GATA4 antibody

Principal name

GATA4 antibody

Alternative names for GATA4 antibody

GATA-4, GATA-binding factor 4, Transcription factor GATA-4

SwissProt ID

P43691 (Chick), P43694 (Human), P46152 (Rat), Q08369 (Mouse), Q0Q0E4 (Canfa), Q91677 (Xenla)

Gene ID

2626 (GATA4)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Mky (Monkey), Chk (Chicken), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Rat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of GATA4 antibody

Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.

General readings

1. Elabd C  et al. DNA methyltransferase-3-dependent nonrandom template segregation in differentiating embryonic stem cells. J Cell Biol 203:73-85 (2013).
2. He Z  et al. Transduction of Wnt11 promotes mesenchymal stem cell transdifferentiation into cardiac phenotypes. Stem Cells Dev 20:1771-8 (2011). WB ; Rat .
3. Mamidi MK  et al. Application of multiplex PCR for characterization of human embryonic stem cells (hESCs) and its differentiated progenies. J Biomol Screen 15:630-43 (2010).
4. Wilson KD  et al. Dynamic microRNA expression programs during cardiac differentiation of human embryonic stem cells: role for miR-499. Circ Cardiovasc Genet 3:426-35 (2010). WB; Human .
5. Li H  et al. Paracrine factors released by GATA-4 overexpressed mesenchymal stem cells increase angiogenesis and cell survival. Am J Physiol Heart Circ Physiol 299:H1772-81 (2010). ICC/IF ; Rat .
6. Chen S  et al. Transcriptional Co-activator p300 Regulates Glucose Induced Gene Expression in the Endothelial Cells. Am J Physiol Endocrinol Metab : (2009). WB ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GATA4 antibody

Anti-GATA4 mouse monoclonal antibody (TA500253) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GATA4(RC210945). Mouse IgG2b OTI1H8 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GATA4 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GATA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500091) Mouse IgG3 OTI9F9 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

GATA4 (C-term) antibody

Western blot analysis of anti-GATA4 Antibody (C-term) (Cat.#TA302164) in CEM cell line lysates (35ug/lane). GATA4(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GATA4 antibody

Anti-Human/Mouse Gata-4 Purified (2 ug/ml) was used in a western blot of SDS lysates. Lane 1: NIH-3T3, Lane 2: C57Bl/6 mouse heart, Lane 3: BALB/c mouse heart. Rat eBioEvan Purified Hu, Ms P, WB
25 µg / €120.00
  OriGene Technologies, Inc.

GATA4 antibody

Predicted cell location: Nucleus. Positive control: Human brain tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using GATA4 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GATA4 antibody

Western blot analysis of GATA4 expression in Serum treated COLO205 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GATA4 antibody

Western blot analysis of GATA4 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GATA4 antibody

Western blot analysis of extracts from Jurkat cells, using GATA4 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GATA4 antibody

Western Blot: GATA4 Antibody (6H10) [TA336777] - Western blot analysis using anti-GATA4 mAb against rat fetal heart (1) and adult heart (2) tissues lysate. Mouse IgG2b 6H10 Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GATA4 antibody

Western blot analysis using GATA4 antibody Cat.-No AM06352SU-N against rat fetal heart (Lane 1) and adult heart (Lane 2) tissues lysate. Mouse IgG2b 6H10 Ascites Hu, Ms E, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

GATA4 antibody

Immunohistochemistry (IHC) analyzes of GATA-4 antibody (Cat.-No.: AP06693PU-N) in paraffin-embedded human heart tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GATA4 antibody

Western blot analysis of GATA4 antibody (AP20231PU-N) in extracts from COLO205 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GATA4 antibody

Immunohistochemistry analyzes of GATA4 antibody (Cat.-No.: AP20302PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GATA4 antibody

GATA4 Mouse IgG2a 6C6 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GATA4 antibody

Western blot analysis of GATA4 ( arrow ) using rabbit GATA4 polyclonal antibody ( Cat # PAB2480 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the GATA4 gene ( Lane 2 ) ( Origene Technologies ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

GATA4 pSer262 antibody

Immunofluorescence analysis of HuvEc cells, using GATA4 (Phospho-Ser262) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

GATA4 pSer262 antibody

Immunofluorescence analysis of HuvEc cells, using GATA4 (Phospho-Ser262) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

GATA4 pSer105 antibody

Immunofluorescence analysis of HepG2 cells, using GATA4 (Phospho-Ser105) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

GATA4 pSer105 antibody

Immunofluorescence analysis of HepG2 cells, using GATA4 (Phospho-Ser105) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

GATA4 antibody

Immunofluorescence analysis of HuvEc cells, using GATA4 (Ab-262) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

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