GATA3 antibody

Principal name

GATA3 antibody

Alternative names for GATA3 antibody

GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3

SwissProt ID

P23771 (Human), P23772 (Mouse), P23773 (Xenla), P23825 (Chick), Q08DV0 (Bovin)

Gene ID

2625 (GATA3), 14462 (Gata3)

Available reactivities

Hu (Human), Ms (Mouse), Bt (Bat), Bov (Bovine), Can (Canine), Chk (Chicken), Fi (Fish), Hst (Hamster), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Rb (Rabbit), Rt (Rat), Sh (Sheep), Trout, Bear, Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Goat, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of GATA3 antibody

GATA3: GATA binding protein 3. The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells, macrophages, or HeLa cell lines. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1,but no other structural homologies between the 2 genes could be found.

General readings

Cook, K.D., et al. J. Immunol. 185(6):3209-3216(2010)
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Kemp, K.L., et al. J. Immunol. 184(8):4178-4184(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Yao, X., et al. Chin. Med. J. 123(6):739-744(2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GATA3 antibody

WB Suggested Anti-GATA3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysateThere is BioGPS gene expression data showing that GATA3 is expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

GATA3 antibody

WB Suggested Antibody Titration: 0.2-1 ug/ml; Positive Control: JurkatThere is BioGPS gene expression data showing that GATA3 is expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GATA3 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809094)(1:200) Mouse IgG2b OTI5C11 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GATA3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809095)(1:150)(1:200) Mouse IgG1 OTI8H4 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GATA3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GATA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809219)(1:150) Mouse IgG2b OTI10G7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GATA3 antibody

Human kidney; Immunohistochemistry with Human kidney lysate tissue at an antibody concentration of 5.0ug/ml using anti-GATA3 antibody (ARP31857_P050); GATA3 antibody - N-terminal region (ARP31857_P050) in Human kidney cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

GATA3 antibody

Human Liver; Rabbit Anti-GATA3 Antibody Catalog Number: ARP32548 Paraffin Embedded Tissue: Human Liver Cellular Data: Hepatocyte Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X; GATA3 antibody - C-terminal region (ARP32548_P050) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

GATA3 antibody

Human Liver; Rabbit Anti-GATA3 Antibody. Catalog Number: ARP32548. Paraffin Embedded Tissue: Human Liver. Cellular Data: Hepatocyte. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; GATA3 antibody - C-terminal region (ARP32548_T100) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

GATA3 (N-term) antibody

TA305795 (1.0µg/ml) staining of Molt4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GATA3 antibody

Western blot analysis of GATA3 in human brain tissue lysate with GATA3 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GATA3 antibody

Immunohistochemistry analysis of paraffin-embedded human placenta tissue using GATA3 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GATA3 antibody

Western blot using 2 ug/mL Anti-Human/Mouse Gata-3 Purified) and revealed with Anti-Rat IgG HRP. Lane 1: 3 day anti-CD3/CD28 stimulated human CD4 cells, 2: 6 day Th2 polarized cultured human cells, 3: Jurkat cell line, 4: MCF7 cell line Rat TWAJ Purified Hu, Ms ICC/IF, WB
25 µg / €120.00
  OriGene Technologies, Inc.

GATA3 antibody

Western blot analysis of GATA3 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GATA3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:2000) Mouse IgG2b OTI1A5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GATA3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:2000) Mouse IgG1 OTI5F1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GATA3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:500) Mouse IgG2b OTI11H3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GATA3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:2000) Mouse IgG2b OTI8E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GATA3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GATA3 (RC211904, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GATA3.(1:2000) Mouse IgG2b OTI4D5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GATA3 antibody

Western blot analysis using GATA3 antibody Cat.-No AM06216SU-N against truncated GATA3-His recombinant protein (Lane 1). Mouse IgG1 1A10D1 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

GATA3 antibody

Immunohistochemical analysis of paraffin-embedded human Small Intestine, muscularis propria tissues using anti-GATA3 mouse mAb Mouse IgG1 7B5 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

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