Glucokinase / Hexokinase-4 antibody

Principal name

Glucokinase / Hexokinase-4 antibody

Alternative names for Glucokinase / Hexokinase-4 antibody

Hexokinase type IV, Hexokinase-D, GCK, HK4, HKIV

SwissProt ID

P17712 (Rat), P35557 (Human), P35557-3, P52792 (Mouse)

Gene ID

2645 (GCK), 103988 (Gck)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair)

Background of Glucokinase / Hexokinase-4 antibody

Kit Component:
- KN306364G1, Gck gRNA vector 1 in pCas-Guide vector
- KN306364G2, Gck gRNA vector 2 in pCas-Guide vector
- KN306364D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Jon E. et al.,(2003) J.Exp Biology. 206 : 2049-2057.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glucokinase / Hexokinase-4 antibody

  GCK mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)  
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GCK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500459) Mouse IgG1 OTI3E3 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Glucokinase / Hexokinase-4 antibody

  Anti-GCK Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using GCK antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucokinase / Hexokinase-4 antibody

  Anti-GCK Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human liver and colon cancer tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human liver and colon cancer tissue using GCK antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucokinase / Hexokinase-4 (N-term) antibody

  Rabbit polyclonal GCK Antibody (N-term)  
GCK Antibody (M1) (Cat.# TA325004) western blot analysis in HepG2 cell line and rat liver lysates (35ug/lane).This demonstrates the GCK antibody detected the GCK protein (arrow). Rabbit Ig Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Glucokinase / Hexokinase-4 antibody

  Rabbit Polyclonal Anti-GCK Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: HT29 cells, Primary antibody: (GCK Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucokinase / Hexokinase-4 antibody

Western blot analysis using GCK mAb against HEK293 (1) and GCK-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 4G6 Ascites Hu E, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Glucokinase / Hexokinase-4 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

GCK - human gene knockout kit via CRISPR

GCK - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GCK - human gene knockout kit via CRISPR

GCK - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GCK - human gene knockout kit via CRISPR

GCK - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gck - mouse gene knockout kit via CRISPR

Gck - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Gck - mouse gene knockout kit via CRISPR

Gck - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gck - mouse gene knockout kit via CRISPR

Gck - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gck - mouse gene knockout kit via CRISPR

Gck - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Glucokinase / Hexokinase-4 (transcript variant 1)

  Recombinant protein of human glucokinase (hexokinase 4) (GCK), transcript variant 1  
Glucokinase / Hexokinase-4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Glucokinase / Hexokinase-4 (transcript variant 3)

  Recombinant protein of human glucokinase (hexokinase 4) (GCK), transcript variant 3  
Glucokinase / Hexokinase-4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Glucokinase / Hexokinase-4 (transcript variant 2)

  Recombinant protein of human glucokinase (hexokinase 4) (GCK), transcript variant 2  
Glucokinase / Hexokinase-4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Glucokinase / Hexokinase-4 (His-tag)

Glucokinase / Hexokinase-4 Human Purified > 95 % >/= 95% by SDS PAGE E. coli
0.5 mg / €1,500.00
  Acris Antibodies GmbH

Glucokinase / Hexokinase-4 (His-tag)

Glucokinase / Hexokinase-4 Human Purified > 95 % >/= 95% by SDS PAGE E. coli
0.1 mg / €520.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

GCK overexpression lysate

GCK overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

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