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GDF15 antibody

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Principal name

GDF15 antibody

Alternative names for GDF15 antibody

GDF-15, MIC1, PDF, PLAB, PTGFB, Growth/differentiation factor 15, Placental bone morphogenetic protein, Placental TGF-beta, Macrophage inhibitory cytokine 1, MIC-1, Prostate differentiation factor, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1

SwissProt ID

Q99988 (Human), Q9Z0J6 (Rat), Q9Z0J7 (Mouse)

Gene ID

9518 (GDF15), 23886 (Gdf15), 29455

Ncbi ID

NP_004855, 9606, NP_004855.2, NP_035949, 10090, NM_004864, NM_011819, NM_019216

Available reactivities

Hu (Human), Chimp (Chimpanzee), Macaque, Ms (Mouse), Rt (Rat), Mky (Monkey), Por (Porcine)

Available hosts

Goat, Rabbit, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P)

Background of GDF15 antibody

Growth and differentiation factor-15 (GDF-15), also known as macrophage inhibitory cytokine-1(MIC-1), placental transforming growth factor-., prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-.) superfamily . GDF-15 is initially synthesized as a 40 kDa inactive precursor protein. It is then proteolytically cleaved to release the active C-terminal fragment, which is assembled into a disulfide-linked homodimer of 28 kDa to become biologically active GDF-15 . In humans, GDF-15 is predominantly expressed in the placenta, with low levels in the kidney,pancreas, and prostate. However, its expression can be rapidly induced by cytokines such as interleukin-1 and TGF-. .

GDF-15 has diverse biological functions. Early studies have shown that low serum GDF-15 levels correlate with miscarriages, indicating that it might be able to suppress inflammation in early pregnancy . GDF-15 also plays an important role in tumorigenesis and metastasis. It has been observed that in many types of cancers, such as colorectal, breast, and prostate, theexpression of GDF-15 is dramatically increased . Additionally, in cancer patients, serum levels of GDF-15 are elevated, which are of value in disease diagnosis and stratification (10 -12). GDF-15 is strongly induced by the tumor suppressor gene p53 and other anti-tumorigenic agents, such as the non-steroidal anti-inflammatory drugs and peroxisome proliferators-activated receptor .. These findings suggest that GDF-15 may be a downstream target of those signaling pathways that regulate cell cycle arrest and apoptosis . Through the modulation of neuronal pathways important in the regulation of appetite and energy homeostasis, GDF-15 mediates cancer-induced anorexia and weight loss .

General readings

Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.

Lawton, L.N. et al. (1997) Gene 203:17.

Paralkar, V.M. et al. (1998) J. Biol. Chem. 273:13760.

Bauskin, A.R. et al. (2000) EMBO J. 19:2212.

Moore, A.G. et al. (2000) J. Clin. Endocrinol. Metab. 85:4781.

Tong, S. et al. (2004) Lancet 363:129.

Buckhaults, P. et al. (2001) Cancer Res. 61:6996.

Welsh, J.B. et al. (2003) Proc. Natl. Acad. Sci. USA 100:3410.

Cheung, P.K. et al. (2004) Cancer Res. 64:5929.

Koopmann, J. et al. (2004) Clinical Cancer Res. 10:2386.

Koopmann, J. et al. (2006) Clinical Cancer Res. 12:442.

Brown, D.A. et al. (2006) Clinical Cancer Res. 12:89.

Tan, M. et al. (2000) Proc. Natl. Acad. Sci. USA 97:109.

Li, P.X. et al. (2000) J. Biol. Chem. 75:20127.

Agarwal, M.K. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16278.

Johnen, H. et al. (2007) Nat. Med. 13:1333.

