Glucocorticoid receptor antibody

Principal name

Glucocorticoid receptor antibody

Alternative names for Glucocorticoid receptor antibody

NR3C1, GR, GRL

SwissProt ID

P04150 (Human), P06536 (Rat), P06537 (Mouse), Q9N1U3 (Pig)

Gene ID

2908 (NR3C1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), GP (Guinea Pig), Rb (Rabbit), Sh (Sheep), Ye (Yeast), Por (Porcine), Bov (Bovine)

Available hosts

Rabbit, Mouse, Sheep

Available applications

ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F), Electrophoretic Mobility Shift Assay (EMSA), Neutralisation (NEUT), ELISA (detection) (E(detection))

Background of Glucocorticoid receptor antibody

Glucocorticoid hormones control cellular proliferation, inflammation, and metabolism through their association with the glucocorticoid receptor (GR)/NR3C1, a member of the nuclear hormone receptor superfamily of transcription factors . GR is composed of several conserved structural elements, including a carboxy-termil ligand-binding domain (which also contains residues critical for receptor dimerization and hormone-dependent gene transactivation), a neighboring hinge region containing nuclear localization sigls, a central zinc-finger-containing D-binding domain, and an amino-termil variable region that participates in ligand-independent gene transcription. In the absence of hormone, a significant population of GR is localized to the cytoplasm in an ictive form via its association with regulatory chaperone proteins, such as HSP90, HSP70, and FKBP52. On hormone binding, GR is released from the chaperone complex and translocates to the nucleus as a dimer to associate with specific D sequences termed glucocorticoid response elements (GREs), thereby enhancing or repressing transcription of specific target genes . It was demonstrated that GR-mediated transcriptiol activation is modulated by phosphorylation. Although GR can be basally phosphorylated in the absence of hormone, it becomes hyperphosphorylated upon binding receptor agonists. It has been suggested that hormone-dependent phosphorylation of GR may determine target promoter specificity, cofactor interaction, strength and duration of receptor sigling, receptor stability, and receptor subcellular localization . Indeed Ser211 of human GR is phosphorylated to a greater extent in the presence of hormone, and biochemical fractiotion studies following hormone treatment indicate that Ser211-phosphorylated GR is found in the nucleus . Thus, Ser211 phosphorylation is a biomarker for activated GR in vivo. An added layer of complexity to GR sigling lies in the ability of multipl

General readings

This product has been used in:Cidlowski JA et al. Novel antipeptide antibodies to the human glucocorticoid receptor: recognition of multiple receptor forms in vitro and distinct localization of cytoplasmic and nuclear receptors. Mol Endocrinol 4:1427-37 (1990). PubMed PMID: 1704480
General / background references:Whitelaw ML et al. Heat shock protein hsp90 regulates dioxin receptor function in vivo. Proc Natl Acad Sci U S A 92:4437-41 (1995). PubMed PMID: 7753824
van Steensel B et al. Localization of the glucocorticoid receptor in discrete clusters in the cell nucleus. J Cell Sci 108 (Pt 9):3003-11 (1995). PubMed PMID: 8537440
Gaitan D et al. Glucocorticoid receptor structure and function in an adrenocorticotropin-secreting small cell lung cancer. Mol Endocrinol 9:1193-201 (1995). PubMed PMID: 7491111
Whitelaw ML et al. Identification of transactivation and repression functions of the dioxin receptor and its basic helix-loop-helix/PAS partner factor Arnt: inducible versus constitutive modes of regulation. Mol Cell Biol 14:8343-55 (1994). PubMed PMID: 7969169
Trapp T et al. Heterodimerization between mineralocorticoid and glucocorticoid receptor: a new principle of glucocorticoid action in the CNS. Neuron 13:1457-62 (1994). PubMed PMID: 7993637
Bellingham DL et al. Ligand-dependent down-regulation of stably transfected human glucocorticoid receptors is associated with the loss of functional glucocorticoid responsiveness. Mol Endocrinol 6:2090-102 (1992). PubMed PMID: 1491690
Rangarajan PN et al. Modulation of glucocorticoid receptor function by protein kinase A. Mol Endocrinol 6:1451-7 (1992). PubMed PMID: 1435789
Burnstein KL et al. Autoregulation of glucocorticoid receptor gene expression. Steroids 56:52-8 (1991). PubMed PMID: 2020978
McGimsey WC et al. Immunocytochemical localization of the glucocorticoid receptor in rat brain, pituitary, liver, and thymus with two new polyclonal antipeptide antibodies. Endocrinology 129:3064-72 (1991). PubMed PMID: 1720088
Burnstein KL et al. Human glucocorticoid receptor cDNA contains sequences sufficient for receptor down-regulation. J Biol Chem 265:7284-91 (1990). PubMed PMID: 1692020

