Glucokinase / Hexokinase-4 antibody

Principal name

Glucokinase / Hexokinase-4 antibody

Alternative names for Glucokinase / Hexokinase-4 antibody

Hexokinase type IV, Hexokinase-D, GCK, HK4, HKIV

SwissProt ID

P17712 (Rat), P35557 (Human), P35557-3, P52792 (Mouse)

Gene ID

2645 (GCK)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F)

Background of Glucokinase / Hexokinase-4 antibody

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq]. Transcript Variant: This variant (3) is the minor form expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3; its second exon is unique to this transcript. Isoform 3 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 2. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Jon E. et al.,(2003) J.Exp Biology. 206 : 2049-2057.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glucokinase / Hexokinase-4 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459) Mouse IgG1 OTI3E3 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Glucokinase / Hexokinase-4 antibody

Immunohistochemistry-Paraffin: IGLK Antibody (3E3) [NBP1-47778] - Immunohistochemical staining of paraffin-embedded breast tissue using anti-GCK mouse monoclonal antibody.  (Dilution 1:50) Mouse 3E3 Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

Glucokinase / Hexokinase-4 antibody

Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using GCK antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucokinase / Hexokinase-4 antibody

Predicted cell location: Cytoplasm. Positive control: Human liver and colon cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human liver and colon cancer tissue using GCK antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucokinase / Hexokinase-4 (N-term) antibody

GCK Antibody (M1) (Cat.# TA325004) western blot analysis in HepG2 cell line and rat liver lysates (35ug/lane).This demonstrates the GCK antibody detected the GCK protein (arrow). Rabbit Ig Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Glucokinase / Hexokinase-4 antibody

Western blot analysis using GCK mAb against HEK293 (1) and GCK-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 4G6 Ascites Hu E, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Glucokinase / Hexokinase-4 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Glucokinase / Hexokinase-4 antibody

GCK MaxPab polyclonal antibody. Western Blot analysis of GCK expression in human pancreas. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Glucokinase / Hexokinase-4 antibody

Immunofluorescence of purified MaxPab antibody to GCK on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Glucokinase / Hexokinase-4 antibody

Western Blot analysis of GCK expression in transfected 293T cell line (H00002645-T01) by GCK MaxPab polyclonal antibody.

Lane 1: GCK transfected lysate(52.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu, Ms, Rt E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Glucokinase / Hexokinase-4 antibody

Detection limit for recombinant GST tagged GCK is approximately 0.1ng/ml as a capture antibody. Mouse IgG1 3C7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Glucokinase / Hexokinase-4 antibody

Detection limit for recombinant GST tagged GCK is approximately 0.03ng/ml as a capture antibody. Mouse IgG1 1G10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Glucokinase / Hexokinase-4 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with GCK polyclonal antibody ( Cat # PAB3531 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Glucokinase / Hexokinase-4 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with GCK polyclonal antibody ( Cat # PAB3532 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Glucokinase / Hexokinase-4 (N-term) antibody

Human COLO205; WB Suggested Anti-GCK Antibody. . Titration: 1.0 ug/ml. . Positive Control: COLO205 Whole Cell; GCK antibody - N-terminal region (ARP61473_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Glucokinase / Hexokinase-4 antibody

Western blot analysis of Glucokinase expression in HEK293T (A) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Glucokinase / Hexokinase-4 antibody

Western blot analysis of Glucokinase expression in HEK293T (A) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Glucokinase / Hexokinase-4 antibody

Western blot analysis of Glucokinase expression in HEK293T (A) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Glucokinase / Hexokinase-4 antibody

Glucokinase / Hexokinase-4 Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Glucokinase / Hexokinase-4 antibody

Glucokinase / Hexokinase-4 Rabbit IgG Aff - Purified Hu E, P, WB
0.1 mg / €550.00
  Novus Biologicals Inc.

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