Glucocorticoid receptor antibody

Principal name

Glucocorticoid receptor antibody

Alternative names for Glucocorticoid receptor antibody

NR3C1, GR, GRL

SwissProt ID

P04150 (Human), P06536 (Rat), P06537 (Mouse), Q9N1U3 (Pig)

Gene ID

2908 (NR3C1), 24413 (Nr3c1)

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Rb (Rabbit), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ChIP assay (ChIP), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of Glucocorticoid receptor antibody

Glucocorticoid hormones control cellular proliferation, inflammation, and metabolism through their association with the glucocorticoid receptor (GR)/NR3C1, a member of the nuclear hormone receptor superfamily of transcription factors . GR is composed of several conserved structural elements, including a carboxy-termil ligand-binding domain (which also contains residues critical for receptor dimerization and hormone-dependent gene transactivation), a neighboring hinge region containing nuclear localization sigls, a central zinc-finger-containing D-binding domain, and an amino-termil variable region that participates in ligand-independent gene transcription. In the absence of hormone, a significant population of GR is localized to the cytoplasm in an ictive form via its association with regulatory chaperone proteins, such as HSP90, HSP70, and FKBP52. On hormone binding, GR is released from the chaperone complex and translocates to the nucleus as a dimer to associate with specific D sequences termed glucocorticoid response elements (GREs), thereby enhancing or repressing transcription of specific target genes . It was demonstrated that GR-mediated transcriptiol activation is modulated by phosphorylation. Although GR can be basally phosphorylated in the absence of hormone, it becomes hyperphosphorylated upon binding receptor agonists. It has been suggested that hormone-dependent phosphorylation of GR may determine target promoter specificity, cofactor interaction, strength and duration of receptor sigling, receptor stability, and receptor subcellular localization . Indeed Ser211 of human GR is phosphorylated to a greater extent in the presence of hormone, and biochemical fractiotion studies following hormone treatment indicate that Ser211-phosphorylated GR is found in the nucleus . Thus, Ser211 phosphorylation is a biomarker for activated GR in vivo. An added layer of complexity to GR sigling lies in the ability of multipl

General readings

Encio, I.J., et al., (1996) Clinical Neurogenetics Branch, National Institute of Mental Health, Bldg. 10, Rm. 3N218, Bethesda, MD 20892, USA

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glucocorticoid receptor antibody

  Rabbit polyclonal GR (Ab-211) antibody  
Western blot analysis of extracts from HeLa cells treated with PMA (125ng/ml, 30min), using GR (Ab-211) antibody (#TA313373, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  NR3C1 mouse monoclonal antibody, clone OTI4C9 (formerly 4C9)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806190) Mouse IgG2b OTI4C9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Glucocorticoid receptor antibody

  NR3C1 mouse monoclonal antibody, clone OTI6A12 (formerly 6A12)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806237) Mouse IgG1 OTI6A12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Glucocorticoid receptor antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NR3C1 antibody Cat.-No AM06570SU-N with DAB staining. Mouse IgG1 6E6 Ascites Hu E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Glucocorticoid receptor antibody

Immunofluorescence of monoclonal antibody to NR3C1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2C8 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Glucocorticoid receptor antibody

Immunocytochemistry using the NR3C1 monoclonal antibody, clone m2F8 ( Cat # MAB0119 ). COS-7 cells transiently overexpressing human NR3C1 were labeled with the NR3C1 monoclonal antibody, clone m2F8 ( Cat # MAB0119 ) at 2.5 µg/ml followed by biotinylated second antibody and peroxidase-labeled avidin. Mouse IgG2b m2F8 Hu C, ChIP, E, F, ICC/IF, IP, WB
50 µg / €470.00
  Abnova Taiwan Corp.

Glucocorticoid receptor antibody

  Rabbit Polyclonal antibody to Glucocorticoid Receptor beta (nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor))  
Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. TA308891 diluted at 1:1000. Rabbit IgG Aff - Purified Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Rabbit polyclonal GR (Ab-226) antibody  
Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 15mins), using GR (Ab-226) antibody (#TA313368).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Rabbit polyclonal GR (Ab-203) antibody  
Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using GR (Ab-203) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Rabbit polyclonal GR (Ser211) antibody(Phospho-specific)  
Western blot analysis of extracts from HeLa cells treated with Heat shock, using GR (phospho-Ser211) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Rabbit polyclonal GR (Ser226) antibody(Phospho-specific)  
Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 15mins), using GR (Phospho-Ser226) antibody (#TA313371).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Rabbit polyclonal GR (Ser203) antibody(Phospho-specific)  
Western blot analysis of extracts from A549 cells, treated with dexamethason (10 nM, 1 hour), using GR (Phospho-Ser203) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Mouse monoclonal NR3C1 Antibody  
NR3C1 Antibody (N-term) (Cat. #TA324708) western blot analysis in Jurkat,HepG2,mouse NIH/3T3 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the NR3C1 antibody detected the NR3C1 protein (arrow). Mouse IgM 740CT12.1.5 Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Rabbit polyclonal NR3C1 Antibody (C-term)  
Western blot analysis of NR3C1 Antibody (C-term) (Cat. #TA324793) in A549 cell line lysates (35ug/lane). NR3C1 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Rabbit polyclonal NR3C1 Antibody (Center)  
NR3C1 Antibody (Center) (Cat.#TA324810) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NR3C1 antibody detected NR3C1 protein (arrow). Rabbit IgG Purified Hu, Ms, Rb ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Rabbit Polyclonal GR Antibody  
Western blot analysis of GR expression in PMA treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Rabbit Polyclonal GR Antibody  
Western blot analysis of GR expression in EGF treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Rabbit Polyclonal GR Antibody  
Western blot analysis of GR expression in PMA treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  NR3C1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using NR3C1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

  Mouse Monoclonal GR Antibody  
WB using the antibody against hGR. Figure 4B . WB analysis of extracts from 300,000 HeLa cells with the antibody against hGR (concentration 1 ug/ml). Mouse IgG2b Monoclol Purified Hu E, F
50 µg / €325.00
  OriGene Technologies, Inc.

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