Geminin antibody

Principal name

Geminin antibody

Alternative names for Geminin antibody

GMNN

SwissProt ID

O75496 (Human), O88513 (Mouse)

Gene ID

51053 (GMNN)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Geminin antibody

Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of geminin fluctuates throughout the cell cycle with geminin levels lowest at G1. Throughout S, G2 and M phases, geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the the degradation of geminin during mitosis and the absence of geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis.

General readings

McGarry TJ. et al (1998) Cell. 93(6):1043-53.
Pitulescu M. et al (2005) Cell Mol Life Sci. 62(13):1425-33.
Luo L. et al (2004) Nature. 427(6976):749-53.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Geminin antibody

Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1 ug/ml] Mouse IgG1 1A8 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Geminin antibody

Predicted band size: 24 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/ 100-500. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Geminin antibody

Western blot analysis of extracts of various cell lines, using GMNN antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Geminin antibody

ELISA: Geminin Antibody Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

Geminin antibody

Western blot analysis The 293T cell lysates(50 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Geminin (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 2H7 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Geminin antibody

Western blot analysis The 293T cell lysates(50 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Geminin (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 2H7 Purified Hu E, WB
50 µl / €230.00
  Acris Antibodies GmbH

Geminin antibody

Western Blot analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody (M02), clone 1B10.

Lane 1: GMNN transfected lysate(23.6 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 1B10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Geminin antibody

Western blot analysis of 293T cell lysates ( 50 µg ) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with GMNN monoclonal antibody , clone 2H7 ( 1 : 500 ) ( Cat # MAB1079 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 2H7 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Geminin antibody

ELISA: Geminin Antibody [33290002] Rabbit Hu E
50 µg / €440.00
  Novus Biologicals Inc.

Geminin antibody

Immunohistochemistry-Paraffin: Geminin Antibody [NB100-40826] - FFPE section of human breast carcinoma.   Affinity purified rabbit anti-Geminin used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu IP, P
0.1 mg / €440.00
  Novus Biologicals Inc.

Geminin antibody

Western Blot and Immunoprecipitation: Geminin Antibody [NB100-40827] -Detection of Human Geminin on HeLa whole cell lysate using NB100-40827. Geminin was also immunoprecipitated by another rabbit anti-Geminin antibodies and NB100-40826. Rabbit Aff - Purified Hu IP, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Geminin antibody

Geminin Mouse IgG1 2H7 Purified Hu E, WB
0.1 ml / €380.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Geminin

Geminin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Geminin (His-tag)

Geminin Human Purified > 95 % >/= 95% by SDS PAGE E. coli
0.5 mg / €990.00
  Acris Antibodies GmbH

Geminin (His-tag)

Geminin Human Purified > 95 % >/= 95% by SDS PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

Geminin

Geminin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Geminin

Geminin Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Geminin

Geminin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Geminin

Geminin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Geminin

SDS-Page: Geminin Protein [NBC1-18423] - Geminin, 27.6kDa (245aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
0.1 mg / €440.00
  Novus Biologicals Inc.

20 Item(s)

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