GLI2 antibody

Principal name

GLI2 antibody

Alternative names for GLI2 antibody

GLI-2, THP, Zinc finger protein GLI2, Tax helper protein

SwissProt ID

P10070 (Human), P55879, Q0VGT2

Gene ID

2736 (GLI2), 14633 (Gli2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chimp (Chimpanzee)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of GLI2 antibody

Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling, and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes: Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, pre-axial polydactyly type IV, post-axial polydactyly types A1 and B. Expression has been reported for this mRNA in human testis, myometrium, kidney, lung, glioblastomas, and embryonal cell carcinomas. Multiple splice variants have been reported for this protein.

General readings

Ikram,M.S., et al., (2004) J. Invest. Dermatol. 122 (6), 1503-1509

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GLI2 antibody

  Rabbit Polyclonal anti-GLI2 antibody  
Host: Rabbit; Target Name: GLI2; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GLI2 antibody

  Rabbit polyclonal anti-Gli-2 antibody  
WB using Anti-Gli-2 antibody shows detection of a predominant band at ~190 kDa corresponding to Gli-2 (arrowhead) in mouse brain whole cell lysate (lane 1). Pre-incubation of antibody with immunizing peptide completely blocks staining of this band (lane 2). The primary antibody was diluted to 1:750. Incubation was at RT for 2 h followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10  for 45 min at room temperature. Rabbit IgG Aff - Purified Ms, Rt E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GLI2 (40-60) antibody

  Rabbit polyclonal anti-Gli-2 antibody  
WB using Anti-Gli-2 antibody shows detection of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). Each lane contains approximately 35 µg of lysate.  Primary antibody was used at a 1:400 dilution.  The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG [H&L] MX10  for 45 min at RT.   Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Rabbit IgG Aff - Purified Bov, Chimp, Hu, Ms, Rt E, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GLI2 antibody

  Rabbit Polyclonal GLI-2 Antibody  
Immunohistochemistry: Gli2 Antibody [TA336950] - IHC analysis of Gli2 in mouse heart. Rabbit Purified Hu, Ms C, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GLI2 antibody

  GLI2 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)  
Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-GLI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804549) Mouse IgG1 OTI1F9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI2 antibody

  GLI2 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (RC217291, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2. Mouse IgG1 OTI1B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI2 antibody

  GLI2 mouse monoclonal antibody, clone OTI1G2 (formerly 1G2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (RC217291, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2. Mouse IgG1 OTI1G2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI2 antibody

  GLI2 mouse monoclonal antibody, clone OTI1G1 (formerly 1G1)  
Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-GLI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804601) Mouse IgG1 OTI1G1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GLI2 antibody

Figure 1. Staining GLI2 in Mouse Testis by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GLI2 (46-60) (Isoform alpha) antibody

Skin: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu C, E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

GLI2 - human gene knockout kit via CRISPR

GLI2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GLI2 - human gene knockout kit via CRISPR

GLI2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gli2 - mouse gene knockout kit via CRISPR

Gli2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Gli2 - mouse gene knockout kit via CRISPR

Gli2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gli2 - mouse gene knockout kit via CRISPR

Gli2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gli2 - mouse gene knockout kit via CRISPR

Gli2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GLI2 (mGFP-tagged) - Human GLI family zinc finger 2 (GLI2), 200 uL, >10^7 TU/mL

Lenti ORF particles, GLI2 (mGFP-tagged) - Human GLI family zinc finger 2 (GLI2), 200 uL, >10^7 TU/mL  
200 µl / €2,530.00
  OriGene Technologies, Inc.

Lenti ORF particles, GLI2 (Myc-DDK tagged) - Human GLI family zinc finger 2 (GLI2) , 200 uL, >10^7 TU/mL

Lenti ORF particles, GLI2 (Myc-DDK tagged) - Human GLI family zinc finger 2 (GLI2) , 200 uL, >10^7 TU/mL  
200 µl / €2,280.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gli2 (GFP-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gli2 (GFP-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2), 200 uL, >10^7 TU/mL  
200 µl / €2,490.00
  OriGene Technologies, Inc.

Lenti ORF particles, Gli2 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gli2 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2), 200 uL, >10^7 TU/mL  
200 µl / €2,250.00
  OriGene Technologies, Inc.

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