GLI1 antibody

Principal name

GLI1 antibody

Alternative names for GLI1 antibody

GLI-1, GLI, Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI

SwissProt ID

P08151 (Human), P47806 (Mouse)

Gene ID

2735 (GLI1), 14632 (Gli1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)

Background of GLI1 antibody

GLI1 is a protein origilly isolated in human glioblastoma. The GLI proteins are the effectors of Sonic Hedgehog (Hh) sigling and have been shown to be involved in cell-fate determition, proliferation and patterning in many cell types and most organs during embryo development. GLI1 was origilly isolated from a glioma tumour and has been found to be up regulated in many tumors including muscle, brain and skin tumors such as Basal cell carcinoma (BCC). Shh and the Gli genes are normally expressed in hair follicles, and skin tumours expressing GLI1 may arise from hair follicles. The level of GLI1 expression correlates with the tumor grade in bone and soft tissue sarcomas. GLI1 expression can be cytoplasm and or nucleus. The GLI1 protein is tethered in the cytoplasm by binding to SUFU. Activation and translocation of the GLI1 protein to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is also promoted by interaction with ZIC1.

General readings

Rahnama,F., et al., (2006) Biochem. J. 394 (PT 1), 19-26

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GLI1 antibody

  GLI1 mouse monoclonal antibody,clone UMAB170  
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-GLI1 mouse monoclonal antibody. (UM800063; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB170 Purified Hu P
0.1 ml / €379.00
  OriGene Technologies, Inc.

UltraMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody,clone UMAB170  
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-GLI1 mouse monoclonal antibody. (UM800063; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min) Mouse IgG1 UMAB170 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2)  
Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803967) Mouse IgG1 OTI4E2 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI2E1 (formerly 2E1)  
Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803969) Mouse IgG1 OTI2E1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  Rabbit Polyclonal Gli1 Antibody  
Western Blot: Gli1 Antibody - WB analysis of Gli-1 on partial recombinant Gli-1 protein (molecular weight of partial recombinant protein is 37 kDa). Rabbit Purified Hu, Ms
0.1 ml / €325.00
  OriGene Technologies, Inc.

GLI1 antibody

  Rabbit Polyclonal Gli1 Antibody  
Immunofluorescence: Gli1 Antibody - IHC analysis of Gli1 in mouse brain using DAB with hematoxylin counterstain. Rabbit Purified Hu, Ms C, E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GLI1 antibody

  Rabbit Polyclonal anti-Gli-1 Antibody  
HepG2 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with purified rabbit polyclonal anti-Gli-1 antibody (poly6424). Proteins were visualized by HRP-conjugated anti-rabbit secondary antibody and a chemiluminescence system. Rabbit IgG Aff - Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

GLI1 antibody

  Rabbit Polyclonal GLI-1 Antibody  
Western Blot: Gli1 Antibody [TA337026] - analysis of human kidney lysate using 1) pre-immune sera at 1:5000 dilution and 2) GLI1 antibody at 1 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Rabbit Purified Hu, Prim ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1. Mouse IgG1 OTI1B4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI2B9 (formerly 2B9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1. Mouse IgG2a OTI2B9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI2G1 (formerly 2G1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1. Mouse IgG1 OTI2G1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  Purified GLI1 mouse monoclonal antibody, clone OTI2D5 (formerly 2D5)  
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803697) Mouse IgG1 OTI2D5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI1B5 (formerly 1B5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1. Mouse IgG1 OTI1B5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1. Mouse IgG2a OTI1H5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI1B1 (formerly 1B1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1. Mouse IgG1 OTI1B1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1. Mouse IgG1 OTI1A8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI2E5 (formerly 2E5)  
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-GLI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803807) Mouse IgG1 OTI2E5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI1E5 (formerly 1E5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1. Mouse IgG1 OTI1E5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI4C8 (formerly 4C8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1. Mouse IgG1 OTI4C8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI1 antibody

  GLI1 mouse monoclonal antibody, clone OTI1F1 (formerly 1F1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1 (RC201110, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI1. Mouse IgG1 OTI1F1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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