GLI3 antibody

Principal name

GLI3 antibody

Alternative names for GLI3 antibody

GLI-3, Zinc finger protein GLI3

SwissProt ID

P10071 (Human), P55879 (Chick), Q61602 (Mouse)

Gene ID

2737 (GLI3), 14634 (Gli3)

Available reactivities

Can (Canine), Chk (Chicken), Frog, Hu (Human), Mky (Monkey), Quail, Rt (Rat), Ms (Mouse), Chimp (Chimpanzee), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of GLI3 antibody

GLI3(Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila.The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.

General readings

1. Barna, M., Pandolfi, P. P., Niswander, L. Gli3 and Plzf cooperate in proximal limb patterning at early
stages of limb development. (Letter) Nature 436: 277-281, 2005.
2. Bose, J., Grotewold, L., Ruther, U. Pallister-Hall syndrome phenotype in mice mutant for Gli3. Hum.
Molec. Genet. 11: 1129-1135, 2002.
3. Craig, D. W., Itty, A., Panganiban, C., Szelinger, S., Kruer, M. C., Sekar, A., Reiman, D., Narayanan,
V., Stephan, D. A., Kerrigan, J. F.Identification of somatic chromosomal abnormalities in
hypothalamic hamartoma tissue at the GLI3 locus. Am. J. Hum. Genet. 82: 366-374, 2008.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GLI3 (41-57) antibody

WB of Rabbit anti-Gli-3 antibody. Lane 1: human brain whole cell lysate. Lane 2: human lung whole cell lysate. Lane 3: human spleen whole cell lysate. Lane 4: mouse brain whole cell lysate. Lane 5: mouse lung whole cell lysate. Load: 20 µg per lane. Primary antibody: Gli-3 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Rabbit IgG Aff - Purified Can, Chimp, Chk, Hu, Mky, Ms, Rt, Xen E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GLI3 antibody

Western Blot: Gli3 Antibody [TA337186] - WB analysis of Gli3 in Hela cell lysate and MEF cell lysate. Rabbit Purified Hu, Ms C, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GLI3 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GLI-3 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GLI3 (41-57) antibody

Figure 1. Staining GLI3 in  Brain, Glioblastoma by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Can, Chk, Frog, Hu, Mky, Quail, Rt E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GLI3 antibody

GLI3 polyclonal antibody (A01), Lot # 051017JC01 Western Blot analysis of GLI3 expression in HL-60 ( Cat # L014V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

GLI3 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between SMAD1 and GLI3. HeLa cells were stained with anti-SMAD1 rabbit purified polyclonal 1:1200 and anti-GLI3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 2C9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GLI3 antibody

GLI3 Mouse IgG2b 1H7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GLI3 antibody

GLI3 Goat Hu, Ms, Rt E
0.1 mg / €390.00
  Abnova Taiwan Corp.

GLI3 antibody

Immunohistochemistry of GLI3 polyclonal antibody ( Cat # PAB10052 ) was used at a 0.625 µg/ml to detect GLI3 in a variety of tissues.
Strong nuclear and smooth muscle staining was noted to be consistent with previously published reports.
Specific staining was noted in tissue from adrenal, brain, glioblastoma, colon, heart, kidney, lung, liver, skeletal muscle, ovary, pancreas, placenta, skin, spleen, stomach, testes, thymus, thyroid, tonsil and uterus.
This image shows GLI3 staining of human glioblastoma.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA. Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

GLI3 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GLI-3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

GLI3 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GLI-3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

GLI3 antibody

GLI3 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

CRISPR

Catalog No. Species Donor Vector  

GLI3 - human gene knockout kit via CRISPR

GLI3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GLI3 - human gene knockout kit via CRISPR

GLI3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GLI3 - human gene knockout kit via CRISPR

GLI3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GLI3 - human gene knockout kit via CRISPR

GLI3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gli3 - mouse gene knockout kit via CRISPR

Gli3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GLI3

GLI3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GLI3

GLI3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GLI3 (mGFP-tagged)-Human GLI family zinc finger 3 (GLI3), 200 uL, >10^7 TU/mL

Lenti ORF particles, GLI3 (mGFP-tagged)-Human GLI family zinc finger 3 (GLI3), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

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