Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C)
Background of Glutaminase kidney isoform antibody
Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC 22.214.171.124) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.[supplied by OMIM]
Immunofluorescence of monoclonal antibody to GLS on HeLa cell. [antibody concentration 10 ug/ml]
AP18036PU-N GLS Antibody staining of Formalin-fixed, Paraffin-Embedded Human brain tissue using peroxidase-conjugated secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for Immunohistochemistry. clinical relevance has not been evaluated.
Human ACHN; WB Suggested Anti-GLS Antibody Titration: 0.2-1 ug/ml. Positive Control: ACHN cell lysate. GLS is strongly supported by BioGPS gene expression data to be expressed in Human ACHN cells.; GLS antibody - C-terminal region (ARP55098_P050) in Human ACHN cells using Western Blot
GLS Antibody (C-term) (GTX81012) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
GLS Antibody (C-term) (GTX81012) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of GLS Antibody (C-term) (GTX81012) with Hela cell followed by Alexa Fluor搴?489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin embedded rat liver labeled with Rabbit Anti Glutaminase Polyclonal Antibody, Unconjugated (bs-4688R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.