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glucocorticoid receptor antibody

Western blot analysis of extracts from HeLa cells treated with PMA (125ng/ml, 30min), using GR (Ab-211) antibody (#TA313373, Line 1 and 2).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806190) Mouse IgG2b OTI4C9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Glucocorticoid receptor antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806237) Mouse IgG1 OTI6A12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Glucocorticoid receptor antibody

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NR3C1 antibody Cat.-No AM06570SU-N with DAB staining. Mouse IgG1 6E6 Ascites Hu E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Glucocorticoid receptor antibody

Immunofluorescence of monoclonal antibody to NR3C1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2C8 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Glucocorticoid receptor antibody

Immunocytochemistry using the NR3C1 monoclonal antibody, clone m2F8 ( Cat # MAB0119 ). COS-7 cells transiently overexpressing human NR3C1 were labeled with the NR3C1 monoclonal antibody, clone m2F8 ( Cat # MAB0119 ) at 2.5 µg/ml followed by biotinylated second antibody and peroxidase-labeled avidin. Mouse IgG2b m2F8 Hu C, ChIP, E, F, ICC/IF, IP, WB
50 µg / €470.00
  Abnova Taiwan Corp.

Glucocorticoid receptor antibody

Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. TA308891 diluted at 1:1000. Rabbit IgG Aff - Purified Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 15mins), using GR (Ab-226) antibody (#TA313368).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using GR (Ab-203) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

NR3C1 Antibody (N-term) (Cat. #TA324708) western blot analysis in Jurkat,HepG2,mouse NIH/3T3 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the NR3C1 antibody detected the NR3C1 protein (arrow). Mouse IgM 740CT12.1.5 Purified Hu, Ms WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

Western blot analysis of NR3C1 Antibody (C-term) (Cat. #TA324793) in A549 cell line lysates (35ug/lane). NR3C1 (arrow) was detected using the purified Pab. Rabbit IgG Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

NR3C1 Antibody (Center) (Cat.#TA324810) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NR3C1 antibody detected NR3C1 protein (arrow). Rabbit IgG Purified Hu, Ms, Rb ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

Western blot analysis of GR expression in PMA treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

Western blot analysis of GR expression in EGF treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

Western blot analysis of GR expression in PMA treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

Western blot analysis of extracts of various cell lines, using NR3C1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

WB using the antibody against hGR. Figure 4B . WB analysis of extracts from 300,000 HeLa cells with the antibody against hGR (concentration 1 ug/ml). Mouse IgG2b Monoclol Purified Hu E, F
50 µg / €325.00
  OriGene Technologies, Inc.

Glucocorticoid receptor antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA806187) Mouse IgG2b OTI2C4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Glucocorticoid receptor antibody

Human recombinant protein fragment corresponding to amino acids 1-265 of human NR3C1(NP_000167) produced in E.coli. Mouse IgG1 OTI2E12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Glucocorticoid receptor antibody

Human recombinant protein fragment corresponding to amino acids 1-265 of human NR3C1(NP_000167) produced in E.coli. Mouse IgG1 OTI1B11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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