GATA1 antibody

Principal name

GATA1 antibody

Alternative names for GATA1 antibody

GATA-1, ERYF1, GF1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, GATA-1, GF-1, NF-E1 DNA-binding protein

SwissProt ID

P15976 (Human)

Gene ID

2623 (GATA1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine)

Available hosts

Rat, Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of GATA1 antibody

Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4, expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.

General readings

1. Fu W  et al. A multiply redundant genetic switch 'locks in' the transcriptional signature of regulatory T cells. Nat Immunol 13:972-80 (2012).
2. Boidot R  et al. The transcription factor GATA-1 is overexpressed in breast carcinomas and contributes to survivin upregulation via a promoter polymorphism. Oncogene 29:2577-84 (2010). WB, IHC-P ; Human .
3. Hoeller S  et al. New GATA1 mutation in codon 2 leads to the earliest known premature stop codon in transient myeloproliferative disorder. Blood 114:3717-8 (2009). IHC-P ; Human .
4. Chen S  et al. Transcriptional Co-activator p300 Regulates Glucose Induced Gene Expression in the Endothelial Cells. Am J Physiol Endocrinol Metab : (2009). WB ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GATA1 antibody

TA303049 (0.3µg/ml) staining of human PBMC lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GATA1 antibody

Predicted band size: 43 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GATA1 antibody

Western blot analysis of GATA1 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GATA1 antibody

Western blot analysis of GATA1 expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GATA1 antibody

WB Suggested Anti-GATA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

GATA1 antibody

Western blot analysis using GATA1 mouse mAb against K562 (1) cell lysate. Mouse IgG1 4G1 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

GATA1 antibody

Immunohistochemical analysis of paraffin-embedded pancreatic cancer, using GATA1 mouse mAb with DAB staining. Mouse IgG2b 4F5 Ascites E, ICC/IF, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

GATA1 pSer310 antibody

GATA1 Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GATA1 pSer142 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA-1 (pSer142) antibody (left) or the same antibody preincubated with blocking peptide (right) Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

GATA1 pSer142 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA-1 (pSer142) antibody (left) or the same antibody preincubated with blocking peptide (right) Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

GATA1 pSer310 antibody

Immunofluorescence staining of methanol-fixed Hela cells using GATA1 (pSer310) Antibody Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

GATA1 pSer310 antibody

Immunofluorescence staining of methanol-fixed Hela cells using GATA1 (pSer310) Antibody Rabbit Aff - Purified Hu, Ms ICC/IF, WB
50 µl / €240.00
  Acris Antibodies GmbH

GATA1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA-1 antibody (Cat#AP02583PU) (Left) or the same antibody preincubated with blocking peptide (Right). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

GATA1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA-1 antibody (Cat#AP02583PU) (Left) or the same antibody preincubated with blocking peptide (Right). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

GATA1 antibody

Western blot analysis of extracts from COS7 cells using GATA1 antibody (Line1 and 2) and GATA1 (pSer310) antibody. (Line 3 and 4) Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €270.00
  Acris Antibodies GmbH

GATA1 antibody

Western blot analysis of extracts from COS7 cells using GATA1 antibody (Line1 and 2) and GATA1 (pSer310) antibody. (Line 3 and 4) Rabbit Aff - Purified Hu, Ms WB
50 µl / €230.00
  Acris Antibodies GmbH

GATA1 antibody

Western blot (WB) analysis of GATA1 antibody (Cat.-No.: AP06135PU-N) in extracts from Hela cell. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GATA1 pSer142 antibody

Immunohistochemistry (IHC) analyzes of p-GATA-1 (pSer142) antibody (Cat.-No.: AP20945PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

GATA1 pSer310 antibody

Immunohistochemistry (IHC) analyzes of p-GATA-1 antibody (Cat.-No.: AP20946PU-N) in paraffin-embedded human lung adenocarcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

GATA1 antibody

Immunohistochemistry (IHC) analyzes of GATA-1 antibody (Cat.-No.: AP21034PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

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