GDNF antibody

Principal name

GDNF antibody

Alternative names for GDNF antibody

Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor, ATF

SwissProt ID

P39905 (Human), P48540 (Mouse), Q06PM8 (Xenla), Q07731 (Rat)

Gene ID

2668 (GDNF), 25453 (Gdnf), 14573

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Xen (Xenopus), Eq (Equine)

Available hosts

Goat, Rabbit, Mouse, Sheep, Chicken

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Neutralisation (NEUT)

Background of GDNF antibody

Glial cell-line derived neurotrophic factor (GDNF) is a neurotrophic factor origilly identified for its ability to promote the survival of dopaminergic neurons in the midbrain. GDNF is part of the neurotrophic factor family known as GDNF-family ligand (GFL), which includes neurturin (NRTN), artemin (ARTN), and persephin (PSPN). The GFL ligands are structurally related to the transforming growth factor-b (TGF-b) protein superfamily and contain seven cysteine residues with the same relative spacing as other members of this family. Typical of neurotrophic factors, GDNF is produced in the form of a precursor, preproGDNF, which is then cleaved during secretion to become proGDNF, and later processed to the mature (homodimeric) GDNF form. The GFLs convey their activity by binding to a multicomponent receptor that includes the RET receptor tyrosine kise and a glycosyl phosphatidylinositol (GPI)-linked ligand-binding subunit known as GDNF family receptor a (GFRa). Four different GFRa subunits have been identified (GFRa 1-4) that determine the specificity of the GFRa-RET receptor complex. Thus, GFRa1 together with RET make up the high affinity receptor for GDNF. The GDNF and GFRa complex interaction with RET induces activation of the intracellular tyrosine kise domain of the latter. Phosphorylated RET can then interact with several intracellular sigling cascades including MAP kise, PI3K and PLCg sigling pathways. Lately a RET-independent sigling pathway for GDNF has been shown to involve binding of GDNF and GFRa to the neural cell adhesion molecule (NCAM). Sigling of GDNF via the NCAM complex stimulates intracellular tyrosine kises such as Fyn and FAK. GDNF is synthesized and secreted by a wide variety of cell types in the central and peripheral nervous system where it exerts its neurotrophic effects in the development and maintence of distinct sets of neurons. Accordingly, GDNF knockout mice die shortly after birth, reflecting the central role of GDNF in neurodevelopment.2 Remarkably, GDNF was found to be an essential morphogen for kidney formation as well as to have a role is spermatogenesis.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GDNF antibody

Western blot analysis of recombinant human GDNF (hGDNF): Lanes 1, 4: 50 ng hGDNF. Lanes 2, 5: 10 ng hGDNF. Lanes 3, 4 ng hGDNF. Lanes 1-3: Anti-GDNF antibody , (1:1000). Lanes 4-5: Anti-GDNF antibody preincubated with the control peptide antigen. Rabbit Purified Hu, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

GDNF antibody

To detect hGDNF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hGDNF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

GDNF antibody

To detect hGDNF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GDNF (TA328325) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGDNF. Rabbit IgG Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GDNF antibody

Western blot analysis of human GDNF pro-domain fusion protein at 40 ng (lanes 1 and 4), 30 ng (lanes 2 and 5) and 20ng (lanes 3 and 6): 1-3. Anti-proGDNF antibody, (1:200). 4-6. Anti-proGDNF antibody, preincubated with the control peptide antigen. Note that the expected MW of the GDNF pro-domain fusion protein is 21kD Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

GDNF antibody

Immunohistochemistry testing of anti-GDNF monoclonal antibody 3C1. Analysis was performed using formalin-fixed paraffin-embedded human cerebral cortex tissue sections from Alzheimer’s disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 µg/ml was performed overnight at 4°C. DAKO EnVisionTM Detection System, Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium. A. GDNF staining by monoclonal antibody 3C1; B. Negative staining without primary antibody. Mouse IgG1 3C1 Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

GDNF (N-term) antibody

Western blot analysis of GDNF Antibody (N-term) (AP18033PU-N) in NIH-3T3 cell line lysates (35ug/lane). GDNF (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GDNF antibody

GDNF Rabbit Biotin Hu C, E, WB
25 µg / €230.00
  Acris Antibodies GmbH

GDNF antibody

GDNF Rabbit Biotin Hu C, E, WB
50 µg / €340.00
  Acris Antibodies GmbH

GDNF antibody

GDNF Rabbit Aff - Purified Hu C, E, FN, WB
50 µg / €230.00
  Acris Antibodies GmbH

GDNF antibody

GDNF Rabbit Aff - Purified Hu C, E, FN, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

GDNF antibody

GDNF Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

GDNF antibody

Western Blot analysis of GDNF expression in transfected 293T cell line (H00002668-T01) by GDNF MaxPab polyclonal antibody.

Lane 1: GDNF transfected lysate(23.21 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GDNF antibody

Western Blot analysis of GDNF expression in transfected 293T cell line (H00002668-T02) by GDNF MaxPab polyclonal antibody.

Lane 1: GDNF transfected lysate(23.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

GDNF antibody

GDNF Rabbit Hu, Ms, Rt WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

GDNF antibody

GDNF Rabbit Hu, Ms, Rt C, IP, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

GDNF antibody

Immunohistochemical staining of Glial Derived Neurotrophic Factor ( GDNF ) in rat dorsal root ganglia using GDNF polyclonal antibody ( Cat # PAB14339 ). Sheep Hu, Ms, Rt C, WB
50 µg / €390.00
  Abnova Taiwan Corp.

GDNF antibody

GDNF Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €290.00
  Boster Immunoleader

GDNF antibody

GDNF Goat Aff - Purified Hu, Rt C, E, P, WB
0.1 mg / €450.00
  Neuromics Antibodies

GDNF antibody

GDNF Rabbit IgG Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €470.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GDNF (transcript variant 1)

GDNF Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

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