GGA3 antibody

Principal name

GGA3 antibody

Alternative names for GGA3 antibody

KIAA0154, ADP-ribosylation factor-binding protein GGA3, Golgi-localized gamma ear-containing ARF-binding protein 3

SwissProt ID

Q8BMI3 (Mouse), Q9NZ52 (Human)

Gene ID

23163 (GGA3)

Available reactivities

Hu (Human), Macaque, Mky (Monkey), Ms (Mouse), Rt (Rat), Bt (Bat), Can (Canine), Chk (Chicken), Eq (Equine), Hst (Hamster), Op (Opossum), Por (Porcine), Prim (Primate), Rb (Rabbit), Xen (Xenopus), Tr (Turkey), Bear, Chimp (Chimpanzee), Orang-Utan, Bov (Bovine), GP (Guinea Pig), Ze (Zebrafish)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of GGA3 antibody

GGA3 (also known as ADP-ribosylation factor binding protein GGA3, Golgi-localized, gamma ear-containing, ARF-binding protein 3, Gamma-adaptin related protein 3) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200, GGA1 and GGA2 as well as RABEP1, RABGEF1 and type-I membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. GGA3 is a ubiquitously expressed membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GGA3 (400-415) antibody

WB using Anti-GGA3 antibody shows detection of a band at ~110 kDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cells over- expressing the recombinant protein (lane 2, arrowhead). Pre-incubation of antibody with immunizing peptide blocks specific staining (lane 3). MW markers are shown in lane 1 (700 nm channel - red).The primary antibody was diluted to 1:600. Rabbit IgG Aff - Purified Chimp, Hu, Macaque, Orang-Utan E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GGA3 antibody

WB Suggested Anti-GGA3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GGA3 (400-415) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Breast. Rabbit Aff - Purified Hu, Macaque, Mky E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GGA3 (702-713) antibody

Human Kidney (formalin-fixed, paraffin-embedded) stained with GGA3 antibody AP22476PU-N at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr, Xen E, P
50 µg / €470.00
  Acris Antibodies GmbH

GGA3 antibody

Western Blot analysis of GGA3 expression in transfected 293T cell line (H00023163-T01) by GGA3 MaxPab polyclonal antibody.

Lane 1: GGA3 transfected lysate(74.50 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

GGA3 antibody

GGA3 Goat Hu, Ms, Rt E
0.1 mg / €390.00
  Abnova Taiwan Corp.

GGA3 antibody

Western blot using GGA3 polyclonal antibody ( Cat # PAB10050 ) shows detection of aband at ~110 kDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cellsover- expressing the recombinant protein  (lane 2, arrowhead ).
Pre-incubation of antibody with immunizing peptide blocks specific staining ( lane3 ).
MW markers are shown in lane 1 ( 700 nm channel, red ).
Approximately 35 µg of lysate was separated on a 16% Tricine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:600.
Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature ( 800 nm channel, green ).
IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR.
IRDye is a trademark of LI-COR, Inc. Hu, Macaque
0.1 mg / €560.00
  Abnova Taiwan Corp.

GGA3 antibody

Human Thymus; WB Suggested Anti-GGA3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Thymus; GGA3 antibody - N-terminal region (ARP55423_P050) in Human Thymus cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GGA3 (transcript variant long)

GGA3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

GGA3

GGA3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

GGA3

GGA3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

GGA3

GGA3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GGA3

GGA3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GGA3 293T Cell Transient Overexpression Lysate(Denatured)

GGA3 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

GGA3 overexpression lysate

GGA3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

GGA3 overexpression lysate

GGA3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GGA3 (mGFP-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, GGA3 (mGFP-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,240.00
  OriGene Technologies, Inc.

Lenti ORF particles, GGA3 (mGFP-tagged) - Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant long, 200 uL, >10^7 TU/mL

Lenti ORF particles, GGA3 (mGFP-tagged) - Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant long, 200 uL, >10^7 TU/mL  
200 µl / €1,600.00
  OriGene Technologies, Inc.

Lenti ORF particles, GGA3 (mGFP-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant short, 200 uL, >10^7 TU/mL

Lenti ORF particles, GGA3 (mGFP-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant short, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, GGA3 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, GGA3 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,110.00
  OriGene Technologies, Inc.

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