GCLC / GLCLC antibody

Principal name

GCLC / GLCLC antibody

Alternative names for GCLC / GLCLC antibody

GCS heavy chain, GLCL, GLCLC, Gamma-ECS, Glutamate-cysteine ligase catalytic subunit, Gamma-glutamylcysteine synthetase

SwissProt ID

P19468 (Rat), P48506 (Human), P97494 (Mouse)

Gene ID

2729 (GCLC), 25283 (Gclc)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Ze (Zebrafish), Can (Canine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Eq (Equine), Ye (Yeast)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C)

Background of GCLC / GLCLC antibody

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene for catalytic subunit (GLCLC) encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kD and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p21-p22. GLCLC deficiency in human is associated with enzymopathic hemolytic anemia.
Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalyzed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cysteine Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlled by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH.

General readings

Man, B.L., et al. J Clin Neurosci 17(10):1244-1247(2010)
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010)
Le, T.M., et al. Mol. Genet. Metab. 101(1):55-61(2010)
Fullerton, J.M., et al. Bipolar Disord 12(5):550-556(2010)
Wang, X., et al. PLoS ONE 5 (8), E11934 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GCLC / GLCLC antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GCLC mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507318) Mouse IgG1 OTI1A3 Purified Hu, Mky, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GCLC / GLCLC (N-term) antibody

Confocal immunofluorescent analysis of GCLC / GLCLC Antibody (N-term) Cat.-No AP51800PU-N with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GCLC / GLCLC antibody

Immunofluorescence of monoclonal antibody to GCLC on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 3H1 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GCLC / GLCLC antibody

AP15575PU GCS antibody staining Human Testis. Rabbit Purified Hu, Ms, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

GCLC / GLCLC antibody

AP15575PU GCS antibody staining Human Testis. Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €520.00
  Acris Antibodies GmbH

GCLC / GLCLC antibody

AP15575PU GCS antibody staining Human Testis. Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

GCLC / GLCLC antibody

WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Can, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GCLC / GLCLC antibody

WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Placenta Rabbit IgG Purified Can, Eq, Hu, Por, Rb, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

GCLC / GLCLC (295-313) antibody

Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bov, Hu, Mky, Ms, Rt E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

GCLC / GLCLC antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Purified Hu, Ms, Rt P
50 µg / €470.00
  Acris Antibodies GmbH

GCLC / GLCLC antibody

GCLC / GLCLC Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

GCLC / GLCLC antibody

GCLC / GLCLC Rabbit Hu P
0.1 ml / €390.00
  Abnova Taiwan Corp.

GCLC / GLCLC antibody

GCLC / GLCLC Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

GCLC / GLCLC antibody

GCLC / GLCLC Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

GCLC / GLCLC antibody

Western blot analysis of GCLC expression in HeLa (A); A431 (B); Jurkat (C); HepG2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

GCLC / GLCLC antibody

Western blot analysis of GCLC expression in HeLa (A); A431 (B); Jurkat (C); HepG2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

GCLC / GLCLC antibody

Western blot analysis of GCLC expression in HeLa (A); A431 (B); Jurkat (C); HepG2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

GCLC / GLCLC antibody

GCLC / GLCLC Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €310.00
  Novus Biologicals Inc.

GCLC / GLCLC antibody

GCLC / GLCLC Rabbit IgG Aff - Purified Hu P, WB
0.5 ml / €420.00
  Novus Biologicals Inc.

GCLC / GLCLC antibody

GCLC / GLCLC Rabbit IgG Aff - Purified Hu, Ms, Rt C, P, WB
1 ml / €460.00
  Novus Biologicals Inc.

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