GJA1 / Cx43 antibody

Principal name

GJA1 / Cx43 antibody

Alternative names for GJA1 / Cx43 antibody

Gap junction alpha-1 protein, Connexin-43, GJAL, Gap junction 43 kDa heart protein

SwissProt ID

P08050 (Rat), P14154 (Chick), P17302 (Human), P18246 (Bovin), P23242 (Mouse), Q29101 (Pig), Q6S9C0 (Canfa), Q6TYA7 (Rabit)

Gene ID

2697 (GJA1), 24392 (Gja1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Hst (Hamster), Av (Birds), Chk (Chicken), Mky (Monkey), Sh (Sheep), Xen (Xenopus), Ze (Zebrafish), GP (Guinea Pig), Vertebrates, Eq (Equine), Bt (Bat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of GJA1 / Cx43 antibody

Gap junctions are aggregations of intercellular channels, which directly connect the cytoplasm of adjacent cells. Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells, synchronization of cellular physiological activities, growth control, and developmental regulation. The gap junction channels allow intercellular exchange of ions, nucleotides, and small molecules between adjacent cells. Unlike other membrane channels, intercellular channels span two plasma membranes and require the contribution of hemi-channels, called connexons, from both participating cells. These channels accommodate molecules as large as 1 kDa. They have been detected in virtually every cell type in mammals, except mature skeletal muscle, spermatozoa, and erythrocytes. Two connexons interact in the extracellular space to form the complete intercellular channel. Each connexon is composed of six similar or identical proteins, which have been termed connexins. Connexins (Cx) are a multi-gene family of highly related proteins with molecular weights between 26 and 70 kDa. At least a dozen distinct connexin genes have been identified; many of them expressed in a tissue-specific manner. Two distinct lineages have been identified in mammals. One termed class I or beta group consists of Cx26, Cx30, Cx31, Cx31.1, and Cx32. The other, termed class II or alpha group, is represented by Cx33, Cx37, Cx40, Cx43 and Cx46. All of the connexins share a common membrane topology but differ in their conductance and channel gating properties. The structure of connexin molecules includes a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops, and a C-terminal cytoplasmic tail of varying length. The various connexins are highly conserved in the transmembrane and extracellular regions, but they differ in their cytoplasmic domain. The 43 kDa connexin protein (connexin-43, Cx43) belongs to the alpha type (group II) subfamily of connexin proteins. It is expressed in most tissues, even though the pattern of expression may differ in various cell types. For example, in the brain it is found in astrocytes, ependyma and leptomeninges but not in neurons, oligodendrocytes and pinealocytes; in the liver it is present in Ito cells but not hepatocytes. Gap junction protein levels change in response to disruption of tissue architecture. For instance, an increased expression of Cx43 was found in early stages of atherosclerosis. Changes in the levels or types of connexin expressed in a given cell type have been found to correlate with tumor progression and metastasis. However, glioma cells transfected with the oncogene neu (c-erb-B2) have been shown to exhibit a major reduction in intercellular communication with no decrease in overall expression of Cx43. Monoclonal antibodies reacting specifically with Cx43 may be applied in diverse cellular and molecular approaches to study gap junctions and their properties. They can also be used to correlate their expression pattern with physiological functions or pathological conditions.

General readings

Joell L. Solan1, et al. (2003) Cell Science 116: 2203-2211 Satoshi Matsushita, et al. (2006) Histochemistry and Cytochemistry 54 (3): 343-353, Xiaoyong Bao, et al. (2004) Cell Physiol 286: C647-C654 W. E. I. Li, et al.(1998) European Journal of Neuroscience 10: 2444

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GJA1 / Cx43 antibody

Western blot analysis of mouse brain membranes: 1.  Anti-Connexin-43 antibody, (1:400).2.  Anti-Connexin-43 antibody, preincubated with the control peptide antigen. Rabbit Purified Ms, Rt WB
50 µl / €325.00
  OriGene Technologies, Inc.

GJA1 / Cx43 antibody

Rat cardiac; . Lanes: Lane1: 8 ug total cardiac lysate. Lane2: 15 ug total cardiac lysate. Lane3: 30 ug total cardiac lysate . Lane4: 50 ug total cardiac lysate. Primary Antibody Dilution: 0.25 ug/ml. Secondary Antibody: . Secondary Antibody Dilution: . Gene Name: GJA1. Submitted by: Anonymous.; GJA1 antibody - N-terminal region (ARP36598_P050) in Rat cardiac cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

GJA1 / Cx43 antibody

Human heart stained with Anti-Connexin 43 antibody Rabbit Purified Hu, Ms, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

GJA1 / Cx43 antibody

Human heart stained with Anti-Connexin 43 antibody Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €520.00
  Acris Antibodies GmbH

GJA1 / Cx43 antibody

Human heart stained with Anti-Connexin 43 antibody Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

GJA1 / Cx43 antibody

TA305614 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GJA1 / Cx43 (C-term) antibody

Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k connexin43 protein. Rabbit IgG Aff - Purified Can, Chk, Finch, Hu, Ms, non-Human Primates, Rt, Sh, Xen, Ze WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GJA1 / Cx43 antibody

Predicted band size: 43 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GJA1 / Cx43 antibody

Western blot analysis of Connexin 43 expression in PMA treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GJA1 / Cx43 antibody

Western blot analysis on HuvEc cell lysate using Connexin 43 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GJA1 / Cx43 (C-term) antibody

Anti-GJA1 / Connexin 43 antibody IHC of human testis, seminiferous tubules and Leydig cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu P
50 µg / €379.00
  OriGene Technologies, Inc.

GJA1 / Cx43 antibody

Anti-GJA1 / Connexin 43 antibody IHC of human seminiferous tubule and Leydig cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Bov, Bt, Can, Eq, Hst, Hu, Mky, Ms, Por, Rb, Rt, Xen
50 µg / €379.00
  OriGene Technologies, Inc.

GJA1 / Cx43 antibody

Western blot analysis of Connexin 43 expression in K562 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GJA1 / Cx43 (C-term) antibody

GJA1 / Cx43 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
20 µl / €50.00
  SICGEN

GJA1 / Cx43 (C-term) antibody

GJA1 / Cx43 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
0.8 mg / €320.00
  SICGEN

GJA1 / Cx43 (C-term) antibody

GJA1 / Cx43 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
20 µl / €50.00
  SICGEN

GJA1 / Cx43 (C-term) antibody

GJA1 / Cx43 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
0.8 mg / €320.00
  SICGEN

GJA1 / Cx43 (C-term) antibody

GJA1 / Cx43 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt C, ICC/IF, P, WB
2.0 mg / €450.00
  SICGEN

GJA1 / Cx43 pSer368 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin43 antibody (AP02508PU). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

GJA1 / Cx43 pSer368 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Connexin43 antibody (AP02508PU). Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µl / €240.00
  Acris Antibodies GmbH

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