Glutamate dehydrogenase 1 antibody

Principal name

Glutamate dehydrogenase 1 antibody

Alternative names for Glutamate dehydrogenase 1 antibody

GLUD1, GLUD, GDH1

SwissProt ID

P00366 (Bovin), P00367 (Human), P10860 (Rat), P26443 (Mouse), P42174 (Pig)

Gene ID

2746 (GLUD1), 281785, 4527463 (alr)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog, Can (Canine), Chimp (Chimpanzee), Eq (Equine), Mky (Monkey), Por (Porcine), Gorilla, Sh (Sheep), Ye (Yeast), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Dot blot (Dot), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R)

Background of Glutamate dehydrogenase 1 antibody

L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification. L-glutamate dehydrogenase (EC 1.4.1.3) has a central role in nitrogen metabolism in plants and animals. Glutamate dehydrogenase is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate (Smith et al., 2001 [PubMed 11254391]). Glutamate, the main substrate of GLUD, is present in brain in concentrations higher than in other organs. In nervous tissue, GLUD appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification (Mavrothalassitis et al., 1988 [PubMed 3368458]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-51 M20867.1 1-51 52-3120 BC112946.1 16-3084

General readings

General References:
1. Kim DW, et al. (2003) Molecular gene cloning, expression, and characterization of bovine brain glutamate dehydrogenase. J Biochem Mol Biol. 36(6):545-51.
2. Cho S.W., Lee J., Choi S.Y. (1995) Two soluble forms of glutamate dehydrogenase isoproteins from bovine brain. Eur. J. Biochem. 233:340-346.
3. Lee J., Kim S.W., Cho S.W. (1995) A novel glutamate dehydrogenase from bovine brain: purification and characterization. Biochem. Mol. Biol. Int. 36:1087-1096.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glutamate dehydrogenase 1 antibody

lanes 5: rat kidney cordex; lanes 6: rat kidney proximal tubules prepped from cortex; lanes 7: LLCPK-F+ pig kidney proximal tubule tissue culture lysate; lanes 8: rat brain supernatant Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt, Sh, Ye, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Glutamate dehydrogenase 1 antibody

GLUD1 antibody - N-terminal region validated by WB using Fetal Liver Lysate at 1 ug/ml. Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Glutamate dehydrogenase 1 antibody

Human Pancreas; Immunohistochemistry with pFA fixed human pancreas tissue tissue; GLUD1 antibody - N-terminal region (ARP45708_P050) in Human Pancreas cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

Glutamate dehydrogenase 1 antibody

Human Kidney; Immunohistochemistry with CORTEX/KIDNEY tissue; GLUD1 antibody - N-terminal region (ARP45709_P050) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

Glutamate dehydrogenase 1 (+Glutamate dehydrogenase 2) antibody

AP23724PU-N (3.8 µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 antibody

Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml. Goat Purified Chimp, Eq, Gorilla, Hu, Mky, Ms, Por E
50 µg / €345.00
  OriGene Technologies, Inc.

Glutamate dehydrogenase 1 antibody

Anti-GLUD1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Rabbit IgG Purified Bov, Hu E, P, WB
0.5 mg / €345.00
  OriGene Technologies, Inc.

Glutamate dehydrogenase 1 antibody

Glutamate dehydrogenase 1 Rabbit IgG Azide Free Bov Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €230.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 antibody

Glutamate dehydrogenase 1 Rabbit IgG Biotin Bov Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 antibody

Glutamate dehydrogenase 1 Rabbit IgG Aff - Purified Bov Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 antibody

Liver, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Serum Bov E, P, WB
0.5 ml / €470.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 antibody

Figure 1. Western blot analysis is shown using anti-Bovine Glutamate Dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 µm nitrocellulose. The blot was incubated with a 1:1,000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye(TM) 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature. IRDye(TM) 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit Serum Bov E, WB
2 ml / €1,150.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 antibody

Western Blot: Glutamate Dehydrogenase Antibody [NB600-853] - Western blot analysis is shown using NB600-853 anti-Bovine Glutamate Dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose. The blot was incubated with a 1:1,000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Aff - Purified Bov E, IP, WB
0.2 ml / €250.00
  Novus Biologicals Inc.

Glutamate dehydrogenase 1 antibody

Western Blot: Glutamate Dehydrogenase Antibody [NBP1-54961] - This Anti-GLUD1 antibody was used in Western Blot of HT1080     tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu P, WB
50 µg / €440.00
  Novus Biologicals Inc.

Glutamate dehydrogenase 1 antibody

Glutamate dehydrogenase 1 Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Glutamate dehydrogenase 1

Glutamate dehydrogenase 1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Glutamate dehydrogenase 1 (54-558, His-tag)

Glutamate dehydrogenase 1 Human Purified > 80 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 (54-558, His-tag)

Glutamate dehydrogenase 1 Human Purified > 80 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

GLUD1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)

GLUD1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)  
0.1 mg / €270.00
  Abnova Taiwan Corp.

19 Item(s)

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