GGA1 antibody

Principal name

GGA1 antibody

Alternative names for GGA1 antibody

Gamma-adaptin-related protein 1, ADP-ribosylation factor-binding protein GGA1, Golgi-localized gamma ear-containing ARF-binding protein 1

SwissProt ID

Q8R0H9 (Mouse), Q9UJY5 (Human)

Gene ID

26088 (GGA1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of GGA1 antibody

The GGA family of proteins (Golgi-localized, ARF-binding proteins) are ubiquitous coat proteins that facilitate the trafficking of soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes by means of interactions with TGN-sorting receptors, ARF (ADP-ribosylation factor), and clathrin (1?). Members of the GGA family, GGA1,GGA2 (also known as VEAR) and GGA3, are multidomain proteins that bind mannose 6-phosphate receptors (MPRs) (1,2,4). GGAs have modular structures with an N-terminal VHS (VPS-27, Hrs, and STAM) domain followed by a GAT (GGA and TOM1) domain, a connecting hinge segment, and a C-terminal GAE (?adaptin ear) domain (5). The amino-terminal VHS domains of GGAs form complexes with the cytoplasmic domains of sorting receptors by recognizing acidic-cluster di-leucine (ACLL) sequences (3). GGA1 and GGA2 do not associate with each other, but they do colocalize on perinuclear membranes (2). The cytosolic domain of memapsin 2, but not that of memapsin 1, binds the VHS domains of GGA1 and GGA2 (6). The human GGA1 gene maps to chromosome 22 and encodes a protein that shares 45% sequence identity with GGA2 and GGA3 (1).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GGA1 (336-352) antibody

WB using Anti-GGA1 antibody shows detection of bands at ~100 kDa corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells overexpressing the recombinant protein (arrowhead). Approximately 35 µg of lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4? followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L]. Rabbit IgG Aff - Purified Bov, Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GGA1 (N-term) antibody

Western blot analysis using anti-GGA1 (N-term) Pab (Cat.#AP12154PU-N) to detect GGA1 in mouse kidney tissue lysate (lane 1) and Jurkat cell lysate (lane 2). Rabbit Ig Purified Hu, Ms ICC/IF, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GGA1 antibody

GGA1 MaxPab polyclonal antibody. Western Blot analysis of GGA1 expression in human colon. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

GGA1 antibody

GGA1 MaxPab polyclonal antibody. Western Blot analysis of GGA1 expression in human colon. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GGA1 antibody

Western Blot analysis of GGA1 expression in transfected 293T cell line (H00026088-T02) by GGA1 MaxPab polyclonal antibody.

Lane 1: GGA1 transfected lysate(61.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

GGA1 antibody

Immunoperoxidase of monoclonal antibody to GGA1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml] Mouse IgG 3F11 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GGA1 antibody

Western Blot analysis of GGA1 expression in transfected 293T cell line by GGA1 monoclonal antibody (M02), clone 1G8.

Lane 1: GGA1 transfected lysate(61.4 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 1G8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GGA1 antibody

The GGA1 polyclonal antibody ( Cat # PAB4281 ) is used in Western blot to detect GGA1 in mouse kidney tissue lysate . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

GGA1 antibody

Western blot using GGA1 polyclonal antibody ( Cat # PAB10213 ) shows detection ofbands at ~100 kDa corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells over- expressing the recombinant protein ( arrowhead ).
Approximately 35 µg of lysate was separated on a 4-20% gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,350.
Reaction occurred overnight at 4°C followed by washes and reactionwith a 1 : 10,000 dilution of IRDyeTM800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. Bov, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

GGA1 antibody

GGA1 Mouse IgG1 IMD-20 Ascites Hu, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

GGA1 antibody

GGA1 Rabbit Serum Hu, Ms, Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GGA1 (transcript variant 2)

GGA1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

GGA1

GGA1 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

GGA1 (Human) Matched Antibody Pair

GGA1 (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GGA1 293T Cell Transient Overexpression Lysate(Denatured)

GGA1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

GGA1 overexpression lysate

GGA1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

GGA1 overexpression lysate

GGA1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

GGA1 overexpression lysate

GGA1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

18 Item(s)

per page
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