GCLC / GLCLC antibody

Principal name

GCLC / GLCLC antibody

Alternative names for GCLC / GLCLC antibody

GCS heavy chain, GLCL, GLCLC, Gamma-ECS, Glutamate-cysteine ligase catalytic subunit, Gamma-glutamylcysteine synthetase

SwissProt ID

P19468 (Rat), P48506 (Human), P97494 (Mouse)

Gene ID

2729 (GCLC), 25283 (Gclc), 14629

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Ze (Zebrafish), Can (Canine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Eq (Equine), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of GCLC / GLCLC antibody

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene for catalytic subunit (GLCLC) encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kD and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p21-p22. GLCLC deficiency in human is associated with enzymopathic hemolytic anemia.
Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalyzed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cysteine Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlled by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH.

General readings

Man, B.L., et al. J Clin Neurosci 17(10):1244-1247(2010)
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010)
Le, T.M., et al. Mol. Genet. Metab. 101(1):55-61(2010)
Fullerton, J.M., et al. Bipolar Disord 12(5):550-556(2010)
Wang, X., et al. PLoS ONE 5 (8), E11934 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GCLC / GLCLC antibody

  GCLC mouse monoclonal antibody, clone OTI1A3 (formerly 1A3)  
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507318) Mouse IgG1 OTI1A3 Purified Hu, Mky, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GCLC / GLCLC (N-term) antibody

Confocal immunofluorescent analysis of GCLC / GLCLC Antibody (N-term) Cat.-No AP51800PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GCLC / GLCLC antibody

  Rabbit Polyclonal Anti-GCLC Antibody  
WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Can, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GCLC / GLCLC antibody

  Rabbit Polyclonal Anti-GCLC Antibody  
WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Placenta Rabbit IgG Purified Can, Eq, Hu, Por, Rb, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

GCLC / GLCLC (295-313) antibody

Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bov, Hu, Mky, Ms, Rt E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

GCLC / GLCLC antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

GCLC - human gene knockout kit via CRISPR

GCLC - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GCLC - human gene knockout kit via CRISPR

GCLC - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GCLC - human gene knockout kit via CRISPR

GCLC - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GCLC - human gene knockout kit via CRISPR

GCLC - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gclc - mouse gene knockout kit via CRISPR

Gclc - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Gclc - mouse gene knockout kit via CRISPR

Gclc - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gclc - mouse gene knockout kit via CRISPR

Gclc - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gclc - mouse gene knockout kit via CRISPR

Gclc - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GCLC / GLCLC

  Recombinant protein of human glutamate-cysteine ligase, catalytic subunit (GCLC)  
GCLC / GLCLC Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GCLC (mGFP-tagged)-Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, GCLC (mGFP-tagged)-Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,250.00
  OriGene Technologies, Inc.

Lenti ORF particles, GCLC (Myc-DDK-tagged)-Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, GCLC (Myc-DDK-tagged)-Human glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,120.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gclc (GFP-tagged) - Mouse glutamate-cysteine ligase, catalytic subunit (Gclc), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gclc (GFP-tagged) - Mouse glutamate-cysteine ligase, catalytic subunit (Gclc), 200 uL, >10^7 TU/mL  
200 µl / €1,300.00
  OriGene Technologies, Inc.

Lenti ORF particles, Gclc (Myc-DDK-tagged) - Mouse glutamate-cysteine ligase, catalytic subunit (Gclc), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gclc (Myc-DDK-tagged) - Mouse glutamate-cysteine ligase, catalytic subunit (Gclc), 200 uL, >10^7 TU/mL  
200 µl / €1,170.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gclc (GFP-tagged ORF) - Rat glutamate-cysteine ligase, catalytic subunit (Gclc), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gclc (GFP-tagged ORF) - Rat glutamate-cysteine ligase, catalytic subunit (Gclc), 200 uL, >10^7 TU/mL  
200 µl / €1,300.00
  OriGene Technologies, Inc.

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