GDF15 antibody

Principal name

GDF15 antibody

Alternative names for GDF15 antibody

GDF-15, MIC1, PDF, PLAB, PTGFB, Growth/differentiation factor 15, Placental bone morphogenetic protein, Placental TGF-beta, Macrophage inhibitory cytokine 1, MIC-1, Prostate differentiation factor, NSAID-activated gene 1 protein, NAG-1, NSAID-regulated gene 1 protein, NRG-1

SwissProt ID

Q99988 (Human), Q9Z0J6 (Rat), Q9Z0J7 (Mouse)

Gene ID

9518 (GDF15), 23886

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chimp (Chimpanzee), Macaque, Mky (Monkey), Por (Porcine), Gorilla

Available hosts

Goat, Rabbit, Mouse, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Radioimmunoassay (R)

Background of GDF15 antibody

Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

General readings

Wollmann W, Goodman ML, Bhat-Nakshatri P, Kishimoto H, Goulet RJ Jr, Meharotra S, Morimiya A, Badve S, Nakshatri H. The macrophage inhibitory cytokine integrates AKT/PKB and MAP kinase signaling pathways in breast cancer cells.Carcinogenesis. 2005 Jan 27; [Epub ahead of print] PMID: 15677629

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GDF15 antibody

Western blot analysis of SW620 cell lysate using GDF15 antibody. Rabbit IgG Purified Hu, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 (C-term) antibody

TA302498 (0.1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

Western blot analysis of extracts from HepG2 cells and 293 cells, using GDF15 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GDF15 (30-45) antibody

Anti-GDF15 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rabbit Purified Hu E, P
50 µg / €345.00
  OriGene Technologies, Inc.

GDF15 (C-term) antibody

Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Goat IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

GDF15 antibody

Anti-GDF15 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml. Rat IgG 6D12.H10.E4 Purified Hu, Ms
50 µg / €345.00
  OriGene Technologies, Inc.

GDF15 (D variant) antibody

In this sandwich ELISA, NAG-1 was captured from human serum using the following antibodies : anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. 20 random normal human sera were performed. HRP conjugated anti-NAG-1/GDF15 (C terminal specific) antibody was added for detection at 100 µL per well at 1 µg/mL. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 (H variant) antibody

In this ELISA, NAG-1 was captured from human serum using the following antibodies): anti-NAG-1/GDF15 (C terminal specific), anti-NAG-1/GDF15 (N terminal specific (PAN)), anti-NAG-1/GDF15 (H-variant) and anti-NAG-1/GDF15 (D-variant) polyclonal antibodies. Micro titer plates were coated with capture antibody at 1 µg/mL. After overnight incubation and blocking, independent experiments using 20 random normal human sera were performed. Neat normal sera were applied and incubated for 1 h at 37 °C. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electro-phoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 (N-term) (H/D variant) antibody

Western blot using affinity purified anti-NAG-1/GDF15 (N-terminal specific) antibody shows detection of a 14 kDa band corresponding to recombinant human NAG-1 purified from CHO cells.   Samples were electrophoresed on a 4-20% gradient gel under reducing conditions.   Lane 1 shows NAG-1 detection.  Lane 2 shows reactivity is greatly diminished when this antibody is preincubated with the immunizing peptide prior to Western blotting.  Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 (C-term) antibody

WB using Anti-Mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody. Rabbit IgG Ig Fraction Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

WB using Anti-mouse NAG-1/GDF15 antibody. The blot shows detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution. Nag-1 was detected using a 1:40,000 dilution of peroxidase conjugated Gt-a-Rabbit antibody. Rabbit IgG Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

WB shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1,000. For detection, peroxidase conjugated Gt-a-Mouse IgG was used at 1:40000. Mouse 7C12.B3.F2 Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

WB shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1,000. HRP conjugated Gt-a-Mouse IgG secondary antibody was used AT 1:40000. Mouse 23B3D2.H5 Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

WB shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag; lane 3 - yeast cell lysate expressing NAG-1 H variant; lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1000. For detection, HRP conjugated Gt-a-Mouse IgG secondary antibody was used at 1:40000. Mouse 23G10.F8 Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

Predicted band size: 34 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GDF15 antibody

Predicted cell location: Cytoplasm. Positive control: Human renal cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human renal cancer tissue using GDF15 antibody at dilution 1/7, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GDF15 (C-term) antibody

Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

GDF15 (C-term) antibody

Western blot using affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this antibody is pre-incubated with the immunizing peptide prior to Western blotting. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Ms, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

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