PAB6858 - GDF15
HEK293 overexpressing GDF15 and probed with GDF15 polyclonal antibody ( Cat # PAB6858 ) ( mock transfection in first lane ), tested by Origene.
PAB6858 - GDF15
GDF15 polyclonal antibody ( Cat # PAB6858 ) ( 0.1 µg/ml ) staining of human prostate lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
AR09293PU-L - GDF15
Recombinant human GDF-15 (195-308 aa), His-tagged: 15% SDS-PAGE (3 µg)
AR09293PU-L - GDF15
AP09407PU-N - GDF15
Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
AP09408PU-S - GDF15
In this ELISA, NAG-1 was captured from human serum using the following antibodies): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C.
AP09409PU-S - GDF15
In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies : anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. 20 random normal human sera were performed. HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 µL per well at 1 µg/mL.
AP09410PU-S - GDF15
Western blot using affinity purified anti-NAG-1/GDF15 (N-terminal specific) antibody shows detection of a 14 kDa band corresponding to recombinant human NAG-1 purified from CHO cells.   Samples were electrophoresed on a 4-20% gradient gel under reducing conditions.   Lane 1 shows NAG-1 detection.  Lane 2 shows reactivity is greatly diminished when this antibody is preincubated with the immunizing peptide prior to Western blotting. 
DM1224 - GDF15
Figure 2. Capillary gel electrophoresis of purified ME-1C4 monoclonal antibody. Lane 1: Molecular Weight marker, Lane 2: purified ME-1C4 antibody. Proteins were separated by CGE (Capillary Gel Electrophoresis, Agilent 2100 Bioanalyzer). Internal Control bands (240 kDa / 7 kDa / 4.5 kDa).
DM1224 - GDF15
Figure.1: FACS analysis of BOSC23 cells using ME-6D1 (Cat.#DM1224). BOSC23 cells were transiently transfected with anexpression vector encoding either MIC-1 (red curve) or anirrelevant protein (Control transfectant: black curve). Binding of ME-6D1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with MIC-1 trans-fected cells.
ARP42200_P050 - GDF15
Human PANC1; WB Suggested Anti-GDF15 Antibody Titration: 0.2-1 ug/ml. Positive Control: PANC1 cell lysate. GDF15 is strongly supported by BioGPS gene expression data to be expressed in Human PANC1 cells.; GDF15 antibody - N-terminal region (ARP42200_P050) in Human PANC1 cells using Western Blot
AP21185PU-N - GDF15
Western blot (WB) analysis of GDF-15 antibody (Cat.-No.: AP21185PU-N) in extracts from HepG2 cells.
C15982-1 - GDF15
Western blot analysis of extracts from 293/HepG2 cells, using GDF15 Antibody. The lane on the right is treated with the synthesized peptide.
AP51810PU-N - GDF15
Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue reacted with GDF15 Antibody (N-term) Cat.-No AP51810PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
AP51810PU-N - GDF15
Western blot analysis of GDF15 Antibody (N-term) Cat.-No AP51810PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the GDF15 antibody detected the GDF15 protein (arrow).
AP51810PU-N - GDF15
Flow cytometric analysis of NCI-H460 cells using GDF15 Antibody (N-term) Cat.-No AP51810PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AR51733PU-N - GDF15
CEK1175 - Human GDF15 ELISA Kit
Representative Standard Curve
CEK1470 - Mouse GDF15 ELISA Kit
Representative Standard Curve
CPA3369-100ul - GDF15
Western blot analysis of GDF15 expression in HepG2 (A); NIH3T3 (B) whole cell lysates.
TP723131 - GDF15
TA302498 - GDF15
TA302498 (0.1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA312174 - GDF15
Western blot analysis of extracts from HepG2 cells and 293 cells, using GDF15 antibody.The lane on the right is treated with the synthesized peptide.
TA317816 - GDF15
Anti-GDF15 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
TA317816 - GDF15
Anti-GDF15 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
TA318039 - GDF15
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TA318039 - GDF15
Anti-GDF15 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TA318812 - GDF15
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
TA319179 - GDF15
Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
TA319179 - GDF15
Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in colon tissue at pH 6 . Negative control of human colon tissue pH6 is shown in background. Tissue: Human Colon
TA319179 - GDF15
Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in colon tissue at pH 9 . Negative control of human colon tissue pH9 is shown in background. Tissue: Human Colon
TA319181 - GDF15
Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting.
TA319181 - GDF15
Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in colon tissue at pH 6 . Negative control of human colon tissue pH6 is shown in background. Tissue: Human Colon
TA319181 - GDF15
Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific) at 20X in colon tissue at pH 9 . Negative control of human colon tissue pH9 is shown in background. Tissue: Human Colon
TA319470 - GDF15
WB using Anti-Mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody.
TA319471 - GDF15
WB using Anti-mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody.
TA319546 - GDF15
WB shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1,000. For detection, peroxidase conjugated Gt-a-Mouse IgG was used at 1:40000.
TA319547 - GDF15
WB shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1,000. HRP conjugated Gt-a-Mouse IgG secondary antibody was used AT 1:40000.
TA319548 - GDF15
WB shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag; lane 3 - yeast cell lysate expressing NAG-1 H variant; lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1000. For detection, HRP conjugated Gt-a-Mouse IgG secondary antibody was used at 1:40000.
TA321673 - GDF15
Predicted band size: 34 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA321674 - GDF15
Predicted cell location: Cytoplasm. Positive control: Human renal cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human renal cancer tissue using GDF15 antibody at dilution 1/7, on the right is treated with the fusion protein. (Original magnification: x200)
TA326764 - GDF15
Western blot analysis of SW620 cell lysate using GDF15 antibody.
TA326764 - GDF15
Immunohistochemistry of paraffin-embedded human lung cancer using GDF15 antibody at dilution of 1:200 (400x lens).
TA326764 - GDF15
Immunohistochemistry of paraffin-embedded rat kidney using GDF15 antibody at dilution of 1:200 (400x lens).
TA326764 - GDF15
Immunohistochemistry of paraffin-embedded rat lung using GDF15 antibody at dilution of 1:200 (400x lens).
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GDF15 antibody
  Rabbit anti-GDF15 Polyclonal Antibody  
Western blot analysis of SW620 cell lysate using GDF15 antibody. Rabbit IgG Purified Hu, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.
+3 additional images

