GIT1 antibody

Principal name

GIT1 antibody

Alternative names for GIT1 antibody

GRK-interacting protein 1, CAT1, ARF GTPase-activating protein GIT1, G protein-coupled receptor kinase-interactor 1, Cool-associated and tyrosine-phosphorylated protein 1

SwissProt ID

Q68FF6 (Mouse), Q9Y2X7 (Human), Q9Z272 (Rat)

Gene ID

28964 (GIT1), 83709 (Git1)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Dot blot (Dot), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of GIT1 antibody

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity . The N-termil two thirds of hsp70s are more conserved than the C-termil third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides . When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-termil fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the Ctermil half . The structure of this ATP binding domain displays multiple features of nucleotide binding proteins .All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to scent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformatiol change leading to the release of the bound substrate protein . The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GIT1 antibody

Adult rat brain membrane immunoblot Mouse IgG1 N39B/8 Supernatant Hu, Ms, Rt C, IP, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

GIT1 antibody

Adult rat hippocampus immunohistochemistry Mouse IgG1 N39B/8 Purified Hu, Ms, Rt C, IP, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

GIT1 antibody

GIT1 Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GIT1 (375-770) antibody

Western blot of rat brain membrane lysates showing the detection of ~90 kDa GIT1 protein using Anti-GIT1 antibody [S39B-8] (TA326533).??Lane 1: MW ladder. Lane 2: Anti-GIT1 (1:1000). Lane 3: Negative control. Load: 33 µ RBM per lane. Block: 5% milk + TBST Mouse IgG1 S39B-8 Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GIT1 antibody

Host: Rabbit; Target Name: Git1; Sample Tissue: Rat Pancreas lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GIT1 pTyr554 antibody

Dot blot analysis of anti-Phospho-GIT1 pTyr554 Antibody (Cat.#AP12965PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

GIT1 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GIT1 Antibody (Y510), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GIT1 pTyr554 antibody

Dot blot analysis of GIT1 (phospho Y554) polyclonal antibody ( Cat # PAB8161 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml. Rabbit Hu Dot, E
0.4 ml / €400.00
  Abnova Taiwan Corp.

GIT1 antibody

Immunohistochemical analysis of GIT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

GIT1 antibody

Immunohistochemical analysis of GIT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

GIT1 antibody

Immunohistochemical analysis of GIT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

GIT1 antibody

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-19141] - FFPE section of human breast carcinoma.  Antibody: Affinity purified rabbit anti-GIT1 used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit IgG Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

GIT1 antibody

Immunohistochemistry: GIT1 Antibody [NBP1-31447] - Immunohistochemical analysis of paraffin-embedded mixed OVCA , using NBP1-31447 at 1:100 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

GIT1 antibody

Immunohistochemistry-Paraffin: GIT1 Antibody [NBP1-85798] - Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GIT1 (transcript variant 2)

GIT1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

GIT1 overexpression lysate

GIT1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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