GFI1B antibody

Principal name

GFI1B antibody

Alternative names for GFI1B antibody

Zinc finger protein Gfi-1b, Growth factor independent protein 1B, Potential regulator of CDKN1A translocated in CML

SwissProt ID

O42409 (Chick), O70237 (Mouse), Q5VTD9 (Human), Q6DCW1 (Xenla)

Gene ID

8328 (GFI1B)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of GFI1B antibody

Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.

General readings

Huang,D.Y., et al., (2004) Nucleic Acids Res. 32 (13), 3935-3946

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GFI1B antibody

Predicted band size: 37 kDa . Positive control: Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GFI1B antibody

GFI1B Antibody (C-term) (Cat. #TA325150) western blot analysis in HL-60,K562,U266 Rabbit IgG Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GFI1B antibody

Human Heart; Rabbit Anti-GFI1B Antibody. Paraffin Embedded Tissue: Human Heart. Cellular Data: Myocardial cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; GFI1B antibody - N-terminal region (AP42015PU-N) in Human Heart cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  Acris Antibodies GmbH

GFI1B antibody

GFI1B Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

GFI1B antibody

Western Blot analysis of GFI1B expression in transfected 293T cell line (H00008328-T01) by GFI1B MaxPab polyclonal antibody.

Lane 1: GFI1B transfected lysate(36.3 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

GFI1B antibody

GFI1B Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GFI1B antibody

Human Heart; Rabbit Anti-GFI1B Antibody. Catalog Number: ARP30093. Paraffin Embedded Tissue: Human Heart. Cellular Data: Myocardial cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; GFI1B antibody - N-terminal region (ARP30093_P050) in Human Heart cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

GFI1B antibody

Immunohistochemistry-Paraffin: GFI1B Antibody [NBP1-86499] Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GFI1B

GFI1B Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

GFI1B

GFI1B Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

GFI1B

GFI1B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GFI1B

GFI1B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GFI1B 293T Cell Transient Overexpression Lysate(Denatured)

GFI1B 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

GFI1B overexpression lysate

GFI1B overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

GFI1B overexpression lysate

GFI1B overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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