GDF15 antibody

Principal name

GDF15 antibody

Alternative names for GDF15 antibody

GDF-15, MIC1, PDF, PLAB, PTGFB, Growth/differentiation factor 15, Placental bone morphogenetic protein, Placental TGF-beta, Macrophage inhibitory cytokine 1, MIC-1, Prostate differentiation factor, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1

SwissProt ID

Q99988 (Human), Q9Z0J6 (Rat), Q9Z0J7 (Mouse)

Gene ID

9518 (GDF15), 23886 (Gdf15), 29455

Available reactivities

Hu (Human), Chimp (Chimpanzee), Macaque, Ms (Mouse), Rt (Rat), Mky (Monkey), Por (Porcine)

Available hosts

Goat, Rabbit, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P)

Background of GDF15 antibody

Growth and differentiation factor-15 (GDF-15), also known as macrophage inhibitory cytokine-1(MIC-1), placental transforming growth factor-., prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-.) superfamily . GDF-15 is initially synthesized as a 40 kDa inactive precursor protein. It is then proteolytically cleaved to release the active C-terminal fragment, which is assembled into a disulfide-linked homodimer of 28 kDa to become biologically active GDF-15 . In humans, GDF-15 is predominantly expressed in the placenta, with low levels in the kidney,pancreas, and prostate. However, its expression can be rapidly induced by cytokines such as interleukin-1 and TGF-. .

GDF-15 has diverse biological functions. Early studies have shown that low serum GDF-15 levels correlate with miscarriages, indicating that it might be able to suppress inflammation in early pregnancy . GDF-15 also plays an important role in tumorigenesis and metastasis. It has been observed that in many types of cancers, such as colorectal, breast, and prostate, theexpression of GDF-15 is dramatically increased . Additionally, in cancer patients, serum levels of GDF-15 are elevated, which are of value in disease diagnosis and stratification (10 -12). GDF-15 is strongly induced by the tumor suppressor gene p53 and other anti-tumorigenic agents, such as the non-steroidal anti-inflammatory drugs and peroxisome proliferators-activated receptor .. These findings suggest that GDF-15 may be a downstream target of those signaling pathways that regulate cell cycle arrest and apoptosis . Through the modulation of neuronal pathways important in the regulation of appetite and energy homeostasis, GDF-15 mediates cancer-induced anorexia and weight loss .

General readings

Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.

Lawton, L.N. et al. (1997) Gene 203:17.

Paralkar, V.M. et al. (1998) J. Biol. Chem. 273:13760.

Bauskin, A.R. et al. (2000) EMBO J. 19:2212.

Moore, A.G. et al. (2000) J. Clin. Endocrinol. Metab. 85:4781.

Tong, S. et al. (2004) Lancet 363:129.

Buckhaults, P. et al. (2001) Cancer Res. 61:6996.

Welsh, J.B. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3410.

Cheung, P.K. et al. (2004) Cancer Res. 64:5929.

Koopmann, J. et al. (2004) Clinical Cancer Res. 10:2386.

Koopmann, J. et al. (2006) Clinical Cancer Res. 12:442.

Brown, D.A. et al. (2006) Clinical Cancer Res. 12:89.

Tan, M. et al. (2000) Proc. Natl. Acad. Sci. USA 97:109.

Li, P.X. et al. (2000) J. Biol. Chem. 75:20127.

Agarwal, M.K. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16278.

Johnen, H. et al. (2007) Nat. Med. 13:1333.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GDF15 antibody

  Rabbit anti-GDF15 Polyclonal Antibody  
Western blot analysis of SW620 cell lysate using GDF15 antibody. Rabbit IgG Purified Hu, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 (C-term) antibody

  Goat Polyclonal Antibody against GDF15  
TA302498 (0.1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

  Rabbit polyclonal anti-GDF15 antibody  
Western blot analysis of extracts from HepG2 cells and 293 cells, using GDF15 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GDF15 (30-45) antibody

  GDF15 Rabbit Polyclonal (aa30-45) Antibody  
Anti-GDF15 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rabbit Purified Hu E, P
50 µg / €400.00
  OriGene Technologies, Inc.

GDF15 (C-term) antibody

  GDF15 Goat Polyclonal (C-Terminus) Antibody  
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Goat IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €400.00
  OriGene Technologies, Inc.

GDF15 antibody

  GDF15 Rat Monoclonal (6D12.H10.E4) Antibody  
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml. Rat IgG 6D12.H10.E4 Purified Hu, Ms
50 µg / €400.00
  OriGene Technologies, Inc.

GDF15 (D variant) antibody

  Rabbit polyclonal anti-Nag-1 antibody  
In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies : anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. 20 random normal human sera were performed. HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 µL per well at 1 µg/mL. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody

  Rabbit polyclonal Nag-1 antibody Peroxidase Conjugated  
Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 (H variant) antibody

  Rabbit polyclonal anti-Nag-1 antibody  
In this ELISA, NAG-1 was captured from human serum using the following antibodies): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody

  Rabbit polyclonal Nag-1 antibody Biotin Conjugated  
Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 (N-term) (H/D variant) antibody

  Rabbit polyclonal anti-Nag-1 antibody  
Western blot using affinity purified anti-NAG-1/GDF15 (N-terminal specific) antibody shows detection of a 14 kDa band corresponding to recombinant human NAG-1 purified from CHO cells.   Samples were electrophoresed on a 4-20% gradient gel under reducing conditions.   Lane 1 shows NAG-1 detection.  Lane 2 shows reactivity is greatly diminished when this antibody is preincubated with the immunizing peptide prior to Western blotting.  Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 (C-term) antibody

  Rabbit polyclonal anti-NAG-1 antibody  
WB using Anti-Mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody. Rabbit IgG Ig Fraction Hu, Ms E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody

  Rabbit polyclonal anti-Nag-1 antibody  
WB using Anti-mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody. Rabbit IgG Purified Hu, Ms E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody

  Mouse monoclonal NAG-1 H Variant antibody  
WB shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1,000. For detection, peroxidase conjugated Gt-a-Mouse IgG was used at 1:40000. Mouse 7C12.B3.F2 Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody

  Mouse monoclonal anti-NAG-1 antibody  
WB shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1,000. HRP conjugated Gt-a-Mouse IgG secondary antibody was used AT 1:40000. Mouse 23B3D2.H5 Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody

  Mouse monoclonal anti-NAG-1 antibody  
WB shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag; lane 3 - yeast cell lysate expressing NAG-1 H variant; lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1000. For detection, HRP conjugated Gt-a-Mouse IgG secondary antibody was used at 1:40000. Mouse 23G10.F8 Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody

  Anti-GDF15 Rabbit Polyclonal Antibody  
Predicted band size: 34 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

  Anti-GDF15 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human renal cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human renal cancer tissue using GDF15 antibody at dilution 1/7, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GDF15 (C-term) antibody

Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

GDF15 (C-term) antibody

Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

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