, the regulation of smooth muscle contraction, and the secretion of pancreatic enzymes. In the brain, BB2 has been implicated in regulating feeding behavior, thermoregulation, and memory formation. Filly, BB2 is overexpressed in several human tumors including breast, prostate, and lung, where it acts as a growth factor receptor inducing tumor growth. BB2 is considered a potential target for the development of both diagnostics and anti-cancer therapies. Alomone labs is pleased to offer a highly specific antibody directed against an epitope located in the 3rd extracellular loop of the human BB2 receptor. Anti-Bombesin Receptor 2 (extracellular) antibody (#ABR-002) can be used in western blot alysis, as well as immunocytochemical and immunohistochemical applications, and will recognize BB2 from human, rat, mouse, and dog samples.

General readings

Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009)
Chao, C., et al. J. Surg. Res. 156(1):26-31(2009)
Ananias, H.J., et al. Prostate 69(10):1101-1108(2009)
Fleischmann, A., et al. Endocr. Relat. Cancer 16(2):623-633(2009)

AP51959PU-N - Gastrin-releasing peptide receptor

Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue reacted with GRPR Antibody (Center) Cat.-No AP51959PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.

AP51959PU-N

Western blot analysis of GRPR Antibody (Center) Cat.-No AP51959PU-N in HL-60 cell line lysates (35ug/lane). GRPR (arrow) was detected using the purified Pab.

AP51959PU-N

Flow cytometric analysis of HL-60 cells using GRPR Antibody (Center) Cat.-No AP51959PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AP51959PU-N

TA316872 - Gastrin-releasing peptide receptor

Anti-GRPR antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA316872

Anti-GRPR antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA316872

Anti-GRPR antibody IHC of human pancreas, islet of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA316872

TA316873 - Gastrin-releasing peptide receptor

Anti-GRPR antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

TA316873

TA321704 - Gastrin-releasing peptide receptor

Western blot analysis of Human hepatocellular carcinoma tissue and A549 cell using TA321704(GRPR Antibody) at dilution 1/300

TA321704

Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA321704(GRPR Antibody)(left image) at dilution 1/40, the image on the right is treated with the synthetic peptide.(Original magnification: x200)

TA321704

Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA321704 (GRPR Antibody)(left image) at dilution 1/40, the image on the right is treated with the synthetic peptide.(Original magnification: x200)

TA321704

TA321705 - Gastrin-releasing peptide receptor

Predicted band size: 43 kDa . Positive control: Mouse pancreas tissue and A549 cell lysate. Recommended dilution: 1/1000-2000. (Gel: 10%SDS-PAGE Lane1: Mouse pancreas tissue lysate Lane2: A549 cell lysate Lysates: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA321705

Predicted cell location: Cell membrane. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using GRPR antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)

TA321705

TA328635 - Gastrin-releasing peptide receptor

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: 1, 2. Anti-Bombesin Receptor 2 (extracellular) antibody, (1:500). 3, 4. Anti-Bombesin Receptor 2 (extracellular) antibody, preincubated with the control peptide antigen.

TA328635

Western blot analysis of human DU-145 (lanes 1 and 3) and PC-3 (lanes 2 and 4) prostate carcinoma cell lines: 1, 2. Anti-Bombesin Receptor 2 (extracellular) antibody, (1:200). 3, 4. Anti-Bombesin Receptor 2 (extracellular) antibody, preincubated with the control peptide antigen.

TA328635

Expression of Bombesin Receptor 2 in human colon. Immunohistochemical staining of paraffin-embedded human colon using Anti-Bombesin Receptor 2 (extracellular) antibody, (1:50). (A and B) Human colon showing malignant growth. Staining is specific for epithelium-derived malignant cells. (C and D) Normal colon, staining is specific for absorptive epithelial cells in the crypts of Lieberkuhn. Histofine (pink) is used for the color reaction. Hematoxilin is used as the counterstain.

TA328635

Expression of Bombesin Receptor 2 in human HT-29 cells. Immunocytochemical staining of live intact human HT-29 (colorectal adenocarcinoma) cells. Cells were stained with Anti-Bombesin Receptor 2 (extracellular) antibody(1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody, showing surface expression of the BB2 receptor.

TA328635

CPA9248-100ul - Gastrin-releasing peptide receptor

Western blot analysis of GRPR expression in mouse brain (A); rat brain (B) whole cell lysates.

Immunohistochemical analysis of GRPR staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA328635	Gastrin-releasing peptide receptor antibody
	Rabbit Polyclonal Anti-Bombesin Receptor 2 (extracellular)
	Rabbit		Purified	Hu, Ms, Rt	WB	0.2 ml / €622.00
						OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
TA316872	Gastrin-releasing peptide receptor (Extracell. Dom.) antibody
	GRPR Rabbit Polyclonal (Extracellular Domain) Antibody
	Rabbit		Aff - Purified	Bov, Bt, Can, Eq, Gorilla, Hst, Hu, Marmoset, Mky, Ms, Por, Rb, Rt	P, WB	50 µg / €400.00
						OriGene Technologies, Inc.
TA316873	Gastrin-releasing peptide receptor (Cytopl. Dom.) antibody
	GRPR Rabbit Polyclonal (Cytoplasmic Domain) Antibody
	Rabbit		Aff - Purified	Bov, Bt, Can, Chk, Eq, Hst, Hu, Marmoset, Mky, Ms, Por, Rb, Rt	ICC/IF, P	50 µg / €400.00
						OriGene Technologies, Inc.
TA321704	Gastrin-releasing peptide receptor antibody
	Anti-GRPR Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+2 additional images
TA321705	Gastrin-releasing peptide receptor antibody
	Anti-GRPR Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	E, P, WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
+1 additional image
AP51959PU-N	Gastrin-releasing peptide receptor (Center) antibody
	Rabbit	Ig	Aff - Purified	Hu	F, P, WB	0.4 ml / €370.00
						OriGene Technologies GmbH
+2 additional images

Human Lenti ORF Particles

Catalog No.
RC210362L2V	Lenti ORF particles, GRPR (mGFP-tagged) - Human gastrin-releasing peptide receptor (GRPR), 200 uL, >10^7 TU/mL
		200 µl / €1,082.00
		OriGene Technologies, Inc.

RC210362L1V	Lenti ORF particles, GRPR (Myc-DDK tagged) - Human gastrin-releasing peptide receptor (GRPR) , 200 uL, >10^7 TU/mL
		200 µl / €969.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR224721L2V	Lenti ORF particles, Grpr (GFP-tagged) - Mouse gastrin releasing peptide receptor (Grpr), 200 uL, >10^7 TU/mL
		200 µl / €907.00
		OriGene Technologies, Inc.

MR224721L1V	Lenti ORF particles, Grpr (Myc-DDK-tagged) - Mouse gastrin releasing peptide receptor (Grpr), 200 uL, >10^7 TU/mL
		200 µl / €804.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR201544L2V	Lenti ORF particles, Grpr (GFP-tagged ORF) - Rat gastrin releasing peptide receptor (Grpr), 200 uL, >10^7 TU/mL
		200 µl / €907.00
		OriGene Technologies, Inc.

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