GET4 antibody

Principal name

GET4 antibody

Alternative names for GET4 antibody

C7orf20, CEE, CGI-20, TRC35, Transmembrane domain recognition complex 35 kDa subunit, Conserved edge-expressed protein, Golgi to ER traffic protein 4 homolog

SwissProt ID

Q0P5I8 (Bovin), Q5ZKG8 (Chick), Q7L5D6 (Human), Q9D1H7 (Mouse)

Gene ID

51608 (GET4)

Available reactivities

Hu (Human), GP (Guinea Pig), Ms (Mouse), Rt (Rat), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of GET4 antibody

GET4 is a component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.

General readings

Mariappan M., Li X., et al. (2010) Nature 466:1120-1124.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GET4 antibody

Host: Rabbit; Target Name: GET4; Sample Tissue: COLO205 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified GP, Hu, Ms, Rt, Ye WB
50 µg / €325.00
  OriGene Technologies, Inc.

GET4 antibody

GET4 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

GET4 antibody

Western Blot: GET4 Antibody [NBP1-41084]-Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-CEE antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  CEE was also immunoprecipitated by another rabbit anti-CEE antibod, which recognizes an upstream epitope Rabbit Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

GET4 antibody

Immunohistochemistry-Paraffin: GET4 Antibody [NBP1-86732] - Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GET4 (full length, C-term DDK tag)

GET4 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

GET4 (1-327, His-tag)

GET4 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

GET4 (1-327, His-tag)

GET4 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

GET4 overexpression lysate

GET4 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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