Glutamate dehydrogenase 1 antibody

Principal name

Glutamate dehydrogenase 1 antibody

Alternative names for Glutamate dehydrogenase 1 antibody

GLUD1, GLUD, GDH1

SwissProt ID

P00366 (Bovin), P00367 (Human), P10860 (Rat), P26443 (Mouse), P42174 (Pig)

Gene ID

2746 (GLUD1), 281785, 14661 (Glud1), 24399

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Can (Canine), Chimp (Chimpanzee), Eq (Equine), Mky (Monkey), Por (Porcine), Gorilla, Sh (Sheep), Ye (Yeast), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Dot blot (Dot), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R)

Background of Glutamate dehydrogenase 1 antibody

Kit Component:
- KN306554G1, Glud1 gRNA vector 1 in pCas-Guide vector
- KN306554G2, Glud1 gRNA vector 2 in pCas-Guide vector
- KN306554D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

General References:
1. Kim DW, et al. (2003) Molecular gene cloning, expression, and characterization of bovine brain glutamate dehydrogenase. J Biochem Mol Biol. 36(6):545-51.
2. Cho S.W., Lee J., Choi S.Y. (1995) Two soluble forms of glutamate dehydrogenase isoproteins from bovine brain. Eur. J. Biochem. 233:340-346.
3. Lee J., Kim S.W., Cho S.W. (1995) A novel glutamate dehydrogenase from bovine brain: purification and characterization. Biochem. Mol. Biol. Int. 36:1087-1096.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glutamate dehydrogenase 1 antibody

  Rabbit Polyclonal Anti-GLUD1 Antibody  
lanes 5: rat kidney cordex; lanes 6: rat kidney proximal tubules prepped from cortex; lanes 7: LLCPK-F+ pig kidney proximal tubule tissue culture lysate; lanes 8: rat brain supernatant Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt, Sh, Ye, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Glutamate dehydrogenase 1 antibody

  Rabbit Polyclonal Anti-GLUD1 Antibody  
GLUD1 antibody - N-terminal region validated by WB using Fetal Liver Lysate at 1 ug/ml. Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Glutamate dehydrogenase 1 (+Glutamate dehydrogenase 2) antibody

AP23724PU-N (3.8 µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 antibody

  GLUD1 Goat Polyclonal (Internal) Antibody  
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml. Goat Purified Chimp, Eq, Gorilla, Hu, Mky, Ms, Por E
50 µg / €400.00
  OriGene Technologies, Inc.

Glutamate dehydrogenase 1 antibody

  GLUD1 Rabbit anti-Bovine Polyclonal Antibody  
Glutamate dehydrogenase 1 Rabbit IgG Purified Bov, Hu E, P, WB
0.5 mg / €400.00
  OriGene Technologies, Inc.

Glutamate dehydrogenase 1 antibody

Glutamate dehydrogenase 1 Rabbit IgG Azide Free Bov Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €240.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 antibody

Glutamate dehydrogenase 1 Rabbit IgG Biotin Bov Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €350.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 antibody

Glutamate dehydrogenase 1 Rabbit IgG Aff - Purified Bov Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €440.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 antibody

Figure 1. Western blot analysis is shown using anti-Bovine Glutamate Dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 µm nitrocellulose. The blot was incubated with a 1:1,000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye(TM) 800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at room temperature. IRDye(TM) 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit Serum Bov E, WB
2 ml / €1,180.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

GLUD1 - human gene knockout kit via CRISPR

GLUD1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GLUD1 - human gene knockout kit via CRISPR

GLUD1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GLUD1 - human gene knockout kit via CRISPR

GLUD1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GLUD1 - human gene knockout kit via CRISPR

GLUD1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Glud1 - mouse gene knockout kit via CRISPR

Glud1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Glud1 - mouse gene knockout kit via CRISPR

Glud1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Glud1 - mouse gene knockout kit via CRISPR

Glud1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Glud1 - mouse gene knockout kit via CRISPR

Glud1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Glutamate dehydrogenase 1

  Recombinant protein of human glutamate dehydrogenase 1 (GLUD1)  
Glutamate dehydrogenase 1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Glutamate dehydrogenase 1 (54-558, His-tag)

Glutamate dehydrogenase 1 Human Purified > 80 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Glutamate dehydrogenase 1 (54-558, His-tag)

Glutamate dehydrogenase 1 Human Purified > 80 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Items 1 to 20 of 26 total

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