Highly validated antibody!

GDF15 (C-term) antibody
  Goat Polyclonal Antibody against GDF15  
TA302498 (0.1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 antibody
  Rabbit polyclonal anti-GDF15 antibody  
Western blot analysis of extracts from HepG2 cells and 293 cells, using GDF15 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GDF15 (30-45) antibody
  GDF15 Rabbit Polyclonal (aa30-45) Antibody  
Anti-GDF15 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rabbit Purified Hu E, P
50 µg / €400.00
  OriGene Technologies, Inc.
+1 additional image

GDF15 (C-term) antibody
  GDF15 Goat Polyclonal (C-Terminus) Antibody  
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Goat IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €400.00
  OriGene Technologies, Inc.
+1 additional image

GDF15 antibody
  GDF15 Rat Monoclonal (6D12.H10.E4) Antibody  
Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml. Rat IgG 6D12.H10.E4 Purified Hu, Ms
50 µg / €400.00
  OriGene Technologies, Inc.

GDF15 (D variant) antibody
  Rabbit polyclonal anti-Nag-1 antibody  
In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies : anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. 20 random normal human sera were performed. HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 µL per well at 1 µg/mL. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody
  Rabbit polyclonal Nag-1 antibody Peroxidase Conjugated  
Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.
+2 additional images

GDF15 (H variant) antibody
  Rabbit polyclonal anti-Nag-1 antibody  
In this ELISA, NAG-1 was captured from human serum using the following antibodies): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody
  Rabbit polyclonal Nag-1 antibody Biotin Conjugated  
Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.
+2 additional images

GDF15 (N-term) (H/D variant) antibody
  Rabbit polyclonal anti-Nag-1 antibody  
Western blot using affinity purified anti-NAG-1/GDF15 (N-terminal specific) antibody shows detection of a 14 kDa band corresponding to recombinant human NAG-1 purified from CHO cells.   Samples were electrophoresed on a 4-20% gradient gel under reducing conditions.   Lane 1 shows NAG-1 detection.  Lane 2 shows reactivity is greatly diminished when this antibody is preincubated with the immunizing peptide prior to Western blotting.  Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 (C-term) antibody
  Rabbit polyclonal anti-NAG-1 antibody  
WB using Anti-Mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody. Rabbit IgG Ig Fraction Hu, Ms E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody
  Rabbit polyclonal anti-Nag-1 antibody  
WB using Anti-mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody. Rabbit IgG Purified Hu, Ms E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody
  Mouse monoclonal NAG-1 H Variant antibody  
WB shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1,000. For detection, peroxidase conjugated Gt-a-Mouse IgG was used at 1:40000. Mouse 7C12.B3.F2 Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody
  Mouse monoclonal anti-NAG-1 antibody  
WB shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1,000. HRP conjugated Gt-a-Mouse IgG secondary antibody was used AT 1:40000. Mouse 23B3D2.H5 Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody
  Mouse monoclonal anti-NAG-1 antibody  
WB shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag; lane 3 - yeast cell lysate expressing NAG-1 H variant; lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1000. For detection, HRP conjugated Gt-a-Mouse IgG secondary antibody was used at 1:40000. Mouse 23G10.F8 Purified Hu E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GDF15 antibody
  Anti-GDF15 Rabbit Polyclonal Antibody  
Predicted band size: 34 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GDF15 antibody
  Anti-GDF15 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human renal cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human renal cancer tissue using GDF15 antibody at dilution 1/7, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GDF15 (C-term) antibody
Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

GDF15 (C-term) antibody
Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

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