Glucosidase 2 subunit beta antibody

Principal name

Glucosidase 2 subunit beta antibody

Alternative names for Glucosidase 2 subunit beta antibody

Glucosidase II beta, PRKCSH, G19P1, 80K-H protein, Protein kinase C substrate 60.1 kDa protein heavy chain

SwissProt ID

O08795 (Mouse), P14314 (Human), Q28034 (Bovin)

Gene ID

5589 (PRKCSH), 855899 (AFT2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP), Paraffin Sections (P)

Background of Glucosidase 2 subunit beta antibody

Trimming of glucoses from N-linked core glycans on newly synthesized glycoproteins occurs sequentially through the action of Glucosidases I and II in the endoplasmic reticulum (ER). Glucosidase II is an ER-localized enzyme that contains alpha and beta subunits (Glucosidase IIalpha and Glucosidase IIbeta). The alpha and beta subunits form a defined heterodimeric complex with a molecular weight about 161 kDa. Glucosidase IIalpha is the catalyitc core of the enzyme and can function independently of the beta subunit. The sequence of Glucosidase IIbeta encodes protein rich in glutamic and aspartic acid with a putative ER retention signal (HDEL) at the C terminus. The phosphorylated form of Glucosidase IIbeta is localized in the plasma membrane and is highly expressed in FGF stimulated fibroblasts and epidermal carcinoma cells. Glucosidase IIbeta was first purified from a human carcinoma cell line as a potential substrate for protein kinase C. Through the HDEL signal at the C-terminus, Glucosidase IIbeta retains the complete complex in the ER.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glucosidase 2 subunit beta antibody

Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. TA307924 diluted at 1:10000. Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucosidase 2 subunit beta antibody

Western blot analysis of extracts from mouse brain cells, using GLU2B antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glucosidase 2 subunit beta antibody

Western blot (WB) analysis of Glucosidase II beta antibody (Cat.-No.: AP01229PU-N) in extracts from mouse brain. Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Glucosidase 2 subunit beta antibody

PRKCSH MaxPab polyclonal antibody. Western Blot analysis of PRKCSH expression in human liver. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Glucosidase 2 subunit beta antibody

PRKCSH MaxPab polyclonal antibody. Western Blot analysis of PRKCSH expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Glucosidase 2 subunit beta antibody

Immunoprecipitation of PRKCSH transfected lysate using anti-PRKCSH MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PRKCSH MaxPab mouse polyclonal antibody (B01) (H00005589-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Glucosidase 2 subunit beta antibody

Western Blot analysis of PRKCSH expression in transfected 293T cell line (H00005589-T02) by PRKCSH MaxPab polyclonal antibody.

Lane 1: PRKCSH transfected lysate(45.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Glucosidase 2 subunit beta antibody

Glucosidase 2 subunit beta Mouse IgG2a 3H7 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Glucosidase 2 subunit beta antibody

Immunofluorescence analysis of HeLa cells, using GLU2B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

Glucosidase 2 subunit beta antibody

Immunofluorescence analysis of HeLa cells, using GLU2B Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

Glucosidase 2 subunit beta antibody

Immunofluorescent analysis of Beta-glucosidase 2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Glucosidase 2 subunit beta antibody

Immunofluorescent analysis of Beta-glucosidase 2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Glucosidase 2 subunit beta antibody

Immunofluorescent analysis of Beta-glucosidase 2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Glucosidase 2 subunit beta antibody

Western Blot: Glucosidase IIbeta Antibody [NBP1-00851] - Western blot (WB) analysis   of Glucosidase IIbeta (K117) antibody in extracts from mouse brain. Rabbit Aff - Purified Hu, Ms ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Glucosidase 2 subunit beta antibody

Western Blot: PRKCSH Antibody [NBP1-32006-  (30 ug of whole cell lysate) A: A431  7.5% SDS PAGE  diluted at 1:10000 Rabbit IgG Hu ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Glucosidase 2 subunit beta antibody

Glucosidase 2 subunit beta Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Glucosidase 2 subunit beta antibody

Glucosidase 2 subunit beta Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Glucosidase 2 subunit beta

Glucosidase 2 subunit beta Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

Glucosidase 2 subunit beta

Glucosidase 2 subunit beta Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Glucosidase 2 subunit beta

Glucosidase 2 subunit beta Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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