GCLM / GLCLR antibody

Principal name

GCLM / GLCLR antibody

Alternative names for GCLM / GLCLR antibody

Gamma-ECS regulatory subunit, GCS light chain, Glutamate-cysteine ligase regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate-cysteine ligase modifier subunit

SwissProt ID

O09172 (Mouse), P48507 (Human), P48508 (Rat), Q2T9Y6 (Bovin)

Gene ID

2730 (GCLM)

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Chk (Chicken), Ms (Mouse), Ze (Zebrafish), African clawed frog, Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP), Frozen Sections (C)

Background of GCLM / GLCLR antibody

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Dickinson D.A., et al. (2005) Free Radic Biol Med 37(8): 1152-1159Inoue Y., et al. (2003) Int J Oncol 23(5): 1333-1339Robertson N.G., et al. (1995) Genomics 23(1): 42–50

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GCLM / GLCLR (C-term) antibody

Western blot analysis of GCLM Antibody (C-term) (Cat. #TA325093) in 293 cell line and mouse kidney tissue lysates (35ug/lane). GCLM (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GCLM / GLCLR antibody

Western blot analysis of extracts of various cell lines, using GCLM antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

GCLM / GLCLR antibody

Lanes: 1: 40ug mouse heart lysate, 2: 40ug mouse heart lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1: 10000; Gene Name: GCLM; Submitted by: Anonymous; Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rb, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

GCLM / GLCLR antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLM (RC207224, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCLM. Rabbit IgG Purified Hu, Mky, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GCLM / GLCLR antibody

Western blot using GCLM / GLCLR antibody cat.no. AM50016PU: The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT2D12 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

GCLM / GLCLR antibody

Western blot using GCLM / GLCLR antibody cat.no. AM50016PU: The cell lysates of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCLM antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT2D12 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

GCLM / GLCLR antibody

GCLM MaxPab polyclonal antibody. Western Blot analysis of GCLM expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GCLM / GLCLR antibody

Immunoprecipitation of GCLM transfected lysate using anti-GCLM MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GCLM MaxPab mouse polyclonal antibody (B01) (H00002730-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

GCLM / GLCLR antibody

Western Blot analysis of GCLM expression in transfected 293T cell line (H00002730-T01) by GCLM MaxPab polyclonal antibody.

Lane 1: GCLM transfected lysate(30.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

GCLM / GLCLR antibody

GCLM monoclonal antibody (M01), clone 2B8 Western Blot analysis of GCLM expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 2B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GCLM / GLCLR antibody

GCLM monoclonal antibody (M02), clone 2C6. Western Blot analysis of GCLM expression in PC-12 ( Cat # L012V1 ). Mouse IgG2a 2C6 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GCLM / GLCLR antibody

GCLM monoclonal antibody (M03), clone 3A10. Western Blot analysis of GCLM expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 3A10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GCLM / GLCLR antibody

Human Liver; Liver; GCLM antibody - middle region (ARP54624_P050) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

GCLM / GLCLR antibody

ELISA: GCLM Antibody [31430002] - Affinity Purified Rabbit Hu E
50 µg / €440.00
  Novus Biologicals Inc.

GCLM / GLCLR antibody

GCLM / GLCLR Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

GCLM / GLCLR antibody

Western Blot: GCLM Antibody [NBP1-56423] - This Anti-GCLM antibody was used in Western Blot of MCF7 tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

GCLM / GLCLR antibody

GCLM / GLCLR Mouse Aff - Purified Hu WB
0.1 mg / €400.00
  Novus Biologicals Inc.

GCLM / GLCLR antibody

GCLM / GLCLR Mouse Aff - Purified Hu WB
0.1 mg / €400.00
  Novus Biologicals Inc.

GCLM / GLCLR antibody

Immunohistochemistry-Paraffin: GCLM Antibody (EPR6668) [NBP1-95980] human muscle tissue using anti-GCLM. Rabbit EPR6668 Purified Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GCLM / GLCLR

GCLM / GLCLR Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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