Ghrelin / GHRL antibody

Principal name

Ghrelin / GHRL antibody

Alternative names for Ghrelin / GHRL antibody

Appetite-regulating hormone, Growth hormone-releasing peptide, Ghrelin-27, Ghrelin-28, Obestatin, Motilin-related peptide, MTLRP, Growth hormone secretagogue, M46 protein

SwissProt ID

Q9BDJ6 (Bovin), Q9BEF8 (Canfa), Q9EQX0 (Mouse), Q9GKY5 (Pig), Q9QYH7 (Rat), Q9UBU3 (Human)

Gene ID

51738 (GHRL), 59301 (Ghrl), 58991

Available reactivities

Hu (Human), Rt (Rat), Hst (Hamster), Mky (Monkey), Ms (Mouse), Por (Porcine), Prim (Primate), Fe (Feline), Bear

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Radioimmunoassay (R), Frozen Sections (C), Immunohistochemistry (IHC), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Ghrelin / GHRL antibody

Ghrelin was originally discovered as a preprohormone that stimulates the release of growth hormone from the anterior pituitary. Recent studies have shown that ghrelin, in concert with other hormones, helps to control both appetite and energy balance. The synthesis of Ghrelin takes place predominantly in epithelial cells of the stomach, although it is also produced in smaller quantities in the placenta, kidney, pituitary and hypothalamus.
Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release from the pituitary. It has an appetite stimulating effect, induces adiposity, and stimulates gastric acid secretion, and it is involved in growth regulation. Ghrelin is synthesized as a preprohormone (13kDa), then proteolytically processed to yield a 28 amino acid peptide (3kDa). Obestatin, an appetite suppressing peptide hormone, is also produced in the process. An interesting and unique modification is imposed on the ghrelin hormone during synthesis in the form of octanoic acid bound to one of its amino acids. This modification is necessary for biological activity.

General readings

1. Stoyanova II, Wiertz RW, Rutten WL. Time-dependent changes in ghrelin-immunoreactivity in dissociated neuronal cultures of the newborn rat neocortex. Regul Pept. 2009 Nov 27;158(1-3):86-90.
PMID: 19540881

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Ghrelin / GHRL (C-term) antibody

  Goat Anti-Ghrelin preproprotein Antibody  
TA305679 (2ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Hu, Rt E, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

Ghrelin / GHRL (N-term) antibody

Immunohistochemistry on paraffin sections: Swine stomach, stained with AM26736PU-N. Mouse IgG1 1ML-1D7 Purified Hu, Por C, P
0.1 mg / €330.00
  Acris Antibodies GmbH

Ghrelin / GHRL (1-118) antibody

Human Colon, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 2F4 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Ghrelin / GHRL (1-118) antibody

Human Colon, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 4B8 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Ghrelin / GHRL (103-117) antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with GHRL antibody AP22499PU-N at 2 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Elephant, Hst, Hu, Mky, Ms, Por, Prim, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

Ghrelin / GHRL (N-term) antibody

Human Pancreas (formalin-fixed, paraffin-embedded) stained with GHRL antibody AP22818PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Bear, Fe, Hu, Mky, Por P
50 µg / €470.00
  Acris Antibodies GmbH

Ghrelin / GHRL antibody

Ghrelin / GHRL Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Ghrelin / GHRL antibody

Ghrelin / GHRL Mouse IgG3 Ghr2 Purified Hu E
1 mg / €510.00
  HyTest Ltd.

Ghrelin / GHRL antibody

Ghrelin / GHRL Mouse IgG2a Ghr5 Purified Hu E
1 mg / €510.00
  HyTest Ltd.

Ghrelin / GHRL antibody

Ghrelin / GHRL Mouse IgG2b Ghr6 Purified Hu E
1 mg / €510.00
  HyTest Ltd.

CRISPR

Catalog No. Species Donor Vector  

Ghrl - mouse gene knockout kit via CRISPR

Ghrl - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ghrl - mouse gene knockout kit via CRISPR

Ghrl - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ghrl - mouse gene knockout kit via CRISPR

Ghrl - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Ghrelin / GHRL (transcript variant 1)

  Purified recombinant protein of Homo sapiens ghrelin/obestatin prepropeptide (GHRL), transcript variant 1  
Ghrelin / GHRL Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Ghrelin / GHRL (24-117, His-tag)

Recombinant human Ghrelin (24-117 aa), His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

Ghrelin / GHRL (24-117, His-tag)

Recombinant human Ghrelin (24-117 aa), His-tagged: 15% SDS-PAGE (3 µg) Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

GHRL overexpression lysate

GHRL overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GHRL (mGFP-tagged) - Human ghrelin/obestatin prepropeptide (GHRL), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, GHRL (mGFP-tagged) - Human ghrelin/obestatin prepropeptide (GHRL), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, GHRL (mGFP-tagged)-Human ghrelin/obestatin prepropeptide (GHRL), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, GHRL (mGFP-tagged)-Human ghrelin/obestatin prepropeptide (GHRL), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, GHRL (mGFP-tagged)-Human ghrelin/obestatin prepropeptide (GHRL), transcript variant 5, 200 uL, >10^7 TU/mL

Lenti ORF particles, GHRL (mGFP-tagged)-Human ghrelin/obestatin prepropeptide (GHRL), transcript variant 5, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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